Coimmobilization of heparin/fibronectin mixture on titanium surfaces and their blood compatibility

Li, Guicai; Zhang, Fengming; Liao, Yuzhen; Yang, Ping; Huang, Nan

doi:10.1016/j.colsurfb.2010.07.016

Cited by 34 publications

(22 citation statements)

References 43 publications

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“…However, although heparin is assembled to Ti surface, the heparin could restrain the adhesion and activation of platelets. The results are consistent with observations reported by others 13, 33. After incubation for 2 h, there were more aggregates and pseudopodia of platelets on Ti and Ti–OH surface, but the platelets on LBL surface remained mainly constant.…”

Section: Resultssupporting

confidence: 93%

Influence of a layer‐by‐layer‐assembled multilayer of anti‐CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface

Liu

Chen

et al. 2012

J Biomedical Materials Res

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The endothelialization of the metal surface of vascular stents came into focus as a new method for improving the biocompatibility of intravascular stents. This article has its focus on building a biofunctional layer on the activated titanium surface with anti-CD34 antibody, vascular endothelial growth factor (VEGF), and heparin by a layer-by-layer (LBL) self-assembly technique, to promote the endothelialization of the surface. When compared with titanium surface, the number of adhered endothelial progenitor cells (EPCs) on LBLs increased 47.1% after 5 days culture, meanwhile the proliferation rate of EPCs on LBLs during 5 days culture also exhibit significantly improvement. The results of blood compatibility evaluation clearly show that the LBLs reduce the number of adhered and activated blood platelets (adhered: Ti 83% vs. LBL <20% and activated: Ti 57% vs. LBL 19%), and the activated partial thromboplastin time of the LBL surface prolonged about 20 s in compared with platelet-poor plasma. The assembled LBL thus improves the thromboresistance of the titanium surface. The presented biofunctional multilayer of anti-CD34, VEGF, and heparin on titanium can significantly improve the blood compatibility and the endothelialization of a medical device. The biofunctional layer supports generation of a new endothelium onto titanium surface by capturing EPCs and oriented differentiation.

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Section: Resultssupporting

confidence: 93%

Influence of a layer‐by‐layer‐assembled multilayer of anti‐CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface

Liu

Chen

et al. 2012

J Biomedical Materials Res

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“…Heparin and heparin‐mimicking polymers have been widely used to improve the anticoagulant ability of materials. Li et al immobilized a mixture of heparin and fibronectin onto titanium substrate via physisorption or covalent coimmobilization, the APTT value was prolonged from 45 to about 120 s . Wang et al covalently grafted heparin onto silk fibroin and then blended with chitosan to prepare dual‐functional composite membrane with anticoagulant and antibacterial properties, and the clotting times for the composite membrane surpassed the instrument limit set (APTT, 120 s; TT, 120 s) .…”

Section: Resultsmentioning

confidence: 99%

Super-Anticoagulant Heparin-Mimicking Hydrogel Thin Film Attached Substrate Surfaces to Improve Hemocompatibility

Cui

Jiang

et al. 2016

Macromol. Biosci.

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In this study, heparin-mimicking hydrogel thin films are covalently attached onto poly(ether sulfone) membrane surfaces to improve anticoagulant property. The hydrogel films display honeycomb-like porous structure with well controlled thickness and show long-term stability. After immobilizing the hydrogel films, the membranes show excellent anticoagulant property confirmed by the activated partial thromboplastin time values exceeding 600 s. Meanwhile, the thrombin time values increase from 20 to 61 s as the sodium allysulfonate proportions increase from 0 to 80 mol%. In vitro investigations of protein adsorption and blood-related complement activation also confirm that the membranes exhibit super-anticoagulant property. Furthermore, gentamycin sulfate is loaded into the hydrogel films, and the released drug shows significant inhibition toward E. coli bacteria. It is believed that the surface attached heparin-mimicking hydrogel thin films may show high potential for the applications in various biological fields, such as blood contacting materials and drug loading materials.

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“…In addition, more efforts on heparin research have been made to develop the cross-linking multilayer, such as heparin/fibronectin (196,197), alginate/heparin (188), collagen/heparin (198), and dexamethasone-heparin systems (199), which benefit from the cooperative effects of heparin and the other molecules. Although heparin has been used clinically for treatment of thrombosis, it suffered from its short duration within compartments in vivo that require long-term anticoagulation.…”

Section: Heparin-bound Coatingsmentioning

confidence: 99%

Biocompatible Coating

Qiao

Liu

2014

Comprehensive Materials Processing

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Coimmobilization of heparin/fibronectin mixture on titanium surfaces and their blood compatibility

Cited by 34 publications

References 43 publications

Influence of a layer‐by‐layer‐assembled multilayer of anti‐CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface

Influence of a layer‐by‐layer‐assembled multilayer of anti‐CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface

Super-Anticoagulant Heparin-Mimicking Hydrogel Thin Film Attached Substrate Surfaces to Improve Hemocompatibility

Biocompatible Coating

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