Collagen synthesis and mineralization in the early phase of distraction bone healing

Vauhkonen, Matti; Peltonen, Jari; Karaharju, Erkki; Aalto, Kari; Alitalo, I.

doi:10.1016/0169-6009(90)90260-m

Cited by 66 publications

(48 citation statements)

References 26 publications

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“…Delloye et al 1990, Aronson 1991. Peltonen et al 1992 (Vauhkonen et al 1990). When, after an initial period of neutral fixation.…”

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

“…Thereafter, remodeling of the distraction area occurs during several months (Monticelli et al 1981. Tajana et al 1989, Delloye et al 1990, Vauhkonen et al 1990. Aronson 1991.…”

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

“…a fibrous interzone is created in the center of the distraction gap. In this more or less avascular matrix fibroblast-like cells synthesize Type I collagen (Vauhkonen et al 1990, Peltonen et al 1992. which i sembled into bundles and then organized according to the tensile force (Tajana et al 1989, Aronson 1991, Peltonen et al 1992.…”

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

“…incubated at room temperature for 3 h and lyophiliyed. For the determination of the fibrillar collagen types, peptides from the proteins of the organic matrix were produced by cleavage with cyanogen bromide (CNBr) (Eastman Kodak Co., U.S.A.) (Epstein et al 1971, Vauhkonen et al 1990. The peptides obtained were analyzed on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli 1970).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…Organic material was relyophilized and weighed to obtain the organic dry weight. The amount of mineral was derived by subtracting the organic dry weight t'rom the total dry weight (Vauhkonen et al 1990). Protein was determined by the method of Lowry et al ( I95 I ) and the amount of collagen was calculated on the basis of hydroxyproline content of the samples (Kivirikko et al 1967).…”

Section: Biochemical Analysismentioning

confidence: 99%

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Early bone matrix formation during distraction: A biochemical study in sheep

Kallio

Vauhkonen

Peltonen

et al. 1994

SORT

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“…Delloye et al 1990, Aronson 1991. Peltonen et al 1992 (Vauhkonen et al 1990). When, after an initial period of neutral fixation.…”

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

“…Thereafter, remodeling of the distraction area occurs during several months (Monticelli et al 1981. Tajana et al 1989, Delloye et al 1990, Vauhkonen et al 1990. Aronson 1991.…”

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

Section: _ _ _ _ _ _~ ~_ _ _ _ _____mentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

See 3 more Smart Citations

Early bone matrix formation during distraction: A biochemical study in sheep

Kallio

Vauhkonen

Peltonen

et al. 1994

SORT

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Mouse Models of Bone Healing: Fracture, Marrow Ablation, and Distraction Osteogenesis

2015

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Three commonly used murine surgical models of bone healing (closed fracture with intramedullary fixation, distraction osteogenesis (DO), and marrow ablation by reaming) are presented. Detailed surgical protocols for each model are outlined. The nature of the regenerative processes and the types of research questions that may be addressed with these models are briefly outlined. The relative strengths and weaknesses of these models are compared to a number of other surgical models that are used to address similar research questions. Refer to our companion article for more detailed overview of the underlying biology of each model.

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Overview of Biological Mechanisms and Applications of Three Murine Models of Bone Repair: Closed Fracture with Intramedullary Fixation, Distraction Osteogenesis, and Marrow Ablation by Reaming

2015

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Fractures are one of the most common large-organ, traumatic injuries in humans, and osteoporosis-related fractures are the fastest growing health care problem of aging. Elective orthopedic surgeries of the bones and joints also represent some of most common forms of elective surgeries performed. Optimal repair of skeletal tissues is necessary for successful outcomes of these many different orthopedic surgical treatments. Research focused on post-natal skeletal repair is therefore of immense clinical importance and of particular relevance in situations in which bone tissue healing is compromised due to the extent of tissue trauma or specific medical co-morbidities. Three commonly used murine surgical models of bone healing, closed fracture with intramedullary fixation, distraction osteogenesis (DO), and marrow ablation by reaming are presented. The biological aspects of these models are contrasted and the types of research questions that may be addressed with these models are presented.

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Collagen synthesis and mineralization in the early phase of distraction bone healing

Cited by 66 publications

References 26 publications

Early bone matrix formation during distraction: A biochemical study in sheep

Early bone matrix formation during distraction: A biochemical study in sheep

Mouse Models of Bone Healing: Fracture, Marrow Ablation, and Distraction Osteogenesis

Overview of Biological Mechanisms and Applications of Three Murine Models of Bone Repair: Closed Fracture with Intramedullary Fixation, Distraction Osteogenesis, and Marrow Ablation by Reaming

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