Collagen–DNA Complex

Abstract

Previously presented models of collagen-DNA (7) and collagen-siRNA (8) complexes point to a general description of delivery systems and indicate to what specific topology that system should be equipped with to effectively deliver the gene into the living body via in vivo and in vitro injection. We focused our interest on the nature of collagen-DNA complex structure and the molecular and environmental determinants of the self-association processes of collagen with the presence of DNA. In this aspect, the self-association of collagen-DNA complex offers an opportunity to characterize a unique system, which may be related to the general mechanisms of self-association of fiber macromolecules by water bridges. For characterizing the collagen-DNA interaction, we used FTIR-ATR, NMR, and AFM experiments done on a separate collagen film, DNA film, and on the peptide-DNA aqueous solution. We demonstrate that collagen-DNA spontaneously forms self-assembling complex systems in aqueous solution. Such self-association of the complex could be induced by electrostatic interactions between neutral collagen cylinders, having strong dipole moment, and negatively charged DNA cylinders. A final complex could be formed by hydrogen bonds between specified donor groups of collagen and phosphate acceptor groups of DNA. According to FTIR measurements, a collagen triple helix should not change global conformation during collagen-DNA complex formation.

Reducing Microbial and Human Contamination in DNA Extractions from Ancient Bones and Teeth

“…In addition, hydroxyapatite binds and inactivates nucleases (5). The interaction of DNA with collagen and its role in long-term preservation of DNA in ancient tissue is less well understood, but it has been hypothesized that DNA-collagen complexes, which form spontaneously in aqueous solutions in vitro, might play a protective role (3, 6). …”

Association between ancient bone preservation and dna yield: A multidisciplinary approach

“…For example, in aqueous solution the double helices of DNA form tightly packed complexes with up to 15 triple helices of collagen (Mrevlishvili and Svintradze, 2005;Svintradze et al, 2008). It is known that the DNA establishes strong bonds with the surrounding molecules.…”

Dielectric behaviour and conformational stability of collagen on interaction with DNA

“…The Rpn values for native collagen at different temperatures show minor differences from that of the collagen-DNA conjugate at different concentrations. The collagen-DNA conjugate formation was initiated due to the electrostatic interaction as well as hydrogen bonding between the donor group like CH 2 , CH (OH) of type I collagen and the acceptor phosphate group of DNA [6,29,32]. The hydrogen bonding are created directly either by collagen(CH)· · ·DNA(PO 4 ), or by collagen(CH)· · ·H 2 O· · ·DNA(PO 4 ).…”

“…If a system in which a gene vector is embedded in inactive biomaterial is administered into the living body, then the gene vector and bioactive protein can be protected from immunological and enzymatic attack. Thus, the release of the gene vector from the biomaterial and the gene expression period can be controlled [6][7][8]. Hence, it was found that DNA double helix and collagen triple helix will create a supramolecular self-assembly system in aqueous solution that will cause the rise in stability of DNA against the nucleases as well as the collagen developing the autoimmune reaction [9].…”

“…Recently, ATR-FTIR, AFM and NMR experiments were performed to study the mechanism of unusual collagen aggregation in the presence of DNA. It has been shown that collagen is protected from denaturation during the formation of collagen-DNA conjugate and could theoretically predict the possibilities of formation of hydrogen bonds between the peptide and DNA groups [6].…”