Cold tolerance abilities of two entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora

Ali, Farman; Wharton, David A.

doi:10.1016/j.cryobiol.2012.10.004

Cited by 25 publications

(37 citation statements)

References 27 publications

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“…After thawing the infective juveniles were motile, pathogenic to their insect host as before and produced the next generation [6]. This suggests that S .…”

Section: Discussionmentioning

confidence: 80%

“…At −2°C, and lower temperatures, all the nematodes turned dark and stopped moving, suggesting that their survival in the cooling block experiments at these temperatures [6] was due to freezing tolerance. Cold stage observations on S. feltiae have been reported by Brown and Gaugler [5].…”

Section: Discussionmentioning

confidence: 99%

“…In our previous experiments, we showed that S. feltiae IJs could survive sub-zero temperatures [6] but not whether the nematodes themselves or just the water surrounding them froze. Here we determine the mechanism of cold tolerance of S. feltiae IJs based on morphological changes at the cellular level after freezing them under various conditions.…”

Section: Introductionmentioning

confidence: 89%

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Intracellular Freezing in the Infective Juveniles of Steinernema feltiae: An Entomopathogenic Nematode

Ali

Wharton

2014

PLoS ONE

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Taking advantage of their optical transparency, we clearly observed the third stage infective juveniles (IJs) of Steinernema feltiae freezing under a cryo-stage microscope. The IJs froze when the water surrounding them froze at −2°C and below. However, they avoid inoculative freezing at −1°C, suggesting cryoprotective dehydration. Freezing was evident as a sudden darkening and cessation of IJs' movement. Freeze substitution and transmission electron microscopy confirmed that the IJs of S. feltiae freeze intracellularly. Ice crystals were found in every compartment of the body. IJs frozen at high sub-zero temperatures (−1 and −3°C) survived and had small ice crystals. Those frozen at −10°C had large ice crystals and did not survive. However, the pattern of ice formation was not well-controlled and individual nematodes frozen at −3°C had both small and large ice crystals. IJs frozen by plunging directly into liquid nitrogen had small ice crystals, but did not survive. This study thus presents the evidence that S. feltiae is only the second freeze tolerant animal, after the Antarctic nematode Panagrolaimus davidi, shown to withstand extensive intracellular freezing.

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“…After thawing the infective juveniles were motile, pathogenic to their insect host as before and produced the next generation [6]. This suggests that S .…”

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

See 1 more Smart Citation

Intracellular Freezing in the Infective Juveniles of Steinernema feltiae: An Entomopathogenic Nematode

Ali

Wharton

2014

PLoS ONE

Self Cite

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“…Therefore, we should pay more attention to the detrimental effects of cold storage of cadavers at temperature‐sensitive points, and not to transfer the cadavers to cool storage rooms during this period. In addition, it was found that exposure to slow dehydration not only improves extreme desiccation survival, but also promotes enhanced cold tolerance . This could be one of the strategies for the survival of S. carpocapsae populations when developing under extreme environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Desiccation and cold storage of Galleria mellonella cadavers and effects on in vivo production of Steinernema carpocapsae

Wang

Feng

et al. 2013

Pest. Manag. Sci.

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“…Biological pest control agents may become increasingly important for ensuring crop protection in the future [32]. A number of factors currently limit the commercial applicability of H. bacteriophora, including their short shelf life, susceptibility to environmental stress and limited insect tropism [12,33]. Accurate genome annotation will assist in the Page 22…”

Section: Ag 3') Introns While Most Genomes Have a Low Proportion Of mentioning

confidence: 99%

Improving the annotation of the Heterorhabditis bacteriophora genome

McLean

Berger

Laetsch

et al. 2017

Preprint

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Genome assembly and annotation remains an exacting task. As the tools available for these tasks improve, it is useful to return to data produced with earlier instances to assess their credibility and correctness. The entomopathogenic nematode Heterorhabditis bacteriophora is widely used to control insect pests in horticulture. The genome sequence for this species was reported to encode an unusually high proportion of unique proteins and a paucity of secreted proteins compared to other related nematodes. We revisited the H. bacteriophora genome assembly and gene predictions to ask whether these unusual characteristics were biological or methodological in origin. We mapped an independent resequencing dataset to the genome and used the blobtools pipeline to identify potential contaminants. While present (0.2% of the genome span, 0.4% of predicted proteins), assembly contamination was not significant. Re-prediction of the gene set using BRAKER1 and published transcriptome data generated a predicted proteome that was very different from the published one. The new gene set had a much reduced complement of unique proteins, better completeness values that were in line with other related species' genomes, and an increased number of proteins predicted to be secreted. It is thus likely that methodological issues drove the apparent uniqueness of the initial H. bacteriophora genome annotation and that similar contamination and misannotation issues affect other published genome assemblies.not peer-reviewed)

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Cold tolerance abilities of two entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora

Cited by 25 publications

References 27 publications

Intracellular Freezing in the Infective Juveniles of Steinernema feltiae: An Entomopathogenic Nematode

Intracellular Freezing in the Infective Juveniles of Steinernema feltiae: An Entomopathogenic Nematode

Desiccation and cold storage of Galleria mellonella cadavers and effects on in vivo production of Steinernema carpocapsae

Improving the annotation of the Heterorhabditis bacteriophora genome

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