Collagen and Gelatin in the Solid State

Yannas, Ioannis V.

doi:10.1080/15321797208068160

Cited by 256 publications

(103 citation statements)

References 106 publications

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“…Cross-linking was carried out in a vacuum oven (Fisher IsoTemp 201, Fisher Scientific, Boston, MA) at a temperature of 105 o C under a vacuum of 50 mTorr for 24 hours [1,25]. This process introduced covalent cross-links between the polypeptide chains of the collagen fibers without denaturing the collagen into gelatin [29].…”

Section: Cg Scaffold Crosslinkingmentioning

confidence: 99%

The effect of pore size on cell adhesion in collagen-GAG scaffolds

et al. 2005

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Section: Cg Scaffold Crosslinkingmentioning

confidence: 99%

The effect of pore size on cell adhesion in collagen-GAG scaffolds

et al. 2005

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“…Cross-linking was carried out under a vacuum (50mTorr) in a vacuum oven hours [15,17]; this process introduces covalent cross-links between the polypeptide chains of the collagen fibers without denaturing the collagen into gelatin [22]. Following cross-linking, the pore characteristics of the scaffolds were analyzed.…”

Section: Cg Scaffold Crosslinkingmentioning

confidence: 99%

Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds

O’Brien¹,

et al. 2004

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“…The resulting highly reactive oxygen species (ROS) can locally induce cell membrane damage (Figure 3(d)) [96][97][98]. Alternatively, pulsed UV (typically ns or ps pulses) may damage the cell membrane by molecular fragmentation after absorption of highly energetic UV photons [83,99]. Indeed, UV photons have sufficient energy to cause dissociation of many organic molecular bonds [100,101].…”

Section: Photochemical Pore Formationmentioning

confidence: 99%

Laser-assisted photoporation: fundamentals, technological advances and applications

Xiong

Samal

Demeester

et al. 2016

Advances in Physics: X

104

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Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-ofthe-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticlemediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.

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Collagen and Gelatin in the Solid State

Cited by 256 publications

References 106 publications

The effect of pore size on cell adhesion in collagen-GAG scaffolds

The effect of pore size on cell adhesion in collagen-GAG scaffolds

Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds

Laser-assisted photoporation: fundamentals, technological advances and applications

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