Collagen formation in vitro by established mammalian cell lines

Goldberg, Burton; Green, H.; Todaro, George J.

doi:10.1016/0014-4827(63)90025-9

Cited by 57 publications

(15 citation statements)

References 7 publications

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“…The findings of low but significant levels of prolyl hydroxylase activity in freshly isolated B and T cells are in contrast to previous reports (1,2,3,8). Very recently, prolyl hydroxylase and several other enzymes specifically associated with collagen biosynthesis were identified in human platelets, which are also of hematologic origin (28).…”

Section: Discussioncontrasting

confidence: 55%

“…This conclusion has been based on the presence of all of the following markers: prolyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2), the conversion of incorporated [14C]proline to [14Cjhydroxyproline, and the formation of collagenous protein (1)(2)(3)(4)(5)(6). To this list we recently added CRP (7), a protein that crossreacts with antibody to prolyl hydroxylase and is similar to one of the subunits of the enzyme.…”

mentioning

confidence: 99%

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Expression of collagen biosynthetic activities in lymphocytic cells.

Chen‐Kiang¹,

Cardinale²,

Udenfriend³

1978

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussioncontrasting

confidence: 55%

mentioning

confidence: 99%

Expression of collagen biosynthetic activities in lymphocytic cells.

Chen‐Kiang¹,

Cardinale²,

Udenfriend³

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…Replicate cultures were washed with phosphatebuffered saline (PBS) (pH 7.2) at 37°C and fixed in one per cent OsO4 in PBS at 0°C. Fixation, dehydration and embedding in Epon were performed in the original plastic culture plates as previously reported (1). Cultural morphology was excellently preserved throughout these procedures.…”

Section: Electron Microscopymentioning

confidence: 99%

An Analysis of Collagen Secretion by Established Mouse Fibroblast Lines

Goldberg

Green

1964

The Journal of Cell Biology

330

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In vitro synthesis of collagen by established mouse fibroblast lines has been examined by electron microscopy. During rapid growth (log phase), when collagen could not be detected in the cultures, the cells lacked a well developed granular ergastoplasm and Golgi system. Upon cessation of growth (stationary phase), collagen accumulated in the cultures and the cells demonstrated highly developed granular and smooth ergastoplasm. Collagen appeared to be synthesized in the rough-surfaced endoplasmic reticulum and to be transported as a soluble protein to the cell surface by vesicular elements of the agranular ergastoplasm. Fusion of the limiting membranes of these vesicles with the cell membrane permitted the dischargc of the soluble collagen into the extracellular space, where fibrils of two diameter distributions formed. The secretion of collagen is concluded to be of the mcrocrine type. Alternative theories of collagen secretion are discussed and the data for established lines compared with the results of other in vitro and in vivo studies of collagen fibrillogenesis.

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“…This tetraploid cell line has been well studied [45]; it has a doubling time of 13 h and is known to produce collagen [11]. All inoculations and transfers were in the form of trypsinized cell suspensions.…”

Section: Fig 4 14c-glucose Incorporation Into Diaphragmatic Glycogementioning

confidence: 99%

The case for an ?abnormal? insulin in diabetes mellitus

1968

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Summary. Understanding of diabetes in molecular terms has advanced very little. The possibility that a structural difference exists in the circulating and pancreatic insulin moiety of diabetics is supported by three lines of evidence obtained in the authors' laboratory. --Immunologically purified circulating insulin from diabetic subjects untreated with insulin was noted to be relatively resistant to degradation by a crude muscle insulinase preparation. The pancreatic insulin of five diabetic pancreases was found to have a decreased biological activity in its ability to enhance glycogen synthesis in vivo and in its capacity to stimulate RNA turnover in tissue culture. --The nature of this "abnormal insulin" and its hypothetical role in the physiopathology of diabetes are discussed in the light of the need for a specific definition of the precise molecular change. Exposg des arguments en faveur d'une ,,insuline anormale" dans le diab~teRdsumg. Pea de progr~s conduisant ~ la compr6hen-sion du diab~te en termes mol~culaires ont gt4 r6alisgs. La possibilit6 qu'il existe une modification dans la structure de l'insuline des diab~tiques, aussi bien circulante que pancr6atique, s'appuie sur trois arguments expgrimentaux obtenus au laboratoire des auteurs. --La purification imm,mochimique de l'insuline circulante de diabgtiques jeunes non tralt6s par

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Collagen formation in vitro by established mammalian cell lines

Cited by 57 publications

References 7 publications

Expression of collagen biosynthetic activities in lymphocytic cells.

Expression of collagen biosynthetic activities in lymphocytic cells.

An Analysis of Collagen Secretion by Established Mouse Fibroblast Lines

The case for an ?abnormal? insulin in diabetes mellitus

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