Collagenolytic activity of Vibrio vulnificus: potential contribution to its invasiveness

Smith, Gillian; Merkel, Joseph R.

doi:10.1128/iai.35.3.1155-1156.1982

Cited by 89 publications

(30 citation statements)

References 11 publications

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“…A number of factors have been implicated as contributing to disease caused by V. vulnificus, such as iron availability in human serum for their survival (Wright et al 1981) ; the presence of a cytolysin that lyses red blood cells and is cytotoxic for Chinese hamster ovary (CHO) cells, enhances vascular permeability in guinea pig skin and is lethal for mice (Kreger and Lockwood 1981) ; proteases like collagenase (Smith and Merkel 1982) and elastase (Kothary and Kreger 1985) ; phospholipases and chondroitin-sulphatase (Oliver et Correspondence to: M. Landgraf, Faculdade de Ciências Farmacêuticas, USP, Av. Prof. Lineu Prestes 580, Bloco 14, São Paulo, Brazil, CEP 05508-900.…”

Section: Introductionmentioning

confidence: 99%

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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M . L . G A RC IA M OR EN O AN D M . LA ND G RA F. 1998. The virulence factors of Vibrio vulnificusare not yet well understood. So far, many hydrolytic enzymes have been implicated in the pathogenesis of this micro-organism. The present research was carried out in order to study the presence of some of these enzymes in 133 V. vulnificus strains isolated from 45 seafood samples. The results showed that 100% of these strains were positive for the production of lecithinase and lipase (Tween-80), 99·2% for caseinolytic protease, 96·9% for DNase, 65·4% for mucinase and 46·6% for elastase. None of the strains was positive for the production of collagenase and 96% were haemolytic against sheep blood cells. In relation to colony morphology on brain heart infusion (BHI) agar and nutrient agar, 59·4% of strains showed opaque morphology on BHI agar and 57·9% on nutrient agar, 10·5% presented translucent morphology on both agars and 30·1 and 31·6% of strains showed a mixture of opaque and translucent morphology on BHI agar and nutrient agar, respectively. None of the translucent colonies was virulent to mice. Therefore, opacity was a useful marker for potential virulence. Of 45 food samples contaminated with V. vulnificus, 29 (64·4%) presented strains lethal to adult mice.

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Section: Introductionmentioning

confidence: 99%

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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“…Many pathogenic bacteria are known to secrete exotoxins and other hydrolytic enzymes that constitute factors associated with their virulence. For example, Vibrio vulnificus produces extracellular proteinase that may be important in helping the bacterium invade tissues containing elastin and collagen (16). Some yeast species such as Candida albicans, Candida tropicalis and Candida parapsilosis secrete carboxyl proteinases when cultured in a medium with protein as a sole nitrogen source (6,8,11).…”

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confidence: 99%

Purification and Characterization of Secretory Proteinase of Candida albicans

Yamamoto

Nohara

Uchida

et al. 1992

Microbiology and Immunology

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Proteolytic activity of medically important yeasts was tested in both YCB-BSA agar and medium.All of 134 strains of Candida albicans, 13 of 18 strains of Candida tropicalis and 11 of 18 strains of Candida parapsilosis had this activity, while none of 52 Candida glabrata strains or of 11 Cryptococcus neoformans strains tested had proteolytic activity.Strains of C. albicans fell into five groups based on the level and time-course of in vitro proteinase productivity.Five strains randomly selected from each group were tested for pathogenicity in mice. The strain possessing the strongest pathogenicity was used to purify proteinase. The molecular weight of the proteinase was approximately 44,000 daltons and its isoelectric point was pH 4.2. Optimal pH of the proteinase was 3.2 and the enzyme was stable below pH 7.0 and lost its activity above pH 8.0 at 37 C in a 60-min incubation.The 23 amino acid sequence of the proteinase N-terminus was determined.

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“…For example, isolate I's 16S rRNA gene sequence is 99% identical to those of Vibrio crassostreae, which has been reported a pathogen of oysters (Faury et al, 2004) and Vibrio splendidus, which causes disease in turbot larvae. Other Vibrio and Bacillus species have also been reported to contain collagenase genes with potential roles in disease (Dreisbach & Merkel, 1978;Smith & Merkel, 1982;Mäkinen & Mäkinen, 1987;Lund & Granum, 1999).…”

Section: Resultsmentioning

confidence: 99%

“…(Yu & Lee, 1999;Vaitkevicius et al, 2008), have been linked to pathogenicity and are regarded as virulence factors in human disease. They can cause tissue destruction and thus facilitate the invasion of a pathogen into internal parts of host tissue (Smith & Merkel, 1982;Harrington, 1996). The presence of collagenolytic bacteria in the marine environment is more widely distributed than previously thought (Dreisbach & Merkel, 1978;Takeuchi et al, 1992;Thomas et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A polyphasic approach to the exploration of collagenolytic activity in the bacterial community associated with the marine sponge Cymbastela concentrica

Yung

Kjelleberg

Thomas

2011

FEMS Microbiology Letters

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Collagen is an important, extracellular structural protein for metazoans and provides a rich nutrient source for bacteria that possess collagen-degrading enzymes. In a symbiotic host system, collagen degradation could benefit the bacteria, but would be harmful for the eukaryotic host. Using a polyphasic approach, we investigated the presence of collagenolytic activity in the bacterial community hosted by the marine sponge Cymbastela concentrica. Functional screening for collagenase activity using metagenomic library clones (227 Mbp) and cultured isolates of sponge's bacterial community, as well as bioinformatic analysis of metagenomic shotgun-sequencing data (106,679 predicted genes) were used. The results show that the abundant members of the bacterial community contain very few genes encoding for collagenolytic enzymes, while some low-abundance sponge isolates possess collagenolytic activities. These findings indicate that collagen is not a preferred nutrient source for the majority of the members of the bacterial community associated with healthy C. concentrica, and that some low-abundance bacteria have collagenase activities that have the potential to harm the sponge through tissue degradation.

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Collagenolytic activity of Vibrio vulnificus: potential contribution to its invasiveness

Cited by 89 publications

References 11 publications

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Purification and Characterization of Secretory Proteinase of Candida albicans

A polyphasic approach to the exploration of collagenolytic activity in the bacterial community associated with the marine sponge Cymbastela concentrica

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