Collection and Characterization of Mexican Guava (Psidium Guajava L.) Germplasm

Padilla-Ramírez, José Saúl; González-Gaona, Ernesto

doi:10.17660/actahortic.2010.849.4

Cited by 16 publications

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“…In 2007 and 2008, the Institute for Forestry, Agriculture and Livestock Research (INIFAP) carried out an extensive collection of guava sampling germplasm from commercial plantations, home gardens, and native environments in 11 of the 32 states in Mexico (Padilla‐Ramírez & González‐Gaona, 2010). A germplasm collection was established at INIFAP's research station in Zacatecas, Mexico (21°44.7′N, 102°58.0′W, 1508 masl) using either cuttings or seeds.…”

Section: Methodsmentioning

confidence: 99%

“…Materials in the plantation were arranged in rows separated by 3 m, with a 1.5 m distance between trees. Agronomic practices were as described in Padilla‐Ramírez and González‐Gaona (2010). Forty‐eight materials (Dataset S1) were used in this study to explore the genetic diversity of guava, which included 35 accessions collected in Mexico and 8 from other parts of the world, including Bolivia ( n = 1), Brasil (2), Colombia (1), Cuba (1), Honduras (1), India (1), and South Africa (1).…”

Section: Methodsmentioning

confidence: 99%

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Development of single nucleotide polymorphism markers and genetic diversity in guava (Psidium guajava L.)

Diaz‐Garcia

Padilla-Ramírez

2022

Plants People Planet

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Societal impact statement There are many understudied fruits of great economic and productive importance for regional agricultural hubs. Guava is an American fruit species with attractive nutritional and adaptability characteristics. However, in many countries like Mexico, its cultivation is still limited to small‐to‐medium size plantings and home gardens and depends on poorly controlled germplasm that diminishes productivity and expansion. Our study provides valuable insight to better understand guava diversity and generate high‐yielding, high‐quality, and better adapted materials. Supporting the study and development of understudied crops will provide us with more resources for facing climate change adversities and for diversifying cropping systems. Summary Guava (Psidium guajava L.) is a fruit crop species native to tropical and subtropical regions of America with great productive and economic potential due to its extensive environmental adaptability, nutritional value, and medicinal properties. However, the lack of molecular resources for accelerated breeding, limited knowledge about its evolutionary and domestication history, and unfavorable policies have limited its genetic improvement and broader adoption as a commercial fruit crop. Here, we present the first diversity study in guava employing genome‐wide single nucleotide polymorphisms (SNPs). Forty‐eight accessions collected from Mexico and other continents were examined with more than 6000 high‐quality SNP markers, which represents a marker density increase of ~30x compared with previous studies. Relationships between genetic groupings and geographic origins were not apparent in this diverse guava collection using principal component and structure analyses. Extensive germplasm exchange among guava‐producing regions and limited varietal control at commercial plantations might have contributed to ambiguities when defining the true origin and identities of existing germplasm materials. However, an analysis of domestication syndrome traits (fruit size and sugar) by wild or improved germplasm revealed several putative genomic regions under selection. Knowledge about germplasm origins and genetic relatedness, in conjunction with reliable molecular resources and better agronomic practices, is necessary to support breeding efforts and facilitate broader adoption of orphan crops such as guava, which have increasingly important roles in light of climate change adversities and in diversifying diets and food systems.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Development of single nucleotide polymorphism markers and genetic diversity in guava (Psidium guajava L.)

Diaz‐Garcia

Padilla-Ramírez

2022

Plants People Planet

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“…1) were analyzed. They were taken from a guava tree c.v. Calvillo S-XX1 [2], located in the Germplasm Bank from the Experimental Site "Los Cañones", belonging to the INIFAP, in the Huanusco municipality, state of Zacatecas, México (latitude 21°44′43.6 N; longitude 102°58′02.0 W).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Guava fruit has important nutritional properties like the high content of C vitamin of approximately 80-100 mg per gram of fruit [1]. Also, it is rich in tannins, tri-terpenes, flavonoids, saponins, lecithin, potassium, and soluble fiber [2]. In México, the guava fruit is one of the most important crops, and the most cultivated cv is the "media china"; which is characterized by its pale pink pulp color, intense sweet flavor, and significant commercial value due to its high demand on food and medicinal products [1].…”

Section: Introductionmentioning

confidence: 99%

Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit

Mejía-Mendoza

Garcidueñas-Piña

Padilla-Ramírez

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Guava fruit softening is a crucial process during ripening and this process involves a number of enzymes that modifies the cell wall. Two of the enzymes that regulate this process are (a) the β-1, 4-endoglucanase 17 (BEG) which hydrolyze β-1, 4 bonds from cellulose and hemicellulose, and (b) β-galactosidase (BGA) that hydrolyzes pectin chains. Bioinformatics and expression analysis information on these genes is limited in guava fruit. Results A fragment of a β-1, 4-endoglucanase 17 (PgE17), and another of a β-galactosidase (PgGa1) were identified. These sequences have a similarity of more than 85% with those reported in the NCBI database. In the guava genome, one homologous sequence was found for PgE17 in Chr 4 and two homologous to PgGa1: one in Chr 3 and the other one in Chr 6. Putative protein PgE17 contains part of the glyco_hydro_9 domain. Putative protein PgGa1 has a part of the glyco_hydro_35 domain. Phylogenetic analysis of PgE17 and PgGa1 revealed that both are highly conserved inside the Myrtaceae family. In silico expression analysis showed that both PgE17 and PgGa1 work in a coordinated way with other cell wall modifier enzymes. Expression of these genes was found in all the guava samples analyzed. However, the highest expression was found in the fruit in the breaking and ripe states. Conclusions A β-1, 4-endoglucanase 17, and β-galactosidase 1 sequences were identified. PgE17 and PgGa1 are expressed in all the plant tissues, and fruit ripening states. Although, the highest expression was on breaker and ripe states.

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“…The genetic diversity of guava (wild and cultivated) and the identification of divergent genotypes worldwide has been reported in studies based on morphological (Hernández-Delgado et al, 2007;Urdaneta et al, 2007;Santos et al, 2008;Lozano et al, 2009;Fernandes Santos et al, 2010;Padilla-Ramírez and González-Gaona, 2010), molecular, and biochemical data. Molecular and biochemical studies used dominant markers, such as random amplified polymorphic DNAs (RAPDs) (Chen et al, 2007;Pessanha et al, 2011) and amplified fragment length polymorphisms (AFLPs) (Rodríguez et al, 2004;Hernández-Delgado et al, 2007;Corrêa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Multivariate analysis in a genetic divergence study of Psidium guajava

et al. 2014

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ABSTRACT. The family Myrtaceae is widespread in the AtlanticForest and is well-represented in the Espírito Santo State in Brazil. In the genus Psidium of this family, guava (Psidium guajava L.) is the most economically important species. Guava is widely cultivated in tropical and subtropical countries; however, the widespread cultivation of only a small number of guava tree cultivars may cause the genetic vulnerability of this crop, making the search for promising genotypes in natural populations important for breeding programs and conservation. In this study, the genetic diversity of 66 guava trees sampled in the southern region of Espírito Santo and in Caparaó, MG, Brazil were evaluated. A total of 28 morphological descriptors (11 quantitative and 17 multicategorical) and 18 microsatellite markers were used. Principal component, discriminant and cluster analyses, descriptive analyses, and genetic diversity analyses using simple sequence repeats were performed. Discrimination of accessions using molecular markers resulted in clustering of genotypes of the same origin, which A.M. Nogueira et al. 10658©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 10657-10668 (2014) was not observed using morphological data. Genetic diversity was detected between and within the localities evaluated, regardless of the methodology used. Genetic differentiation among the populations using morphological and molecular data indicated the importance of the study area for species conservation, genetic erosion estimation, and exploitation in breeding programs.

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Collection and Characterization of Mexican Guava (Psidium Guajava L.) Germplasm

Cited by 16 publications

References 0 publications

Development of single nucleotide polymorphism markers and genetic diversity in guava (Psidium guajava L.)

Development of single nucleotide polymorphism markers and genetic diversity in guava (Psidium guajava L.)

Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit

Multivariate analysis in a genetic divergence study of Psidium guajava

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