Coinfection with Fusobacterium nucleatum can enhance the attachment and invasion of Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans to human gingival epithelial cells

Li, Yan; Guo, Hongmei; Wang, Xijun; Lu, Yang; Yang, Changzheng; Yang, Pishan

doi:10.1016/j.archoralbio.2015.06.017

Cited by 50 publications

(53 citation statements)

References 22 publications

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“…It was already shown that different oral pathogens, e.g. P. gingivalis, F. nucleatum and A. actinomycetemcommitans , are able to enter different host cell types183839. In our current study, we proved the direct interaction of P. intermedia and T. forsythia with hDFSCs in vitro .…”

Section: Discussionsupporting

confidence: 64%

Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

Hieke¹,

Kriebel²,

Engelmann³

et al. 2016

Sci Rep

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Periodontitis is characterized by inflammation associated with the colonization of different oral pathogens. We here aimed to investigate how bacteria and host cells shape their environment in order to limit inflammation and tissue damage in the presence of the pathogen. Human dental follicle stem cells (hDFSCs) were co-cultured with gram-negative P. intermedia and T. forsythia and were quantified for adherence and internalization as well as migration and interleukin secretion. To delineate hDFSC-specific effects, gingival epithelial cells (Ca9-22) were used as controls. Direct effects of hDFSCs on neutrophils (PMN) after interaction with bacteria were analyzed via chemotactic attraction, phagocytic activity and NET formation. We show that P. intermedia and T. forsythia adhere to and internalize into hDFSCs. This infection decreased the migratory capacity of the hDFSCs by 50%, did not disturb hDFSC differentiation potential and provoked an increase in IL-6 and IL-8 secretion while leaving IL-10 levels unaltered. These environmental modulations correlated with reduced PMN chemotaxis, phagocytic activity and NET formation. Our results suggest that P. intermedia and T. forsythia infected hDFSCs maintain their stem cell functionality, reduce PMN-induced tissue and bone degradation via suppression of PMN-activity, and at the same time allow for the survival of the oral pathogens.

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Section: Discussionsupporting

confidence: 64%

Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

Hieke¹,

Kriebel²,

Engelmann³

et al. 2016

Sci Rep

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“…nucleatum invades into gingival epithelial cells via a zipper mechanism [32], and a pre-FadA-mature FadA complex mediates attachment and invasion of F. nucleatum into gingival epithelial cells [20]. Infection of gingival epithelial cells by F. nucleatum increases the expression of antimicrobial peptides and cytokines, such as human beta defensin (HBD)-2, HBD-3, interleukin (IL)-6, and IL-8 [22,24,32,33]. In particular, the induction of HBD-2, HBD-3, and IL-8 production by F. nucleatum depends on bacterial invasion and endosomal maturation [24,34].…”

Section: Discussionmentioning

confidence: 99%

“…F . nucleatum has been shown to enhance the invasion of P. gingivalis [21,22]. However, the effect of P. gingivalis on F. nucleatum invasion has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells

Jung

Jun

Choi

2017

Journal of Oral Microbiology

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Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as P. gingivalis and early colonizers during the development of dental biofilms. The aim of this study was to investigate how P. gingivalis, in particular its gingipains, influences the invasion of coinfecting F. nucleatum into gingival epithelial cells. When invasion of F. nucleatum was analyzed after 4 h of infection, invasion of F. nucleatum was suppressed in the presence of P. gingivalis compared with during monoinfection. However, coinfection with a gingipain-null mutant of P. gingivalis did not affect invasion of F. nucleatum. Inhibition of PI3K reduced invasion of F. nucleatum. P. gingivalis inactivated the PI3K/AKT pathway, which was also dependent on gingipains. Survival of intracellular F. nucleatum was promoted by P. gingivalis with Arg gingipain mutation. The results suggest that P. gingivalis, in particular its gingipains, can affect the invasion of coinfecting F. nucleatum through modulating intracellular signaling of the host cells.

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“…Coinfection with Fusobacterium nucleatum can enhance the pathogenicity of PG by allowing the invasion of gingival epithelial cells 11. In our case this mechanism was likely responsible of transitory bacteraemia and subsequent pericardial infection favoured by chronic immunosuppression.…”

Section: Discussionmentioning

confidence: 55%

Molecular diagnosis of opportunistic pericardial infection in a patient treated with adalimumab: the role of next-generation sequencing

et al. 2016

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Biological immune-modulator drugs, especially inhibitors of tumor necrosis factor-α, are frequently encountered in modern clinical practice and opportunistic infections are therefore a common concern. Infective pericarditis has been described as a complication of these treatments with possible life-threatening consequences. In similar cases cultures may isolate multiple opportunistic bacteria from the pericardial fluid without specific identification of the responsible germ, representing a problem for targeted antibiotic therapy. We present a case of acute pericarditis evolving in pericardial constriction and cardiac tamponade in a patient treated with adalimumab for psoriatic arthritis overlapping with recurrent polychondritis. Next-generation sequencing allowed the identification of a common oral pathogen as the aetiological agent confirming its role in the identification of species that can be overlooked by common microbiological techniques.

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Coinfection with Fusobacterium nucleatum can enhance the attachment and invasion of Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans to human gingival epithelial cells

Cited by 50 publications

References 22 publications

Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells

Molecular diagnosis of opportunistic pericardial infection in a patient treated with adalimumab: the role of next-generation sequencing

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