Collagen/chitosan/hyaluronic acid – based injectable hydrogels for tissue engineering applications – design, physicochemical and biological characterization

Chitosan and collagen-based hydrogel composites were successfully prepared at chitosan:collagen volume ratio of 1:1, 1:3, 1:5, and 1:10. The structural, thermal, morphological, swelling, mechanical characteristics were investigated, and in vitro degradation testing was conducted. Tetraethyl orthosilicate (TEOS) was used as a crosslinking agent between the chitosan and collagen chains. The NH group of both chitosan and collagen can form crosslinks with the OH groups of TEOS. XRD reported that no significant peak of crystallinity was observed during the integration of collagen into the chitosan film. The surface of the composite was rougher than that of the collagen. The composites were thermally stable up to 200 °C. The glass transition temperature slightly decreased in the presence of collagen. The tensile strength significantly decreased, while the elongation at break significantly increased, when collagen was integrated into the chitosan film. The collagen conferred strong swelling and rapid degradation on the composite. The chitosan/collagen composite films developed in this study have potential applications as pharmaceutical materials.

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“…Phase separation occurred during the crosslinking of chitosan and collagen. This supported the findings of Gilarska et al [18].…”

Section: Physicochemical Properties Of Chitosan and Collagen-based Hysupporting

confidence: 92%

Integration of collagen into chitosan blend film composites: physicochemical property aspects for pharmaceutical materials

et al. 2020

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“…Attached proteins that a have hydrophilic nature increase surface wettability of the biomaterial. Thus, hydrophilic surfaces are considered as more biocompatible than hydrophobic ones [12,21,139,[156][157][158][159].…”

Section: Surface Wettabilitymentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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“…Gilarska et al focused on the obtaining ternary systems containing collagen/chitosan/hyaluronic acid. The obtained hydrogels can be widely used in bone regeneration procedures [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

et al. 2020

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In this work, synthesis and investigations on chitosan-based hydrogels modified with Aloe vera juice are presented. These materials were synthesized by UV radiation. Investigations involved analysis of chemical structure by FTIR spectroscopy, sorption properties in physiological liquids, strength properties by texture analyzer, surface topography by Atomic Force Microscopy (AFM technique), and in vitro cytotoxicity by MTT test using L929 murine fibroblasts. Particular attention was focused both on determining the impact of the amount and the molecular weight of the crosslinker used for the synthesis as well as on the introduced additive on the properties of hydrogels. It was proven that modified hydrogels exhibited higher swelling ability. Introduced additive affected the tensile strength of hydrogels—modified materials showed 23% higher elongation. The greater amount of the crosslinker used in the synthesis, the more compact the structure, leading to the lower elasticity and lower sorption of hydrogels was reported. Above 95%, murine fibroblasts remained viable after 24 h incubation with hydrogels. It indicates that tested materials did not exhibit cytotoxicity toward these lines. Additionally, materials with Aloe vera juice were characterized by lower surface roughness. Conducted investigations allowed us to state that such modified hydrogels may be considered as useful for biomedical purposes.

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Collagen/chitosan/hyaluronic acid – based injectable hydrogels for tissue engineering applications – design, physicochemical and biological characterization

Cited by 98 publications

References 50 publications

Integration of collagen into chitosan blend film composites: physicochemical property aspects for pharmaceutical materials

Integration of collagen into chitosan blend film composites: physicochemical property aspects for pharmaceutical materials

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

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