Collateral effects of targeting the nucleus pulposus via a transpedicular or transannular surgical route: a combined X-ray, MRI, and histological long-term descriptive study in sheep

Abstract

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Lipid nanocapsules for intracellular delivery of microRNA: A first step towards intervertebral disc degeneration therapy

“…These in vivo results are particularly important before considering the implementation of veterinary (canine patients) or human clinical trials. The choice of the injection surgical route is a topic of active discussion by the scientific community, especially the choice between trans-annular and trans-pedicular surgical routes [37,57,58]. No degeneration process of the control IVDs was observed, thus confirming no negative impact from using the trans-annular surgical route in our study.…”

“…Three lumbar intervertebral discs (IVD, n = 25) were randomly assigned to one of the three conditions: in situ injection (180 µL) of blank LNC (n = 5), miR-155 LNC (n = 9), or sham condition (control; n = 14). Injection into the NP was performed percutaneously by a transannular approach under fluoroscopic guidance using a 22 G needle, as in the conventional posterolateral procedure [37].…”

Stem Cells and Intervertebral Disc Regeneration Overview—What They Can and Can't Do

“…17 Therefore, an alternative transpedicular route to access the NP without violating the AF has been proposed for the administration of therapeutic agents inside the IVD. 18 However, a recent study from Decante and colleagues 19 showed that both the transannular and the transpedicular approaches induced IDD as demonstrated by magnetic resonance imaging (MRI) and histological analysis in an ovine model. In addition, the transpedicular approach caused persistent endplate defects, with Schmorl-like herniations and osseous displacement within the NP.…”