Collagens, stromal cell‐derived factor‐1α and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel

David, Laurent; Dulong, Virginie; Coquerel, Bérénice; Cerf, Didier Le; Cazin, L.; Lamacz, Marek; Vannier, Jean Pierre

doi:10.1111/j.1365-2184.2008.00515.x

Cited by 19 publications

(19 citation statements)

References 51 publications

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“…However, Matrigel is a mouse tumor extract that bears little resemblance to the composition of brain, which is substantially different from normal tissues [13]. Specific to GBM migration, several brain mimetic hydrogels, including hyaluronic acid, have been employed by ourselves [14] and others [15–18]. However, although hydrogels are fibrous materials at the micro/nano scale, these fibers are not usually oriented and therefore, do not adequately mimic the aligned topographical features of white matter tracts.…”

Section: Introductionmentioning

confidence: 99%

Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors

et al. 2013

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Glioblastoma multiforme (GBM), one of the deadliest forms of human cancer, is characterized by its high infiltration capacity, partially regulated by the neural extracellular matrix (ECM). A major limitation in developing effective treatments is the lack of in vitro models that mimic features of GBM migration highways. Ideally, these models would permit tunable control of mechanics and chemistry to allow the unique role of each of these components to be examined. To address this need, we developed aligned nanofiber biomaterials via core–shell electrospinning that permit systematic study of mechanical and chemical influences on cell adhesion and migration. These models mimic the topography of white matter tracts, a major GBM migration ‘highway’. To independently investigate the influence of chemistry and mechanics on GBM behaviors, nanofiber mechanics were modulated by using different polymers (i.e., gelatin, poly(ethersulfone), poly(dimethylsiloxane)) in the ‘core’ while employing a common poly(ε-caprolactone) (PCL) ‘shell’ to conserve surface chemistry. These materials revealed GBM sensitivity to nanofiber mechanics, with single cell morphology (Feret diameter), migration speed, focal adhesion kinase (FAK) and myosin light chain 2 (MLC2) expression all showing a strong dependence on nanofiber modulus. Similarly, modulating nanofiber chemistry using extracellular matrix molecules (i.e., hyaluronic acid (HA), collagen, and Matrigel) in the ‘shell’ material with a common PCL ‘core’ to conserve mechanical properties revealed GBM sensitivity to HA; specifically, a negative effect on migration. This system, which mimics the topographical features of white matter tracts, should allow further examination of the complex interplay of mechanics, chemistry, and topography in regulating brain tumor behaviors.

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Section: Introductionmentioning

confidence: 99%

Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors

et al. 2013

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“…In the recent years, scientists have set up HA-based scaffolds in combination with other molecules, such as a different type of collagens [230,234], the kappaelastin (HA-E), present in the basement membrane of blood vessels [235], gelatine, PEG [231], and chitosan [232]. Besides these natural extracellular matrix materials, which are expensive and potentially could transmit pathogens [236], several natural polymers have been also studied, such as chitosan-alginate scaffold [125], or alginate hydrogel functionalized with D-or L-aminoacids [237].…”

Section: D Tumoral Cns Cultures On Scaffoldsmentioning

confidence: 99%

“…Moreover, several other matrix compositions have been tested with the aim of better reproducing the in vivo microenvironment. Thus, invasion assays have been performed in HA-based hydrogel [230,[233][234][235]]. …”

Section: D Models For Testing Motility/invasion Of Cns Tumorsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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“…The porosity of this system was interesting because it allowed the exchange of oxygen, nutrients, and metabolites (Thomas, Nozik, Patel, Singh, & Vohra, ). Our model demonstrated its efficiency for anticancer drug sensitivity evaluation and its adaptability as it was tuned with other ECM components or soluble factors (Coquerel et al, ; David et al, ; David, Le Cerf, Cazin, Lamacz, & Vannier, ). Other cellular types have been successfully cultured in this HA‐hydrogel in so far as they did possess the CD44 receptor which is one of the main receptors for HA (Kassim et al, ; Lee & Spicer, ; Tammi, Day, & Turley, ).…”

Section: Introductionmentioning

confidence: 94%

Hyaluronan‐based hydrogels as versatile tumor‐like models: Tunable ECM and stiffness with genipin‐crosslinking

Bonnesœur

Morin-Grognet

Thoumire

et al. 2020

J Biomedical Materials Res

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Three‐dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo. We developed a tunable hyaluronan‐based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs). For that, we pre‐functionalized our hydrogels with an adhesive polypeptide (poly‐l‐lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP). Then, we thoroughly characterized our substrates before testing them with glioblastoma and breast cancer cells, and thereafter with endothelial cells. Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre‐functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent. While PLL‐treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness‐dependent manner. Contrary to type III collagen, type IV collagen pre‐treated hydrogels supported the proliferation of glioblastoma cells. The as‐desired HA‐based 3D tumor‐like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.

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Collagens, stromal cell‐derived factor‐1α and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel

Abstract: Furthermore, using basic fibroblast growth factor and stromal cell-derived factor-1alpha, we also show that this three-dimensional reticulate matrix may be considered as a valuable model to study chemokinetic and chemotactic potentials of factors present in tumour stroma.

Cited by 19 publications

References 51 publications

Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors

Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Hyaluronan‐based hydrogels as versatile tumor‐like models: Tunable ECM and stiffness with genipin‐crosslinking

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