Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva, Selestina; Kokol, Vanja

doi:10.5772/24118

Cited by 246 publications

(273 citation statements)

References 167 publications

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“…The results of in vitro biodegradation of decellularized goat-lung matrix showed that with the progression of days, the matrix gradually degrades, and after 60h, the matrix was completely degraded. This result confirms that collagenases (endopeptidase) helped in degrading the triple helix region of the collagen molecule present in the ECM [67,68].…”

Section: In Vitro Biodegradation Of Decellularized Goat-lung Matrixsupporting

confidence: 73%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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In this study, scaffold was fabricated from goat-lung of cadaver goat by decellularization method for its potential application in tissue engineering. The cellular components of goat-lung were removed by employing trypsin-SDS based method of decellularization. DNA quantification and DAPI staining verified the effective removal of native cells from the goat-lung. Verhoeff-Van Gieson (VVG) staining, H&E staining and Periodic-Acid Schiff (PAS) staining histologically assessed the composition of native and decellularized goat-lung matrix-demonstrating the presence of collagen, elastin and glycoproteins in the decellularized goat-lung matrix. Antibacterial activity of decellularized goat-lung matrix was evaluated against gram-negative (Escherichiacoli) and gram-positive (Staphylococcusaureus) bacteria, which shows the presence of naturally occurring bioactive peptides-exhibiting the antibacterial activity against E. coli for up to 9h and against S. aureus for up to 5h. The decellularized goat-lung matrix, as demonstrated by MTT assay, was found to be biocompatibile to L929 mouse fibroblasts, HepG2, human skin derived mesenchymal stem cells (hS-MSCs), osteoblasts and BMMSCs. All the above results confer the applicability of the decellularized goat-lung matrix as a potential scaffold for tissue engineering applications.

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Section: In Vitro Biodegradation Of Decellularized Goat-lung Matrixsupporting

confidence: 73%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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“…Chemical cross-linking of gelatin with GA involves the reaction of -amino groups of Lys (lysine) or Arg (arginine) of the polypeptide chains with aldehyde groups of the cross-linking agent by a Schiff base reaction [30]. Moreover, cross-linking is typically associated with a color change from white to pale yellow [12].…”

Section: Resultsmentioning

confidence: 99%

“…Rosellini et al [23] studied the effect of the presence of collagenase in the phosphate buffer saline (PBS) solution during in vitro degradation of gelatin scaffolds cross-linked with GA, and found that a mainly hydrolytic degradation process occurs due to solvation and depolymerization of the polymeric chains [24].…”

mentioning

confidence: 99%

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

et al. 2013

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The thermal and hydrolytic degradation of electrospun gelatin membranes cross-linked with glutaraldehyde in vapor phase has been studied. In vitro degradation of gelatin membranes was evaluated in phosphate buffer saline solution at 37 ºC. After 15 days under these conditions, a weight loss of 68 % was observed, attributed to solvation and depolymerization of the main polymeric chains. Thermal degradation kinetics of the gelatin raw material and as-spun electrospun membranes showed that the electrospinning processing conditions do not influence polymer degradation. However, for cross-linked samples a decrease in the activation energy was observed, associated with the effect of glutaraldehyde cross-linking reaction in the inter-and intra-molecular hydrogen bonds of the protein. It is also shown that the electrospinning process does not affect the formation of the helical structure of gelatin chains. Polymer Testing 32 (5), 995http://dx

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“…Therefore, it can be assumed that GA is a favourable crosslinking reagent for gelatine electrospun membranes, as it can efficiently cross-link protein amino groups. Chemical cross-linking of gelatine with GA involves the reaction of -amino groups of lysine or arginine of the polypeptide chain with aldehyde groups of the cross-linking agent by a Schiff base reaction [50]. Moreover, cross-linking is typically associated to a colour change from white to pale yellow [25].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

Padrão

Machado

Casal

et al. 2015

International Journal of Biological Macromolecules

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The increase of antibiotic resistant microorganisms urged the development and synthesis of novel antimicrobial biomaterials to be employed in a broad range of applications, ranging from food packaging to medical devices. This work describes the production and characterization of a protein-based electrospun fibrous membranes bearing antimicrobial properties. Its composition is exclusively comprised of proteins, with fish gelatine as structural matrix and bovine lactoferrin (bLF) as the active antimicrobial agent. The bLF bactericidal effect was determined against clinical isolates of Escherichia coli and Staphylococcus aureus through microdilution assays. Two distinctive methods were used to incorporate bLF into the fish gelatine nanofibres: (i) as a filler in the electrospinning formulation with concentrations of 2, 5 and 10 (wt%), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and (ii) through adsorption in a solution with 40mgmL -1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively. and 10 (%wt), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and ii) through adsorption in a solution with 40 mg mL -1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively.

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Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Cited by 246 publications

References 167 publications

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

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