Collagen-1 Membrane for Replacing the Bladder Wall

Abstract

We studied the possibility of using membrane fabricated from type 1 collagen isolated from cattle tissues (group 1) or porcine tissues (group 2) for replacement of the resected bladder wall defect in rabbits in order to retain functional volume of the organ. Satisfactory take of both types of collagen membranes with formation of competent anastomosis was observed. Histological studies revealed inflammatory process in the bladder wall at the site of contact with the implanted membrane (more pronounced in case of membranes from cattle tissues) that decreased by day 21 of the experiment. Bladder tissue ingrowth into the implant from was observed starting from day 14. The bladder capacity decreased in 7 days after surgery in both groups, presumably because of increasing tone of the organ wall resulting from surgical trauma and inflammation. In group 2, the bladder volume increased by day 14 after surgery and returned to normal by day 21, whereas in group 1 it remained below the control despite a trend to increase. These findings confirm good prospects of using collagen-1 membranes for plastic repair of the urinary bladder, the membranes from porcine collagen being more preferable.

Finding the Perfect Membrane: Current Knowledge on Barrier Membranes in Regenerative Procedures: A Descriptive Review

“…At the same time, the membrane allows the signals from the cells associated with the membrane to be transferred to the cells in the bone defect, thus creating a favorable environment for bone neo-formation [68]. They can also be easily integrated with soft tissues and are permeable, so nutrient diffusion can be conducted easily [69,70].…”

Properties of a bovine collagen type I membrane for guided bone regeneration applications

“…The membranes that induce a physiological reaction using mononuclear cells undergo an integration process and maintain their structure for 60 days. The reaction of collagen-based materials is dominated by mononuclear cells, which lead to their integration into the host tissue (12).…”

“…The advantages of collagen membranes over other natural and synthetic materials are the hemostatic functions that allow early wound stabilization; capable of promoting cell attachment and proliferation; capable of attracting fibroblasts, fibroblasts, and osteoblast, which can attach to its surface; capable of integrating with soft tissues; and permeability that facilitates the diffusion of nutrients (12). Another important advantage is that natural resorbable membranes do not require a second surgery for removal.…”

Bladder muscle regeneration enhanced by sustainable delivery of heparin from bilayer scaffolds carrying stem cells in a rat bladder partial cystectomy model

“…A variety of biomaterials have been used for bladder repair, including polymers [5], collagen [6,7], small intestine submucosa [7], cell sheets [8,9] and composite materials carrying cells [10][11][12]. Extracellular matrix (ECM) is a kind of biomaterial which retains the original tissue-specific components including collagen, glycolaminoglycan and related molecular growth factors.…”