Collagen study and regulation of the de novo synthesis by IGF-I in hemocytes from the gastropod mollusc,Haliotis tuberculata

Serpentini, Antoine; Ghayor, Chafik; Poncet, Jean-Marc; Hébert, V.; Galéra, Philippe; Pujol, Jean-Pierre; Boucaud‐Camou, E.; Lebel, Jean-Marc

doi:10.1002/1097-010x(20000901)287:4<275::aid-jez2>3.0.co;2-8

Cited by 39 publications

(6 citation statements)

References 55 publications

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“…Insulin-like biological effects involve a variety of molluscan cell types, including those from the nervous ganglia, mantle, digestive gland, gonad and haemolymph [33]–[34]. Insulin-related peptides were also shown to stimulate protein synthesis in haemocytes of H. tuberculata [35]–[36]. Follistatin also known as activin-binding protein is the binding and bioneutralization of members of the TGF-β superfamily, with a particular focus on activin, a paracrine hormone.…”

Section: Resultsmentioning

confidence: 99%

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

et al. 2012

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Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosis is currently an active research field. However, studies were still limited and not deep enough because of the lack of genomic resources such as genome or transcriptome sequences. In the present research, de novo transcriptome sequencing was performed for Crassostrea angulata, the most economically important cultured oyster species in China, at eight early developmental stages using the 454 sequencing technology. A total of 555,215 reads were produced with an average length of 309 nucleotides that were then assembled into 10,462 contigs. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes recovered diverse biological functions and processes. Six unique sequences related to settlement, metamorphosis and growth were subsequently analyzed by real-time PCR. Given the lack of whole genome information for oysters, transcriptome and de novo analysis of C. angulata from the eight different developing phases will provide important and useful information on early development mechanism and help genetic breeding of shellfish.

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Section: Resultsmentioning

confidence: 99%

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

et al. 2012

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“…These cells are however known to play important functions in immunity (110). They can induce tissue repair, because of their ability to secrete proteins of the extracellular matrix, like collagens, proteoglycans, fibronectins, and growth factors like IGF (111). Furthermore, they have been shown to be involved in shell repair (24,112).…”

Section: A Critical View Of the Standard Modelmentioning

confidence: 99%

The formation and mineralization of mollusk shell

2012

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In the last years, the field of mollusk biomineralization has known a tremendous mutation. The most recent advances deal with the nanostructure of shell biominerals, and with the identification of several shell matrix proteins: on one hand, the complex hierarchical organization of shell biominerals has been deciphered in few models, like nacre. On the other hand, although proteins represent a minor shell component, they are the major macromolecules that control biocrystal synthesis. Until recently, the paradigm was to consider that this control occurs by two antagonist mechanisms: crystal nucleation and growth inhibition. Emerging models try to translate a more complex reality, illustrated by the huge variety of shell proteins, characterized so far. The primary structure of many of them is composed of different functional domains, some of which exhibit enzymatic activity, while others may be involved in cell signalling. Many of them have unknown functions. Today, the shell matrix appears as a whole system, which regulates protein-mineral, protein-protein, and epithelium-mineral interactions. These aspects should be taken in account for the future models of shell formation.

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“…and the freshwater mussel Dreissena polymorpha Pallas, 1771 (Parolini et al 2011) have yielded hemocytes that appear capable of longer-term survival under in vitro conditions. Contaminant-free isolation and maintenance of hemocyte primary cultures for 7–10 days have been attained for Haliotis tuberculata L., 1758 (Lebel et al 1996; Farcy et al 2007; Latire et al 2012) and H. midae L., 1758 (van der Merwe et al 2010), and 15 days for D. polymorpha , providing in vitro systems for testing effects of ecotoxin-exposure or other stressors on innate immune function (Galloway and Depledge 2001) or for investigating tissue repair processes (Serpentini et al 2000). Importantly, these cells can be cryopreserved with post-thaw viabilities of 96% and 71% after 2 and 6 days, respectively (Poncet and Lebel 2003).…”

Section: Molluscan Primary Cell Culturesmentioning

confidence: 99%

Molluscan cells in culture: primary cell cultures and cell lines

2013

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata Say, 1818 embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host-parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.

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Collagen study and regulation of the de novo synthesis by IGF-I in hemocytes from the gastropod mollusc,Haliotis tuberculata

Cited by 39 publications

References 55 publications

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

The formation and mineralization of mollusk shell

Molluscan cells in culture: primary cell cultures and cell lines

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