Collaborative investigation of variables in susceptibility testing of yeasts

Pfaller, Michael A.; Rinaldi, Michael G.; Galgiani, John N.; Bartlett, Marilyn S.; Body, Barbara A.; Espinel-Ingroff, Ana; Fromtling, Robert A.; Hall, Gerri S.; Hughes, Carolyn E.; Odds, Frank C.

doi:10.1128/aac.34.9.1648

Cited by 181 publications

(154 citation statements)

References 21 publications

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“…Idealmente, las pruebas de sensibilidad antifúngica deben ser fáciles de realizar, con una buena relación coste-eficacia y que proporcionen puntos de corte de las CIM que sean reproducibles y puedan determinarse con prontitud tras un corto período de incubación 19,20 . Para obviar los inconvenientes que se presentan con el uso del método de referencia para levaduras (NCCLS, documento M27-A) por su laboriosidad, nosotros hemos determinado la sensibilidad disminuida al fluconazol mediante un método sensible, pero más rápido y económico, CHROM-FZ.…”

Section: Discussionunclassified

CHROMAgar Candida más fluconazol: comparación con técnicas de microdilución

Linares¹,

Charriel²,

Solis³

et al. 2003

Enferm Infecc Microbiol Clin

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Section: Discussionunclassified

CHROMAgar Candida más fluconazol: comparación con técnicas de microdilución

Linares¹,

Charriel²,

Solis³

et al. 2003

Enferm Infecc Microbiol Clin

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“…This variability may be due to important methodological differences among the laboratories (22). The NCCLS antifungal collaborative study using the broth macrodilution method (23), and the microdilution method (24) evaluated the effect of medium, incubation time (24 vs 48 h) and incubation temperature (30°C vs 35°C) on intra and inter-laboratory variations of MIC endpoints. The highest agreement among laboratories, including the rank order of susceptibility, was obtained with RPMI-1640 medium at 35°C and after a 24-h incubation time with antifungal compounds (25).…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activities of Propolis Collected by Different Races of Honeybees Against Yeasts Isolated From Patients With Superficial Mycoses

et al. 2005

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Abstract. Yeasts isolated from patients with superficial mycoses were tested against propolis samples collected from different regions and honeybee races. The minimum inhibitory concentration (MIC) values obtained using the agar dilution methods were compared to the diameters of growth inhibition zones by using the disk diffusion method. The results showed that Candida albicans, C. glabrata, Trichosporon spp., and Rhodotorula sp. were susceptible to low concentrations of propolis, the latter showing a higher susceptibility. Relative to the other propolis tested, the propolis sample collected by Apis mellifera caucasica possessed the highest antifungal activity against all of the superficial mycoses. In contrast, the propolis samples collected by A.m. carnica and A.m. anatolica were the least active samples. Also, the propolis sample from the Adana region is more active than samples from other regions. An increase of MIC values was accompanied by a decrease of growth inhibition zone diameters.

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“…The yeast cells were collected from the solid medium (MVM); diluted (1:10) in a counting solution containing 0.9% NaCl, 4% formaldehyde, and 4% Tween 20; vortexed to disperse the aggregated cells; and counted in a Neubauer chamber. An initial inoculum density of 10 5 cells ml Ϫ1 was obtained (16). A 0.1-ml aliquot of this suspension was then added to 0.9 ml of MVM broth, giving the desired dilutions of the drug and a final concentration of 10 4 cell ml Ϫ1 (8,14).…”

Section: Methodsmentioning

confidence: 99%

Randomly Amplified Polymorphic DNA as a Valuable Tool for Epidemiological Studies ofParacoccidioides brasiliensis

et al. 2003

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Randomly amplified polymorphic DNA (RAPD) has been successfully used to detect genetic variations among isolates of Paracoccidioides brasiliensis. However, the usefulness of this technique for assessing important parasitic properties is still unconfirmed. In the present work we further investigated the applicability of RAPD in revealing important intrinsic and extrinsic features of this fungus associated with geographical origin, time of isolation, source of clinical specimen, clinical forms of human disease and also in vitro and in vivo susceptibility to antimicrobial and antifungal drugs. The RAPD patterns allowed us to distinguish all of the analyzed strains, which included 26 clinical isolates, 2 animal isolates, and 1 environmental isolate of P. brasiliensis obtained from different geographic regions, confirming the strong discriminating power of this technique. A phenetic tree, build from the RAPD data, showed that although the two nonclinical Brazilian strains were set together the majority of the clinical Brazilian strains were randomly distributed through different sub-branches of a major cluster without any correlation to any of the parameters analyzed. A second major cluster, however, has grouped isolates from Mato Grosso and Roraima (Brazil) that not only were susceptible in vitro to trimethoprim-sulfamethoxazole but also produced a good in vivo response. These results open new vistas for epidemiological and clinical studies of P. brasiliensis.Paracoccidioides brasiliensis is a dimorphic fungus alternating between the mycelial form observed in room temperatures and the yeast form found in infected tissues or in culture at 35 to 37°C. The human paracoccidioidomycosis caused by this fungus is characterized by a range of clinical manifestations from benign or asymptomatic forms to severe and disseminated disease that is often fatal. The exact causes of the clinical pleomorphism are not understood, but it is reasonable to presume that factors associated with both the fungus and the patient are involved. In relation to the fungus, although wide biological and biochemical diversity has been observed, no clear correlation with pathogenesis has been demonstrated (3,9,12,13,17,20,23). Until now very little has been known about the genome sequences of P. brasiliensis; hence, the use of molecular approaches for isolate typing has been difficult.The development of the randomly amplified polymorphic DNA (RAPD) technique represented a landmark in the molecular characterization of diverse organisms, especially for microbial strain identifications when very few genomic sequences are available. In relation to other molecular techniques, RAPD analysis offers the advantage of requiring small amounts of DNA, simpler procedures, and the use of arbitrary primers (26). Due to these characteristics, RAPD has been widely used for human diseases to detect genomic variations among isolates of fungi such as Histoplasma capsulatum (27), Cryptococcus neoformans (19), Aspergillus fumigatus (1), and P. brasiliensis (3,9,12,13,23). ...

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Collaborative investigation of variables in susceptibility testing of yeasts

Cited by 181 publications

References 21 publications

CHROMAgar Candida más fluconazol: comparación con técnicas de microdilución

CHROMAgar Candida más fluconazol: comparación con técnicas de microdilución

Antifungal Activities of Propolis Collected by Different Races of Honeybees Against Yeasts Isolated From Patients With Superficial Mycoses

Randomly Amplified Polymorphic DNA as a Valuable Tool for Epidemiological Studies ofParacoccidioides brasiliensis

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