Collaborative study on the use of motility enrichment on modified semisolid Rappaport-Vassiliadis medium for the detection of Salmonella from foods

Zutter, Lieven De; Smedt, Jozef M. De; Abrams, Rosalyn Y.; Beckers, H. J.; Catteau, M; Borchgrave, J. de; Debevere, Johan; Hoekstra, J.; Jonkers, F.; Lenges, J.; Notermans, S.; Damme, Lut Van; Vandermeersch, R.; Verbraeken, R.; Waes, G.

doi:10.1016/0168-1605(91)90131-8

Cited by 40 publications

(15 citation statements)

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“…Under field conditions, the culture of faecal samples is considered to have a sensitivity below 50% [2]. In a collaborative study [12], it has been shown that the relative sensitivity of MSRV is 96% in naturally contaminated food samples. In mesenteric lymph node samples and in faecal samples, the combination of MSRV (selecting the more motile serotypes) with RV (selecting the non-motile serotypes) had a high relative sensitivity (98.4%), as shown in preliminary experiments [21].…”

Section: Discussionmentioning

confidence: 99%

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

et al. 2005

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-Most Salmonella control programmes are based on serological testing in the slaughterhouse. However, from a point of view of carcass contamination, it is rather the presence of Salmonella spp. in the animal at the time of slaughter that is important. The aim of this crosssectional study was to investigate the possible discrepancies between the isolation of Salmonella spp. in the mesenteric lymph nodes and the results of serological screening. In total, 1821 fattening pigs originating from 60 Belgian farrow-to-finish herds were sampled in the slaughterhouse. The serum samples were analysed using an indirect mix-ELISA for the presence of Salmonella antibodies and evaluated at 3 cut-off values namely 10, 20, and 40% Optical Density (OD). All mesenteric lymph node samples were submitted to qualitative Salmonella isolation and a representative number of isolates was serotyped. From each herd, 30 animals were screened both serologically and bacteriologically and the herd was considered as positive when at least one sample was positive. At the herd level, 83.6% (cut-off OD 40%) to 100.0% (cut-off OD 10%) of the herds from which Salmonella had been isolated were evaluated as seropositive. At the individual level, only 34.5% (cut-off OD 40%) to 82.8% (cut-off OD 10%) of the animals from which Salmonella had been isolated were seropositive. Overall, a weak agreement was found between bacteriology and serology for Salmonella diagnosis. If pig herds are categorised using serological tests in the slaughterhouse, one should be aware of the fact that slaughter pigs can still harbour Salmonella spp. in the mesenteric lymph nodes, without being detected in serological tests. The cut-off value used to evaluate a sample as serologically positive and the number of samples per herd are of major importance to classify herds correctly in order to protect human health.slaughter pig / Salmonella / bacteriology / serology * Corresponding author: nathalie.nollet@UGent.be Article published by EDP Sciences and available at

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Section: Discussionmentioning

confidence: 99%

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

et al. 2005

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“…The original formulation of Rappaport broth (1) containing malachite green as a selective agent was developed for incubation at 37 °C. A semisolid agar based upon Rappaport-Vassiliadis selective broth has meanwhile been developed commercially (6) and used for the isolation of salmonellae from food samples (7). However, Rappaport-Vassiliadis broth for selective enrichment has not been widely adopted in laboratories processing human faecal samples, probably due to its unsuitability for isolation of Salmonella typhi and the importance of using a sufficiently small inoculum so as not to interfere with selectivity.…”

Section: Improved Isolation Of Salmonellae From Faeces Using a Semisomentioning

confidence: 99%

Improved isolation of salmonellae from faeces using a semisolid rappaport-vassiliadis medium

Aspinall

Hindle

Hutchinson

1992

Eur. J. Clin. Microbiol. Infect. Dis.

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A modified semisolid Rappaport-Vassiliadis (MSRV) enrichment medium was evaluated as an alternative to Rappaport-Vassiliadis (RV) broth for culture of salmonellae from faeces. Faeces from 1544 subjects were cultured using MSRV medium, selective agars, and RV and selenite F enrichment broths. Of the 183 strains of Salmonella spp. isolated, 88% were recovered on MSRV medium, whilst only 59% were recovered using RV broth. When MSRV medium was combined with direct culture and selenite enrichment, 98.9% of salmonellae were recovered. The MSRV culture was found to be easy to read, and in most cases confirmation of organisms as Salmonella spp. could be made 24 hours after receipt of the faecal specimen.

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“…Next, the homogenates were serially diluted in 0.1% peptone water and plated on Violet Red Bile Glucose Agar ( To determine the incidence of Salmonella spp., 0.1 ml of each intestinal homogenate was inoculated in duplicate into tubes containing 10 ml of Rappaport-Vassiliadis broth (Oxoid CM669, Oxoid Ltd) and was incubated at 42°C for 2 days, as previously recommended (Anonymous, 1989;Cloak et al, 1999). Brilliant Green Agar (Oxoid CM263, Oxoid Ltd) plates were inoculated with each of the RV broths after 24 to 48 h and were incubated for 18 to 24 h at 37°C, as previously described De Zutter et al, 1991). Suspected colonies were confirmed biochemically by inoculation into Triple Sugar Iron Agar (Oxoid CM277, Oxoid Ltd) and Lysine Iron Agar (Oxoid CM381, Oxoid Ltd) slopes incubated at 37°C for 24 h. Salmonella spp.…”

Section: Measurements and Analysismentioning

confidence: 99%

Effect of different levels of crude glycerol on the morphology and some pathogenic bacteria of the small intestine in male broilers

Özdoğan

Topal

Paksuz

et al. 2014

Animal

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The gut health of poultry is closely associated with feeds and feeding. The experiment was conducted to the effect of crude glycerol addition to diets of male broilers on the bacterial microflora and morphology of their small intestines (duodenum, jejunum and ileum). A total of 120 Ross 308 broiler chicks received diets containing 0% (GLY 0), 4% (GLY 4) or 8% (GLY 8) crude glycerol for 42 days. The presence of Coliform bacteria and Enterobacteria was reduced in the duodenal tract of the broilers of GLY 4 (P < 0.001); however, the presence of Staphylococci/Micrococci in the GLY 8 was reduced relative to the other groups (P < 0.001). The presence of Salmonella spp. decreased in conjunction with the increasing quantities of glycerol (P < 0.001). Analysis of the data regarding gut morphology (epithelial cell thickness, villi length and width, and crypt length and width) indicated that the glycerol levels fed to the different groups of broilers represented statistically different results in the small intestine. In general, whereas the diet with 4% glycerol statistically affected the investigated parameters of the gut, the diet with 8% glycerol statistically affected some segments of the broiler intestines.Keywords: glycerol, broilers, bacterial microflora, villi, crypt ImplicationsAlthough glycerol can be an attractive alternative energy source for animal feed, it has its own limitations in terms of lower energy content than oils, impurities and possible effects on the metabolic activity of the animals. There are still a number of unanswered questions about glycerol. One of the questions is what is the effect of glycerol on gut health? We wanted to investigate the potential physiological effects of glycerol on the intestine of broilers. This research was aimed at determining the effects of crude glycerol levels on the bacterial microflora and morphology of the small intestines in male broilers.

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Collaborative study on the use of motility enrichment on modified semisolid Rappaport-Vassiliadis medium for the detection of Salmonella from foods

Cited by 40 publications

References 10 publications

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

Improved isolation of salmonellae from faeces using a semisolid rappaport-vassiliadis medium

Effect of different levels of crude glycerol on the morphology and some pathogenic bacteria of the small intestine in male broilers

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