Collagen/chitosan based two-compartment and bi-functional dermal scaffolds for skin regeneration

Wang, Feng; Wang, Ming-Bo; She, Zhigang; Fan, Kang-Hsien; Xu, Cheng; Cui, Bin; Chen, Changsheng; Shi, Shengjun; Tan, Rongwei

doi:10.1016/j.msec.2015.03.013

Cited by 63 publications

(30 citation statements)

References 41 publications

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“…17 Wang et al prepared collagen/ chitosan-based scaffolds with VEGF and gentamicin encapsulated into poly(lactide-co-glycolic acid) (PLGA) microspheres and observed positive effects of these scaffolds on the adhesion and growth of mouse fibroblasts. 18 Collagen has also been widely applied in clinically used skin substitutes. For example, Integra (Integra LifeSciences, Plainsboro, NJ, USA), which is used for treating severe fullthickness burns, consists of a silicone layer on top of a porous matrix comprising a chemically cross-linked coprecipitate of bovine collagen and shark-derived chondroitin-6-sulfate.…”

Section: Introductionmentioning

confidence: 99%

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Bačáková¹,

Pajorová²,

Stránská³

et al. 2017

IJN

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Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide- co -glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.

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Section: Introductionmentioning

confidence: 99%

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Bačáková¹,

Pajorová²,

Stránská³

et al. 2017

IJN

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“…The study of gradients and stratification has been well documented for tissues like the muscle‐ligament‐bone complex (B. S. Lu, Subramony, Boushell, & Zhang, ; Merceron et al, ; Soo Kim et al, ; G. Yang et al, ), osteochondral tissue (Camarero‐Espinosa et al, ; Di Luca, Van Blitterswijk, & Moroni, ; Harley et al, ; Levingstone et al, ; Mosher et al, ; Spalazzi et al, ), or thin epithelial barriers such as the epidermis/dermis (Asano et al, ; Matsusaki et al, ; Ng et al, ; Wang et al, ), or gastrointestinal tissues (Nishiguchi et al, ; Pi et al, ). Most approaches are based on when and where cells are deposited on scaffolds to improve their chances of forming layers or gradients.…”

Section: Resultsmentioning

confidence: 99%

Dual‐chambered membrane bioreactor for coculture of stratified cell populations

Navarro

Swayambunathan

Janes

et al. 2019

Biotech & Bioengineering

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We have developed a dual-chambered bioreactor (DCB) that incorporates a membrane to study stratified 3D cell populations for skin tissue engineering. The DCB provides adjacent flow lines within a common chamber; the inclusion of the membrane regulates flow layering or mixing, which can be exploited to produce layers or gradients of cell populations in the scaffolds. Computational modeling and experimental assays were used to study the transport phenomena within the bioreactor. Molecular transport across the membrane was defined by a balance of convection and diffusion; the symmetry of the system was proven by its bulk convection stability, while the movement of molecules from one flow line to the other is governed by coupled convection-diffusion. This balance allowed the perfusion of two different fluids, with the membrane defining the mixing degree between the two.The bioreactor sustained two adjacent cell populations for 28 days, and was used to induce indirect adipogenic differentiation of mesenchymal stem cells due to molecular cross-talk between the populations. We successfully developed a platform that can study the dermis-hypodermis complex to address limitations in skin tissue engineering. Furthermore, the DCB can be used for other multilayered tissues or the study of communication pathways between cell populations.

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“…In the recent past, the use of various nanoparticle based microspheres, specifically PLGA, and natural polymers such as collagen or gelatin microspheres, has been reported for developing dermal or skin regeneration scaffolds for the effective delivery of drugs such as antibiotics or growth factors [177,178,179,180,181,182,183]. The size of microspheres in the scaffolds can be adjusted for the controlled release of proteins or drugs [177].…”

Section: Scaffolding Approaches and Different Types Of Scaffolds Imentioning

confidence: 99%

“…The size of microspheres in the scaffolds can be adjusted for the controlled release of proteins or drugs [177]. In a recent report, PLGA microsphere-based scaffolds were designed with a growth factor as well as gentamicin, and therefore effectively facilitated adhesion and proliferation of fibroblast cells with an antibacterial effect against Staphylococcus bacteria [183]. Also, mesenchymal stem cells-seeded microsphere scaffolds were successfully used as a skin construct and showed positive impact on cutaneous wound healing and sweat gland repair [184].…”

Section: Scaffolding Approaches and Different Types Of Scaffolds Imentioning

confidence: 99%

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Chaudhari

Vig

Baganizi

et al. 2016

IJMS

466

288

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Over centuries, the field of regenerative skin tissue engineering has had several advancements to facilitate faster wound healing and thereby restoration of skin. Skin tissue regeneration is mainly based on the use of suitable scaffold matrices. There are several scaffold types, such as porous, fibrous, microsphere, hydrogel, composite and acellular, etc., with discrete advantages and disadvantages. These scaffolds are either made up of highly biocompatible natural biomaterials, such as collagen, chitosan, etc., or synthetic materials, such as polycaprolactone (PCL), and poly-ethylene-glycol (PEG), etc. Composite scaffolds, which are a combination of natural or synthetic biomaterials, are highly biocompatible with improved tensile strength for effective skin tissue regeneration. Appropriate knowledge of the properties, advantages and disadvantages of various biomaterials and scaffolds will accelerate the production of suitable scaffolds for skin tissue regeneration applications. At the same time, emphasis on some of the leading challenges in the field of skin tissue engineering, such as cell interaction with scaffolds, faster cellular proliferation/differentiation, and vascularization of engineered tissues, is inevitable. In this review, we discuss various types of scaffolding approaches and biomaterials used in the field of skin tissue engineering and more importantly their future prospects in skin tissue regeneration efforts.

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Collagen/chitosan based two-compartment and bi-functional dermal scaffolds for skin regeneration

Cited by 63 publications

References 41 publications

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Dual‐chambered membrane bioreactor for coculture of stratified cell populations

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

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