Purification and Characterization of Secretory Proteinase of <i>Candida albicans</i>

Yamamoto, Tetsuro; Nohara, Kumiko; Uchida, Katsuhisa; Yamaguchi, Hideyo

doi:10.1111/j.1348-0421.1992.tb02064.x

Cited by 40 publications

(26 citation statements)

References 19 publications

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“…However, there is a trend relating Sap production and site of isolation in that both vulvovaginal and skin isolates of C. parapsilosis exhibit higher in vitro Sap activity than blood isolates (39,58,62,65,297). This has significant implications for infection models.…”

Section: Secreted Enzymesmentioning

confidence: 95%

Candida parapsilosis, an Emerging Fungal Pathogen

2008

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SUMMARY Candida parapsilosis is an emerging major human pathogen that has dramatically increased in significance and prevalence over the past 2 decades, such that C. parapsilosis is now one of the leading causes of invasive candidal disease. Individuals at the highest risk for severe infection include neonates and patients in intensive care units. C. parapsilosis infections are especially associated with hyperalimentation solutions, prosthetic devices, and indwelling catheters, as well as the nosocomial spread of disease through the hands of health care workers. Factors involved in disease pathogenesis include the secretion of hydrolytic enzymes, adhesion to prosthetics, and biofilm formation. New molecular genetic tools are providing additional and much-needed information regarding C. parapsilosis virulence. The emerging information will provide a deeper understanding of C. parapsilosis pathogenesis and facilitate the development of new therapeutic approaches for treating C. parapsilosis infections.

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Section: Secreted Enzymesmentioning

confidence: 95%

Candida parapsilosis, an Emerging Fungal Pathogen

2008

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“…C. neoformans does not produce Sap (89), although initial studies by Staib et al (73) to detect proteinases in C. neoformans led to the discovery of Sap in C. albicans. In contrast, A. fumigatus and S. aureus have been reported to produce various proteolytic enzymes (30,34,44,57,58), but despite these reports, no significant ELISA inhibition by urine specimens from rabbits infected with these organisms was observed.…”

Section: Discussionmentioning

confidence: 99%

Competitive Binding Inhibition Enzyme-Linked Immunosorbent Assay That Uses the Secreted Aspartyl Proteinase ofCandida albicansas an Antigenic Marker for Diagnosis of Disseminated Candidiasis

Morrison

Hurst

Reiss

2003

Clin Vaccine Immunol

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The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as virulence factors associated with adherence and tissue invasion. The potential use of proteinases as markers of invasive candidiasis led us to develop a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) to detect Sap in clinical specimens. Daily serum and urine specimens were collected from rabbits that had been immunosuppressed with cyclophosphamide and cortisone acetate and infected intravenously with 10 7 C. albicans blastoconidia. Disseminated infection was confirmed by organ culture and histopathology. Although ELISA inhibition was observed when serum specimens from these rabbits were used, more significant inhibition, which correlated with disease progression, occurred when urine specimens were used. Urine collected as early as 1 day after infection resulted in significant ELISA inhibition (mean inhibition ؎ standard error [SE] compared with preinfection control urine, 15.7% ؎ 2.7% [P < 0.01]), and inhibition increased on days 2 through 5 (29.4% ؎ 4.8% to 44.5% ؎ 3.5% [P < 0.001]). Urine specimens from immunosuppressed rabbits infected intravenously with Candida tropicalis, Candida parapsilosis, Candida krusei, Cryptococcus neoformans, Aspergillus fumigatus, or Staphylococcus aureus were negative in the assay despite culture-proven dissemination. Nonimmunosuppressed rabbits receiving oral tetracycline and gentamicin treatment were given 2 ؋ 10 8 C. albicans blastoconidia orally or intraurethrally to establish colonization of the gastrointestinal tract or bladder, respectively, without systemic dissemination; urine specimens from these rabbits also gave negative ELISA results. Dissemination to the kidney and spleen occurred in one rabbit challenged by intragastric inoculation, and urine from this rabbit demonstrated significant inhibition in the ELISA (mean inhibition ؎ SE by day 3 after infection, 32.9% ؎ 2.7% [P < 0.001]). The overall test sensitivity was 83%, the specificity was 92%, the positive predictive value was 84%, the negative predictive value was 91%, and the efficiency was 89% (166 urine samples from 33 rabbits tested). The specificity, positive predictive value, and efficiency could be increased to 97, 95, and 92%, respectively, if at least two positive test results were required for a true positive designation. The ELISA was sensitive and specific for the detection of Sap in urine specimens from rabbits with disseminated C. albicans infection, discriminated between colonization and invasive disease, reflected disease progression and severity, and has the potential to be a noninvasive means to diagnose disseminated candidiasis.

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“…Entre as leveduras, diversas espécies estão associadas a processos industriais e/ou descritas como patógenas oportunistas. São microrganismos de relevância por serem ótimas produtoras de biocompostos e constituírem fonte de proteínas e vitaminas (Aoki et al, 1994;Kirsop & Kurtzman, 1888;Macdonald & Odds, 1980;Shimizu et al, 1987;Yamamoto et al, 1992).…”

Section: Methodsunclassified

Seleção de leveduras da Região Amazônica para produção de protease extracelular

Neves¹,

Porto

Teixeira³

2006

Acta Amaz.

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RESUMOO objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10 6 UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte. PALAVRAS-CHAVESLeveduras, Região Amazônica, Protease Screening of yeasts from Amazon Region for extracellular protease production ABSTRACT The objective of this work was to identify 50 yeasts isolates obtained from differents substracts from Amazon Region and to select one species of higher proteolytic activity. Among the 26 identified species it had predominance of Candida aquatica (12%) and Candida tropicalis (10%). The submerged fermentation was carried out in Malt Extract

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Purification and Characterization of Secretory Proteinase of Candida albicans

Cited by 40 publications

References 19 publications

Candida parapsilosis, an Emerging Fungal Pathogen

Candida parapsilosis, an Emerging Fungal Pathogen

Competitive Binding Inhibition Enzyme-Linked Immunosorbent Assay That Uses the Secreted Aspartyl Proteinase ofCandida albicansas an Antigenic Marker for Diagnosis of Disseminated Candidiasis

Seleção de leveduras da Região Amazônica para produção de protease extracelular

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