Cold-Induced Injury to Porcine Corneal Endothelial Cells and Its Mediation by Chelatable Iron

Rauen, Ursula; Kerkweg, Uta; Wusteman, Monica; Groot, Herbert de

doi:10.1097/01.ico.0000167880.96439.c6

Cited by 33 publications

(37 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several possible explanations of such a phenomenon: e.g., an increased turnover or synthesis of the cellular proteins, a malfunction of some of the enzymatic systems in cells or a transient loss of the integrity of cellular membranes leading to the outflow of amino acids to the medium. The latter option should be particularly considered regarding the previous reports on the cornea cells death that increased during the cold storage [19,[21][22][23]. In addition, the results of this study also support this mechanism of the deprivation of biochemical compounds in corneas.…”

Section: Discussionsupporting

confidence: 68%

Cellular Stress after Transferring Human Cornea from in vivo to in vitro Milieu - a Metabolomic Approach

Kryczka¹

2014

CTB

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 68%

Cellular Stress after Transferring Human Cornea from in vivo to in vitro Milieu - a Metabolomic Approach

Kryczka¹

2014

CTB

View full text Add to dashboard Cite

“…Storage times up to 14 days are claimed but the epithelium is less well preserved than the endothelium, and many eye banks prefer not to exceed 7 days of storage. Metabolic support [30], countering damage from reactive oxygen species [31], nitric oxide synthase inhibitors [32], and use of non-ionic surfactants (Poloxamer 188) [33] have all been reported to be beneficial during hypothermic storage of corneas; but overall storage times have yet to be improved by these approaches.…”

Section: Donor Selection and Tissue Qualitymentioning

confidence: 99%

Preservation of Human Cornea

Armitage¹

2011

Transfus Med Hemother

123

102

View full text Add to dashboard Cite

The successful outcome of the majority of corneal transplants depends on the presence of a viable corneal endothelium. This monolayer of cells lines the inner surface of the cornea and its primary function is to maintain corneal transparency by controlling the hydration of the collagenous stromal layer. Since human corneal endothelial cells do not readily proliferate, preservation of the endothelium is a primary aim of methods of corneal storage. Although some cryopreserved corneas have been transplanted successfully, the complexity of the cryopreservation technique and its potential for causing endothelial damage have limited its application. Hypothermia (2–8°C) is the most commonly applied method of storage, which allows storage for 7–14 days. Organ culture (28–37°C), which extends storage time to 4 weeks, is used widely in European eye banks. Graft outcomes for corneas stored by these two techniques appear similar.

show abstract

“…Although storage of those vessels induces injury, mechanisms of injury due to cold storage have only recently been extensively studied. Injury due to cold storage and prevention from injury have already been exhibited for corneal cells, cultured endothelial cells, hepatocytes, rat mesenteric artery, segments of rat and pig aorta and lung epithelial cells [1][2][3]. The same is true for the human internal thoracic artery [4].…”

Section: Introductionmentioning

confidence: 55%

“…One concern was histidine, which serves as buffer in Custodiol but enhances iron formation at high concentrations [2]. For this reason, in TiProtec histidine was replaced by N-acetylhistidine (pK = 7.2), which has a different affinity for redox-active iron and forms more stable complexes [2].…”

Section: Study Limitationsmentioning

confidence: 99%

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Schipke¹

2016

AVS

View full text Add to dashboard Cite

ObjectiveGreat Saphenous Veins (SVs) are widely used in coronary and in non-coronary surgery, and the need for vascular transplants is increasing. In older pre¬servation solutions, hypothermia leads to damage induced by reactive oxygen species. Iron chelators in the newer Tissue Protection (TiProtec) solution shall par¬ticularly protect vessels by preventing from that damage.It was the aim to compare three preservation solutions to maintain function and viability of human SVs. MethodsVein segments were randomly assigned to Krebs-Henseleit Buffer (KHB), Viaspan (UW), or TiProtec. Contraction/relaxation in organ baths was assessed at 3, 24, 48 and 72h after removal. The protocol included three steps: receptor-independent contraction (KCl: 80μM), receptor-dependent contraction (PE: ≤30μM), endothelium-dependent relaxation (ACh: ≤20μM). Morphology: Vein segments were incubated with propidium iodide and 5-chloromethylfluorescein diacetate. Viability was assessed with fluorescence using laser scanning microscopy. ResultsKHB was inappropriate after only 3h. In TiProtec, SVs contraction/relaxation had increased further at 72h, but not in both other groups. More necrosis was found in KHB SVs than in UW or TiProtec at 3h. More viable cells were found in UW and in TiProtec SVs on day-1, while only few cells were viable in KHB on day-10. Summary and conclusionEven short storage of SVs in KHB is deleterious. UW preserved func¬tion better over time. The best functional and morphological results yielded TiProtec over time, and the amount of viable cells correlated with function. TiProtec seems a promising alternative for short-and mid-term hypothermic vein preservation.

show abstract

Cold-Induced Injury to Porcine Corneal Endothelial Cells and Its Mediation by Chelatable Iron

Cited by 33 publications

References 43 publications

Cellular Stress after Transferring Human Cornea from in vivo to in vitro Milieu - a Metabolomic Approach

Cellular Stress after Transferring Human Cornea from in vivo to in vitro Milieu - a Metabolomic Approach

Preservation of Human Cornea

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Contact Info

Product

Resources

About