Colchicine. New uses of an old, old drug

Malkinson, Frederick D.

doi:10.1001/archderm.118.7.453

Cited by 67 publications

(31 citation statements)

References 49 publications

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“…Although inhibitory effects of microtubule depolymerizers on mast cell degranulation have been observed, most studies have focused primarily on the effects of such depolymerizers on the process of granular transport (13)(14)(15). In the present study, we confirmed that two microtubule depolymerizers, nocodazole and colchicine, reduced degranulation in RBL-2H3 cells and BMMCs (Fig.…”

Section: Discussionsupporting

confidence: 83%

“…Microtubules are generally recognized to play a number of roles in mitosis, in the maintenance of organelle structures, in cell mobility, and in granular transport. As regards mast cell degranulation, the role played by microtubules in granular transport has been studied (13)(14)(15). Apart from their role in granular transport, it is well known that the microtubules are necessary for maintaining membrane-enclosed organelles at particular positions.…”

Section: Microtubule Disruption Suppresses Allergic Response Through mentioning

confidence: 99%

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Microtubule Disruption Suppresses Allergic Response through the Inhibition of Calcium Influx in the Mast Cell Degranulation Pathway

Oka

Hori

Ozaki

2005

The Journal of Immunology

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Mast cells are secretory cells that release their granules, which contain inflammatory mediators. Some recent data suggested that cytoskeletons play a role in this process. However, the role of microtubules in Ca2+ signaling has not yet been well defined. In this study, we demonstrate that the microtubule cytoskeleton is important to maintain Ca2+ influx in the degranulation pathway of mast cells, using the microtubule depolymerizers nocodazole and colchicine. The microtubule depolymerizers inhibited Ag-induced degranulation in RBL-2H3 cells and bone marrow-derived mast cells. When the cells were stimulated with Ag in the presence of the microtubule depolymerizers, the Ca2+ influx was decreased without affecting Ca2+ release from the endoplasmic reticulum (ER). Capacitative Ca2+ entry, which was induced by inhibitors of Ca2+-ATPase in the ER membrane, thapsigargin and cyclopiazonic acid, was also decreased by nocodazole. Fluorescent probe analysis demonstrated that nocodazole disrupted microtubule formation and changed the cytoplasmic distribution of the ER. The microtubule depolymerizers attenuated the passive cutaneous anaphylaxis reaction in back skin of Sprague Dawley rats. These results suggest that the microtubule cytoskeleton in mast cells is important to maintain Ag-induced capacitative Ca2+ entry, which is responsible for degranulation and the allergic response.

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Section: Discussionsupporting

confidence: 83%

Section: Microtubule Disruption Suppresses Allergic Response Through mentioning

confidence: 99%

Microtubule Disruption Suppresses Allergic Response through the Inhibition of Calcium Influx in the Mast Cell Degranulation Pathway

Oka

Hori

Ozaki

2005

The Journal of Immunology

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“…The alkaloid is present in corns and seeds of crocuslike plants. Colchicine is best known for its preventive action against gout, but it also reduces pain and swelling in degenerative and immunological infl ammatory disease (Malkinson, 1982).…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory activity of alkaloids: a twenty-century review

Barbosa-Filho¹,

Piuvezam²,

Moura³

et al. 2006

Rev. bras. farmacogn.

170

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RESUMO: "Atividade anti-infl amatória de alkalóides: Uma revisão do século XX". Muitas substâncias que interferem na resposta infl amatória têm sido isoladas de plantas. Esta revisão mostra alguns alcalóides de origem vegetal que no período de 1907-2000 foram avaliados quanto a uma possível atividade anti-infl amatória. Os alcalóides foram classifi cados em subgrupos de acordo com suas estruturas químicas e os dados farmacológicos foram obtidos de diferentes modelos experimentais. Dos 171 alcalóides avaliados, 137 apresentaram atividade anti-infl amatória, e, entre eles, os alcalóides do tipo isoquinolínicos foram os mais estudados. O modelo de edema de pata induzido por carragenina foi o mais empregado para avaliação da atividade anti-infl amatória. Nesta revisão 174 referências foram consultadas. Unitermos: Alcalóides, atividade anti-infl amatória, infl amação, modelos experimentais.ABSTRACT: Many substances which interfere with the infl ammatory response have been isolated from plants. This review shows some alkaloids of vegetal origin which in the period of 1907 to 2000 were evaluated regarding a possible anti-infl ammatory activity. The alkaloids were classifi ed in sub-groups in accordance with their chemical structures and the pharmacological data were obtained from different experimental models. Of the 171 evaluated alkaloids, 137 presented anti-infl ammatory activity, and among those, the isoquinoline type was the most studied. The Carrageenin-induced paw edema was the most used model for evaluating the anti-infl ammatory activity. In this review, 174 references were cited.

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“…Colchicine inhibits the PMN chemotaxis [36], and suppresses inflammatory reac tions [37], It has in some studies been found of benefit in patients with PV [38], general pustular psoriasis [39], and PPP [40], although the effect on PPP in one controlled study was dubious [41]. Colchicine seemed to benefit 2 of the present patients with PPP and 6 patients without PPP, and their PMN chemotaxis became normal during the ther apy, consistent with a possible pathogenetic significance of the PMNs.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil Chemotaxis in Patients with Pustulotic Arthro-Osteitis

Jurik¹,

Ternowitz²,

Graudal³

1988

Dermatology

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The chemotactic response of peripheral blood polymorphonuclear leukocytes (PMNs) to N-formyl-methionyl-leucyl-phenylalanine was determined in four groups of persons (I) 19 patients with pustulosis palmoplantaris (PPP) and skeletal disorder (pustulotic arthro-osteitis); (II) 15 patients with a similar anterior chest wall involvement, but no PPP; (III) 9 patients with PPP, but without skeletal involvement, and (IV) 69 healthy adults (controls). The chemotactic activity of PMNs was found to be significantly increased in groups I–III, and patients with a similar osteoarthropathy, but no PPP compared with the controls. Furthermore the patients with pustulotic arthrosteitis had enhanced chemotactic activity compared with the patients with PPP only. This indicates that both skeletal involvement and PPP are somehow related to the function of PMNs. Colchicine was found of benefit in 2 of 3 group I, and in 6 group II patients, and it normalized their PMN chemotaxis.

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Colchicine. New uses of an old, old drug

Cited by 67 publications

References 49 publications

Microtubule Disruption Suppresses Allergic Response through the Inhibition of Calcium Influx in the Mast Cell Degranulation Pathway

Microtubule Disruption Suppresses Allergic Response through the Inhibition of Calcium Influx in the Mast Cell Degranulation Pathway

Anti-inflammatory activity of alkaloids: a twenty-century review

Neutrophil Chemotaxis in Patients with Pustulotic Arthro-Osteitis

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