Collagen-GAG Substrate Enhances the Quality of Nerve Regeneration through Collagen Tubes up to Level of Autograft

Chamberlain, L.J.; Yannas, Ioannis V.; Hsu, Hu‐Ping; Strichartz, Gary R.; Spector, Myron

doi:10.1006/exnr.1998.6955

Cited by 206 publications

(169 citation statements)

References 48 publications

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“…It should be noted that previous studies have found that pore size reduces with T f as low as À808C. 10,29 However, the cooling rates used in these studies are greater (>18C=min) than the rate used in this study. This permits T f to influence both the shape of the ice crystals as they nucleate and the subsequent growth through diffusion.…”

Section: Novel Freeze-drying Methods Applied To Collagen-gag Scaffoldsmentioning

confidence: 57%

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Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Haugh

Murphy

O’Brien

2010

Tissue Engineering Part C: Methods

225

196

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• to copy, distribute, display, and perform the work.• to make derivative works. Under the following conditions:• Attribution -You must give the original author credit.• Non-Commercial -You may not use this work for commercial purposes.• The pore structure of three-dimensional scaffolds used in tissue engineering has been shown to significantly influence cellular activity. As the optimal pore size is dependant on the specifics of the tissue engineering application, the ability to alter the pore size over a wide range is essential for a particular scaffold to be suitable for multiple applications. With this in mind, the aim of this study was to develop methodologies to produce a range of collagen-glycosaminoglycan (CG) scaffolds with tailored mean pore sizes. The pore size of CG scaffolds is established during the freeze-drying fabrication process. In this study, freezing temperature was varied (À108C to À708C) and an annealing step was introduced to the process to determine their effects on pore size. Annealing is an additional step in the freeze-drying cycle that involves raising the temperature of the frozen suspension to increase the rate of ice crystal growth. The results show that the pore size of the scaffolds decreased as the freezing temperature was reduced. Additionally, the introduction of an annealing step during freeze-drying was found to result in a significant increase (40%) in pore size. Taken together, these results demonstrate that the methodologies developed in this study can be used to produce a range of CG scaffolds with mean pore sizes from 85 to 325 mm. This is a substantial improvement on the range of pore sizes that were possible to produce previously (96-150 mm). The methods developed in this study provide a basis for the investigation of the effects of pore size on both in vitro and in vivo performance and for the determination of the optimal pore structure for specific tissue engineering applications.

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Section: Novel Freeze-drying Methods Applied To Collagen-gag Scaffoldsmentioning

confidence: 57%

“…8,10,11 Using this technique, a suspension of collagen and chondroitin sulfate is frozen, trapping the CG fibers between growing ice crystals. This produces a continuous network of ice crystals surrounded by CG fibers.…”

mentioning

confidence: 99%

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Haugh

Murphy

O’Brien

2010

Tissue Engineering Part C: Methods

225

196

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“…2 PEG solution therapy combined with nerve tubes demonstrates a statistically significant improvement in foot fault asymmetry score at weeks 1, 2, and 3 (p=0.007, p=0.01, and p=0.006 two-tailed t test). N=8 for PEG group and N=7 for control group Nerve tubes were selected for use in comparison to microsuture because collagen conduits have demonstrated improved regeneration when utilized to repair nerve gaps [9,12,23,26,30]. For the purposes of this study, we used a collagen tube to prevent microsuture placement at the site of nerve repair and as a conduit for delivery of solutions.…”

Section: Discussionmentioning

confidence: 99%

Axonal Fusion via Conduit-Based Delivery of Hydrophilic Polymers

Sexton

Rodriguez-Feo

Boyer

et al. 2015

Hand (New York, N,Y.)

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Background Hydrophilic polymers have been shown to improve physiologic recovery following repair of transected nerves with microsutures. Our study was designed to combine hydrophilic polymer therapy with nerve tubes (NT) to enhance polymer delivery to the site of nerve injury. Methods Using a rat sciatic nerve injury model, a single transection injury was repaired in an end-to-end fashion with NT+polyethylene glycol (PEG) to NT alone. Compound action potentials (CAPs) were recorded before nerve transection and after repair. Behavioral testing was performed for 5 weeks. Results PEG therapy restored CAPS in all, but one, animals, while no CAPS were recorded in animals not receiving PEG. Behavioral nerve function was measured using the standardized functional assessment technique and foot fault asymmetry scores (FF). FF scores were improved for the PEG therapy groups on postoperative days 7, 14, and 21. However, after expected eventual axonal outgrowth, the benefit was less noticeable at days 28 and 35. Immunohistochemistry of the distal axon segments showed an increase number of sensory and motor axons in the NT+PEG group as compared to NT alone.Conclusion These data suggest that PEG delivery via a conduit may provide a simple, effective way to fuse severed axons and regain early nerve function. For proximal nerve injuries in large animals, recovery of axonal continuity could dramatically improve outcomes, even if fusion only occurs in a small percentage of axons.

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“…The biofunctionality and regenerative capacity of collagen can be significantly improved by the addition of glycosaminoglycans (GAGs) (Tierney et al, 2009). Scaffolds fabricated from copolymers of collagen and GAGs have been successfully applied in vitro and in vivo for skin, tendon, nerve, conjunctiva and more recently for bone and cartilage repair applications (Yannas and Burke, 1980, Louie et al, 1997, Chamberlain et al, 1998, Pieper et al, 2000, Hsu et al, 2000, Farrell et al, 2006, Lyons et al, 2010a.…”

Section: Introductionmentioning

confidence: 99%

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Matsiko

Levingstone

O’Brien

et al. 2012

Journal of the Mechanical Behavior of Biomedical Materials

142

102

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Collagen-GAG Substrate Enhances the Quality of Nerve Regeneration through Collagen Tubes up to Level of Autograft

Cited by 206 publications

References 48 publications

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Axonal Fusion via Conduit-Based Delivery of Hydrophilic Polymers

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

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