Collagenase 1A2 (<i><scp>COL</scp>1A2</i>) gene A/C polymorphism in relation to severity of dental fluorosis

Escobar‐García, Diana María; Mejía-Saavedra, Jesús; Jarquin-Yañez, Lizet; Molina-Frechero, Nelly; Pozos‐Guillén, Amaury

doi:10.1111/cdoe.12201

Cited by 20 publications

(14 citation statements)

References 30 publications

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“…High levels of F, Al, As, Pb, and Cr were a risk factor for dental fluorosis, but not Se, Zn, Cu, B, Ca, and P, which was a protective factor for dental fluorosis [28]. ESR (Estrogen receptor) rs1256049 (G > A, RsaI) rs2234693 (A > C, XbaI) [101] AMBN (Ameloblastin) rs4694075 (C/T) [102] TFIP11 (Tuftelin interacting protein 11) rs5997096 (C/T) [102] TUFT1 (Tuftelin) rs4970957 (A/G) [102] DLX1 (Homeobox protein DLX-1) rs788173 (A/G) [103] DLX2 (Homeobox protein DLX-2) rs743605 (A/G) [103] TIMP1 (Metalloproteinase inhibitor 1) rs4898 (C/T) [103] Skeletal fluorosis FRZB1 (frizzled-related protein 1) rs2242070 (A/G) [104] VDR (Vitamin D receptor) rs2228570 (Fok I) [105] GSTP1 (Glutathione S-transferase pi 1) rs1695 (A/G) [106] PRL (Prolactin) rs1341239 [107] MMP-2 (Matrix metallopeptidase 2) rs2287074 (G/A) rs243865 [108] At present, some studies have been reported on the co-exposure of fluorine and arsenic (As) or fluorine and aluminum (Al). Both arsenic and fluoride are ubiquitous in the environment.…”

Section: Co-exposure With Other Elementmentioning

confidence: 99%

Progressive Research in the Molecular Mechanisms of Chronic Fluorosis

Shen¹,

Feng²,

Xia³

et al. 2019

Environmental Chemistry and Recent Pollution Control Approaches

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Long-term excessive intake of fluoride (F) leads to chronic fluorosis, resulting in dental fluorosis and skeletal fluorosis. Chronic exposure to high doses of fluoride can also cause damage to soft tissues, especially when it passes through the blood-brain, blood-testis, and blood-placenta barrier, causing damage to the corresponding tissues. Fluorosis has become a public health problem in some countries or regions around the world. Understanding the pathogenesis of fluorosis is very important. Although the exact mechanism of fluorosis has not been fully elucidated, various mechanisms of fluoride-induced toxicity have been proposed. In this chapter, we will introduce the research progress of the mechanism of fluorosis, focusing on dental fluorosis, skeletal fluorosis, nervous and reproductive system toxicity, and influential factors related to fluoride toxicity (i.e., genetic background, co-exposure with other element). In addition, the application of proteomics and metabolomics in the study of the pathogenesis of fluorosis is also introduced. Currently, there is still no specific treatment for fluorosis. However, since fluorosis is caused by excessive intake of fluoride, avoiding excessive fluoride intake is the critical measure to prevent the disease. In endemic regions, health education and supplement diet with vitamins C, D and E, and calcium and antioxidant compounds are important.

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Section: Co-exposure With Other Elementmentioning

confidence: 99%

Progressive Research in the Molecular Mechanisms of Chronic Fluorosis

Shen¹,

Feng²,

Xia³

et al. 2019

Environmental Chemistry and Recent Pollution Control Approaches

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“…Similarly, Dawson 5 and Huang et al, 4 concluded that some polymorphisms of the COL1A2 gene are involved in the development of dental fluorosis. However, Escobar-García et al, 6 and Pragya & Vandana 8 argue that there is no evidence of association between polymorphisms of the COL1A2 gene and dental fluorosis in populations exposed to high concentrations of fluoride. Therefore, other studies with larger samples and more sensitive techniques are necessary to corroborate this possible relationship.…”

Section: Referencesmentioning

confidence: 99%

Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis

2018

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Objective: To compare the gene expression levels of collagen type I alpha 2 (COL1A2) in children with and without dental fluorosis. Methods: Cross-sectional study involving 92 children between 5 and 12 years of age. Socio-demographic characteristics, the presence of dental fluorosis by means of the Thylstrup-Fejerskov index, and gene expression analysis of COL1A2 in peripheral blood samples by reverse transcription polymerase chain reaction (RT-PCR) assays, were described. For the descriptive analysis, measures of central tendency, dispersion and proportions were used. Differences between the groups (p<0.05) were established by the Student t-test. Results: Mean age was 8.6 (SD=1.9) years, 51.1% were female; 54 children were diagnosed with fluorosis and 38 without fluorosis; prevalence of dental fluorosis was 58.7% (95% CI: 48.4% -68.9%). Gene expression of COL1A2 was statistically significantly lower (p<0.05) in the participants with dental fluorosis. Conclusion: There are differences in the expression levels of the COL1A2 gene among the population under study. Therefore, COL1A2 may be potentially involved in the development of dental fluorosis.Keywords: dental fluorosis; dental enamel; gene expression; collagen. Conflict of interests:The authors declare no conflicts of interest. Acknowledgements:The authors would like to thank Daniela Garrido, David Franco, Leydi Mendoza, Carlos Leal, Ana Anaya and the COO-SALUD Foundation for their great contribution and support in health day sessions, during which samples were collected. The authors would also like to thank the community of El Pozón for their participation and interest in this research.

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“…Therefore, there is a need to explore the possibility of the potential role of a genetic involvement in occurrence of dental fluorosis in a population living in a fluorosis endemic area, which is prevalent in many countries including some areas of India. A recent cross-sectional study reported that the collagenase 1A2 (COL1A2) gene A/C polymorphism is involved in dental fluorosis in fluorosis-endemic areas [9]. This study included 80 children living in such an area and aimed to detect such a polymorphism in dental fluorosis cases.…”

Section: Introductionmentioning

confidence: 99%

No Association was Detected between Polymorphism of COL1A2 Genes and Occurrence of Dental Fluorosis Among the Subjects Living in a Fluorosis-Endemic Area of India

Chowdhury¹,

Khijmatgar²,

Mustak³

et al. 2021

DOBCR

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Objective: To determine any genetic association of COL1A2 polymorphism and the occurrence of dental fluorosis within an Indian human dental fluorosis population. Material and Methods: Fifty-six (56) subjects from two groups i.e. cases with dental fluorosis from the Pavagada population (n=29) and a control group (n=27) without fluorosis, were explored. The ages ranged between 15 and 76 years (mean 50.8 years) were included, and the male to female ratio was 70:30. The severity of dental fluorosis was graded using WHO’s Thylstrup-Fejerskov index (TF), and the concentration of fluoride was determined by a fluoride ion selective electrode (ISE). Genomic DNA was extracted using the standard phenol-chloroform method. The rs412777 and rs414408 polymorphism in COL1A2 were genotyped using the Sanger sequence method. Results: Genotype distributions for rs412777 within each group were: AA 41%, AC 51%, and CC 7% for dental fluorosis participants, and AA 56%, AC 46%, and CC 0% for the control participants. Conclusions: The rs412777 and rs414408 polymorphisms in the COL1A2gene showed no significant association between COL1A2 and the occurrence of dental fluorosis amongst this Indian population.

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Collagenase 1A2 (COL1A2) gene A/C polymorphism in relation to severity of dental fluorosis

Abstract: The presence of polymorphism in the COL1A2 gene was not associated with the severity of dental fluorosis.

Cited by 20 publications

References 30 publications

Progressive Research in the Molecular Mechanisms of Chronic Fluorosis

Progressive Research in the Molecular Mechanisms of Chronic Fluorosis

Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis

No Association was Detected between Polymorphism of COL1A2 Genes and Occurrence of Dental Fluorosis Among the Subjects Living in a Fluorosis-Endemic Area of India

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