Colipase enhances hydrolysis of dietary triglycerides in the absence of bile salts.

The complete digestion of human milk triacylglycerol in vitro requires gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase.

“…In fact, pancreatic colipase-dependent lipase is on its own virtually without activity against native human milk globule triacylglycerol (13, 14, 16). Similar observations have been made with synthetic triacylglycerol emulsion particles covered with phospholipid or dietary proteins such as f3-lactoglobulin, lactoferrin, and albumin (29,(32)(33)(34). It seems as if the presence of other surface active components denies the lipase access to the triacylglycerol.…”

“…It seems as if the presence of other surface active components denies the lipase access to the triacylglycerol. There are however mechanisms by which the inhibition of colipasedependent lipase can be reduced, i.e., addition of colipase, of phospholipase A2, and of bile salts (14,15,33,34). However, studies using either an artificial phospholipid-covered emulsion (Intralipid) or human milk as substrate have shown that when added in physiologic concentrations neither of these factors alone is enough to effectively relieve the inhibition (16,29).…”

Influence of Colipase on the Turbidimetric Determination of Pancreatic Lipase Catalytic Activity

“…On the basis of recent experiments on the Inhibition of porcine lipase by some proteins (12,18) and our finding that serum competitively inhibits human lipase, it appears reasonable to assume that there is a general physical effect of proteins on lipase action rather than a specific Inhibition of the enzyme: inhibiiion by serum is due to competition of proteins with lipase for the substrate-water interface. That is why turbidimetric assays with low Substrate concentrations are highly susceptible to Variation of the protein content of the sample.…”

Hydrolysis of Mixed Monomolecular Filme of phosphatidulchoiline/Triacylglycerol by Pancereatic Phospholipase A2

“…The simultaneous formation of ceramides and diacylglycerol in the outer layer of the cell membrane apparently triggers a hemolytic process (Colley et al [42]). Another example comes from work on pancreatic lipase and phospholipase (Blackberg et al [43]; Borgstrom et al [44]). Longchain triacylglycerol emulsified with phosphatidylcholine were found to be hydrolyzed very slowly by pancreatic lipase, even in the presence of bile salts and colipase.…”