Cold Precipitable Haemagglutinating Factor in Serum from patients with Rheumatoid Arthritis

Svartz, Nanna; Schlossmann, K

doi:10.1136/ard.14.2.191

Cited by 46 publications

(11 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these reactions have been employed as diagnostic procedures and the factor or group of factors involved has been termed the "rheumatoid factor." A number of observations (1,2) have demonstrated that the rheumatoid factor is a y-globulin with the solubility properties of a euglobulin. More recently, ultracentrifuge experiments (3,4) have indicated that the rheumatoid factor is a high molecular weight -y-globulin that exists in serum and plasma in the form of a complex.…”

mentioning

confidence: 99%

Studies on the Isolation and Characterization of the “Rheumatoid Factor”

Kunkel¹,

Franklin²,

Müller‐Eberhard³

1959

J. Clin. Invest.

111

View full text Add to dashboard Cite

Considerable evidence has been accumulated which indicates that a large proportion of the sera from patients with rheumatoid arthritis contains a factor which has the property of agglutinating particulate bodies such as red blood cells or latex particles when they are coated with -y-globulin or antibodies. Some of these reactions have been employed as diagnostic procedures and the factor or group of factors involved has been termed the "rheumatoid factor." A number of observations (1, 2) have demonstrated that the rheumatoid factor is a y-globulin with the solubility properties of a euglobulin. More recently, ultracentrifuge experiments (3, 4) have indicated that the rheumatoid factor is a high molecular weight -y-globulin that exists in serum and plasma in the form of a complex. This complex can be visualized directly by means of the analytical ultracentrifuge in whole serum and in -y-globulin and euglobulin fractions and has a corrected sedimentation rate (s rate) of approximately 22S. Previous studies (4, 5) on the dissociation of the 22S complex by means of acid and urea have demonstrated that it is composed of two major constituents, one a 7S component and'the other 19S. The latter was found to be highly active by the serological reactions for rheumatoid factor. The rheumatoid factor thus appeared to belong to the 19S class of y-globulins which is known to include certain antibodies as well as the Waldenstrom type macroglobulins (6, 7).Considerable purification of the rheumatoid factor has been achieved by cellulose ion exchange chromatography (8,9), by elution from pneumococcus-antipneumococcus complexes (10), by elution from sensitized sheep cells (11), by ultrafiltration with membranes of graded porosity (12), and by preparative ultracentrifugation (4, 13), but isolation in sufficient amounts to determine the complete physical and chemical properties of the rheumatoid factor has not been reported. The observations that rheumatoid factor precipitated directly with y-globulin (14) which had been aggregated by the procedures of isolation or heating at 630C. for 10 minutes (3, 13) offered a method of ready concentration from large volumes of serum. Furthermore the direct visualization of rheumatoid factor in the ultracentrifuge offered a means of observing purification particularly from certain unique sera where it represented a substantial portion of the total y-globulin.In the present study rheumatoid factor has been obtained in a high state

show abstract

mentioning

confidence: 99%

Studies on the Isolation and Characterization of the “Rheumatoid Factor”

Kunkel¹,

Franklin²,

Müller‐Eberhard³

1959

J. Clin. Invest.

111

View full text Add to dashboard Cite

show abstract

“…Monoclonal gammopathics associated with cryo globulinemia include multiple myeloma [6,9,14] and macroglobulinemia [3,8], Cryoglobulinemia may also occur in such polyclonal gammopathies as rheumatoid arthritis and malaria [13,15]. Finally, cryoglobulinemia may occur without any obvious underlying disease, the so-called essential or idiopathic cryoglobulinemia [7,12].…”

Section: Discussionmentioning

confidence: 99%

Studies on Cryogelglobulinemia Associated with IgA Myeloma Protein

Slavin¹,

Suriano²,

Dreesman³

1971

Int Arch Allergy Immunol

View full text Add to dashboard Cite

A patient with an extramedullary plasmacytoma was diagnosed as having multiple myeloma associated with type I (κ) IgA cryogelglobulin. Treatment of the patient with cyclophosphamide resulted in a marked decrease in the serum IgA level with a concomitant increase in serum gelling time. Physical studies of the whole serum and the partially purified cryogelglobulin showed it had a sedimentation coefficient of 8.8S and an approximate isoelectric pH of 5.5. Alteration of disulfide bonds without alkylation greatly increased the gelling time of the cryogelglobulin.

show abstract

“…Ziff and his colleagues found inhibitor in pooled human gamma globulin which was active in vivo in inhibiting the agglutination reaction, and in causing inhibition of the reaction to reappear in rheumatoid sera (Ziff et al, 1956). Cold precipitation of serum as a means of fractionation yields a globulin of high activity (Svartz and Schlossmann, 1955) and appears to be almost as effective as dialysis in removing inhibitor and factors giving false positive reactions.…”

Section: Sheep Cell Agglutinationmentioning

confidence: 99%

“…Rose et al (1948) (1953) found a water-precipitated gamma globulin to contain the active factor. The cold-precipitated active fraction of Svartz and Schlossmann (1955) is also a gamma globulin, but as the result of zone electrophoresis studies on starch columns Svartz (1956) believes some activity is due also to a fraction in the beta region. By ethanol precipitation of serum, Heller et al (1955) found activity specifically in fraction III, predominantly beta globulin, although there is no correlation between betaglobulin concentration and agglutinating activity.…”

Section: Nature Of the Reactionmentioning

confidence: 99%

Serological Agglutination and Precipitin Mechanisms in Rheumatoid Arthritis

Dresner

1957

Postgraduate Medical Journal

View full text Add to dashboard Cite

Cold Precipitable Haemagglutinating Factor in Serum from patients with Rheumatoid Arthritis

Cited by 46 publications

References 5 publications

Studies on the Isolation and Characterization of the “Rheumatoid Factor”

Studies on the Isolation and Characterization of the “Rheumatoid Factor”

Studies on Cryogelglobulinemia Associated with IgA Myeloma Protein

Serological Agglutination and Precipitin Mechanisms in Rheumatoid Arthritis

Contact Info

Product

Resources

About