Collagen-I and fibronectin modified three-dimensional electrospun PLGA scaffolds for long-term in vitro maintenance of functional hepatocytes

Das, Prativa; DiVito, Michael D.; Wertheim, Jason A.; Tan, Lay Poh

doi:10.1016/j.msec.2020.110723

Cited by 37 publications

(26 citation statements)

References 34 publications

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“…The extracellular matrix (ECM) of the liver contains various proteins (e.g., collagen, fibronectin, and laminin). Our gene expression results demonstrate that the dual ECM (collagen and fibronectin) coating strategy can more closely mimic the ECM environments of liver and produce better hepatic functions than the single ECM coating, which is consistent with other reports [54,63].…”

Section: Cell-laden Ecm-coated Albumin-based Cryogels Promote Hepatic...supporting

confidence: 92%

An Engineered Protein-Based Building Block (Albumin Methacryloyl) for Fabrication of a 3D In Vitro Cryogel Model

Niu

Lin

Lee

2022

Gels

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Drug-induced liver injury (DILI) is a leading cause of attrition in drug development or withdrawal; current animal experiments and traditional 2D cell culture systems fail to precisely predict the liver toxicity of drug candidates. Hence, there is an urgent need for an alternative in vitro model that can mimic the liver microenvironments and accurately detect human-specific drug hepatotoxicity. Here, for the first time we propose the fabrication of an albumin methacryloyl cryogel platform inspired by the liver’s microarchitecture via emulating the mechanical properties and extracellular matrix (ECM) cues of liver. Engineered crosslinkable albumin methacryloyl is used as a protein-based building block for fabrication of albumin cryogel in vitro models that can have potential applications in 3D cell culture and drug screening. In this work, protein modification, cryogelation, and liver ECM coating were employed to engineer highly porous three-dimensional cryogels with high interconnectivity, liver-like stiffness, and liver ECM as artificial liver constructs. The resulting albumin-based cryogel in vitro model provided improved cell–cell and cell–material interactions and consequently displayed excellent liver functional gene expression, being conducive to detection of fialuridine (FIAU) hepatotoxicity.

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Section: Cell-laden Ecm-coated Albumin-based Cryogels Promote Hepatic...supporting

confidence: 92%

An Engineered Protein-Based Building Block (Albumin Methacryloyl) for Fabrication of a 3D In Vitro Cryogel Model

Niu

Lin

Lee

2022

Gels

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“…Conversely, natural polymers such as collagen and synthetic biodegradable materials have also been studied alone or in combination to generate cell-supporting and functional hepatic scaffolds or cell delivery systems [117][118][119][120][121][122]. However, these scaffolds could not provide cells with all the bioactive molecules required for their growth as compared with decellularized ECM, which is why synthetic materials were mixed with ECM powder or solubilized ECM to make them more biocompatible and functional.…”

Section: Bioengineered Liver Scaffolds and Their Transplantationmentioning

confidence: 99%

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

et al. 2021

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Purpose of Review To describe experimental liver injury models used in regenerative medicine, cell therapy strategies to repopulate damaged livers and the efficacy of liver bioengineering. Recent Findings Several animal models have been developed to study different liver conditions. Multiple strategies and modified protocols of cell delivery have been also reported. Furthermore, using bioengineered liver scaffolds has shown promising results that could help in generating a highly functional cell delivery system and/or a whole transplantable liver. Summary To optimize the most effective strategies for liver cell therapy, further studies are required to compare among the performed strategies in the literature and/or innovate a novel modifying technique to overcome the potential limitations. Coating of cells with polymers, decellularized scaffolds, or microbeads could be the most appropriate solution to improve cellular efficacy. Besides, overcoming the problems of liver bioengineering may offer a radical treatment for end-stage liver diseases.

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“…5 However natural polymers have the general advantages of being naturally biodegradable and non-toxic by products as well as biocompatible. 6,7 Among natural polymers, gelatin is a protein-based biopolymer which is supportive of cell growth, biodegradable and biocompatible and is widely used in the biomedical and food industries. 8 Gelatin is a collagen derivative and manufactured through acid/base hydrolysis of collagen present in bones and hides of bovine/pork.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and fabrication of gelatin-based elastomeric hydrogels through cosolvent-induced polymer restructuring

Panwar

Lee

et al. 2022

RSC Adv.

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Collagen-I and fibronectin modified three-dimensional electrospun PLGA scaffolds for long-term in vitro maintenance of functional hepatocytes

Cited by 37 publications

References 34 publications

An Engineered Protein-Based Building Block (Albumin Methacryloyl) for Fabrication of a 3D In Vitro Cryogel Model

An Engineered Protein-Based Building Block (Albumin Methacryloyl) for Fabrication of a 3D In Vitro Cryogel Model

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

Synthesis and fabrication of gelatin-based elastomeric hydrogels through cosolvent-induced polymer restructuring

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