Collagen‐immobilized patch for repairing small tympanic membrane perforations: In vitro and in vivo assays

Abstract

Tympanic membrane (TM) perforation is still one of the most common otology complications. New designs of biomaterials, and lately tissue-engineered composites and grafts, have thoroughly revolutionized the management of TM perforation. In this study, we examined a biologically modified collagen-immobilized polydimethyl siloxane patch to repair TM perforation. In vitro potential of the aforementioned patch as a scaffold to support fibroblast cell growth and adhesion was assessed. An in vivo assay of the patch for initiating repair of TM perforations also was investigated. In vitro assay showed that the patch has significantly increased cell adhesion and growth in comparison with unmodified ones (p < 0.05). In vivo study also showed an overall closure rate of TM perforation of 70% and an average gain of 15.75 ± 4.29 dB in air-bone gap. This study shows that the preliminary in vivo evaluation of a modified siloxane patch in humans had promising results and is comparable to existing biomaterial patches.

Microbial Flora of Cochlear Implants by Gene Pyrosequencing

“…Other materials have been used for patching up the perforation such as fat, paper, hyaluronan, and synthetic and bioengineered grafts. Myringoplasty by using synthesized membranes of collagen matrix or collagen coated layer has been tried (30,31). Collagen is of interest, as it is a compatible material, which is part of the modulating matrix of connective tissue that enhances cell adhesion and proliferation, especially fibroblasts (31).…”

Conceptos y aplicaciones de la ingeniería tisular en Otorrinolaringología

“…La timpanoplastia con fascia temporal o pericondrio tragal sigue siendo el tratamiento de elección, aunque normalmente con una morbilidad quirúrgica considerable. Por este motivo, se han hecho recientemente grandes esfuerzos a fin de encontrar biomateriales alternativos que permitan unas intervenciones más fáciles y eficaces 34,35 .…”

Biomimetic modification of silicone tubes using sodium nitrite–collagen immobilization accelerates endothelialization

“…4,6 On the other hand, covalent immobilization of collagen onto AAc-grafted materials, through chemical bonds between amino groups of collagen and carboxyl functional groups of AAc, enhances cell adhesion, proliferation, and differentiation. 3,4,6 However, collagen is highly thrombogenic and accelerates platelet aggregation in those areas of a material that are not fully covered by endothelial cells. 11 Correspondence to: G. Amoabediny; e-mail: amoabediny@ut.ac.ir Nitric oxide (NO) is an important inhibitor of platelet adhesion.…”

“…1 To overcome this limitation, several physicochemical techniques have been employed to improve the properties of silicone through surface modification. 2,3 A widely used surface modification method is plasma graft polymerization of acrylic acid (AAc), which introduces functional carboxyl groups on an inert silicone surface. [4][5][6][7] Nowadays, biomimetic coatings incorporating bioactive molecules that can be bound successfully to the carboxyl groups of AAc, such as collagen and gelatin, 4,6 insulin, 8 and anticoagulants such as thrombodulin, 9 albumin, 10 and heparin 9,11,12 are extensively used.…”