Collagen Gene Expression in Keloids: Analysis of Collagen Metabolism and Type I. III, IV, and V Procollagen mRNAs in Keloid Tissue and Keloid Fibroblast Cultures

Ala‐Kokko, Leena; Rintala, A; Savolainen, Eeva‐Riitta

doi:10.1111/1523-1747.ep12471056

Cited by 73 publications

(43 citation statements)

References 27 publications

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“…This study detected transcripts for three collagen members (collagen types I, III and VII). Transcripts for collagen types I and III are abundantly present in the alpaca skin, which is consistent with a previous report (Ala-Kokko et al, 1987) showing collagen types I and III are the main collagen members expressed in human skin (80% to 90% and 10% to 15% of the total collagens, respectively). Increased expression of SPARC is associated with collagen production, and lack of SPARC results in decreased collagen accumulation (Strandjord et al, 1999).…”

Section: Discussionsupporting

confidence: 92%

Gene expression profile in white alpaca (Vicugna pacos) skin

Fan

Dong

Cao

et al. 2011

Animal

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A cDNA library from white alpaca (Vicugna pacos) skin was constructed using SMART technology to investigate the global gene expression profile in alpaca skin and identify genes associated with physiology of alpaca skin and pigmentation. A total of 5359 high-quality EST (expressed sequence tag) sequences were generated by sequencing random cDNA clones from the library. Clustering analysis of sequences revealed a total of 3504 unique sequences including 739 contigs (assembled from 2594 ESTs) and 2765 singletons. BLAST analysis against GenBank nr database resulted in 1287 significant hits ( E-value , 10 210 ), of which 863 were annotated through gene ontology analysis. Transcripts for genes related to fleece quality, growth and coat color (e.g. collagen types I and III, troponin C2 and secreted protein acidic and rich in cysteine) were abundantly present in the library. Other genes, such as keratin family genes known to be involved in melanosome protein production, were also identified in the library. Members (KRT10, 14 and 15) of this gene family are evolutionarily conserved as revealed by a cross-species comparative analysis. This collection of ESTs provides a valuable resource for future research to understand the network of gene expression linked to physiology of alpaca skin and development of pigmentation.

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Section: Discussionsupporting

confidence: 92%

Gene expression profile in white alpaca (Vicugna pacos) skin

Fan

Dong

Cao

et al. 2011

Animal

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“…Knowledge-based directed therapy requires a detailed understanding of molecular mechanisms and the identification of cause and effect relationship, yet little is known regarding keloid pathogenesis. As previously reported, the expression of type I collagen proteins is increased in keloids [10,29]. Furthermore, changes in gene expression and growth factor kinetics due to passage and/or long-time culture have become an important problem in in vitro research using primary fibroblasts [30,31].…”

Section: Discussionmentioning

confidence: 98%

Comparative study of microRNA profiling in keloid fibroblast and annotation of differential expressed microRNAs

Li¹,

Bai²,

Liu³

et al. 2013

ABBS

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Keloids are tumor-like skin scars that grow as a result of the aberrant healing of skin injuries, with no effective treatment. The molecular mechanism underlying keloid pathogenesis is still largely unknown. In this study, we compared microRNA (miRNA) expression profiles between keloidderived fibroblasts and normal fibroblasts (including fetal and adult dermal fibroblasts) by miRNA microarray analysis. We found that the miRNA profiles in keloid-derived fibroblasts are different with those in normal fibroblasts. Nine miRNAs were differentially expressed, six of which were significantly up-regulated in keloid fibroblasts (KFs), including miR-152, miR-23b-3p, miR-31-5p, miR-320c, miR-30a-5p, and hsv1-miR-H7, and three of which were significantly down-regulated, including miR-4328, miR-145-5p, and miR-143-3p. Functional annotations of differentially expressed miRNA targets revealed that they were enriched in several signaling pathways important for scar wound healing. In conclusion, we demonstrate that the miRNA expression profile is altered in KFs compared with in fetal and adult dermal fibroblasts, and the expression profile may provide a useful clue for exploring the pathogenesis of keloids. miRNAs might partially contribute to the etiology of keloids by affecting several signaling pathways relevant to scar wound healing.

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“…All cultures (palmar and nonpalmar) showed upregulation of collagen I ( Figure 4A) and a-SMA ( Figure 4C), when TGFb1 was present in the media (lanes 2, 4,6,8). At the same time little effect was seen on the vimentin levels.…”

Section: Protein Expression In Fibroblasts From Keloid Formers and Nomentioning

confidence: 93%

Myofibroblast phenotype and apoptosis in keloid and palmar fibroblasts in vitro

Chipev¹,

Simman²,

Hatch³

et al. 2000

Cell Death Differ

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Keloid formation is a wound healing response, which fails to resolve and leads to formation of a raised collagen mass extending beyond the original wound margins. Keloids are typically excluded from palms and soles. Therefore we compared keloid and palmar fibroblasts in vitro using fibroblasts from nonaffected individuals as controls. Collagen I, a-smooth muscle actin and thrombospondin-1 were found at higher levels in keloid than in palmar fibroblasts. These differences were ameliorated by addition of TGFb1. The potential for resolution of the wound healing response was estimated analyzing apoptosis during serum starvation. Annexin V and TUNEL assays showed that palmar fibroblasts underwent faster apoptosis, than did the keloid fibroblasts, and started detaching. Addition of TGFb1 counteracted this effect. The weak expression of the myofibroblast phenotype and the advanced apoptosis of palmar fibroblasts suggest mechanisms for the exclusion of keloids from palmar sites.

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Collagen Gene Expression in Keloids: Analysis of Collagen Metabolism and Type I. III, IV, and V Procollagen mRNAs in Keloid Tissue and Keloid Fibroblast Cultures

Cited by 73 publications

References 27 publications

Gene expression profile in white alpaca (Vicugna pacos) skin

Gene expression profile in white alpaca (Vicugna pacos) skin

Comparative study of microRNA profiling in keloid fibroblast and annotation of differential expressed microRNAs

Myofibroblast phenotype and apoptosis in keloid and palmar fibroblasts in vitro

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