Collection, Gross Characteristics and Freezing of Baboon Semen

Abstract

Consistently better results were obtained using Rings 7 and 10 with the probe placed 6 to 7 in. into the rectum. When Rings 1 to 3 were used in combination with other rings, the ejaculates were usually contaminated with urine.

Birth of offspring following artificial insemination in the common marmoset,Callithrix jacchus

“…Insemination with ejaculated sperm produced conception rates of 13 to 40% in rhesus monkeys [Czaja et al, 1975;Dede and Plentl, 1966]. Likewise, 4-21% in chimpanzees [Gould et al, 1985], 17% in baboons [Kraemer & Cruz, 1969], and 25% in mayotten lemurs [Brun & Rumpler, 1989]. However, the resorption rate was higher than expected in the study reported here, possibly due to environmental stress which can have an adverse effect on fertility [Johnson et al, 1991].…”

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

“…Successful freezing of spermatozoa has been reported for cynomolgus monkeys [15,16,60,100], rhesus monkeys [55,77], baboons (Papio anubis) [46], squirrel monkeys (Saimiri sciureus) [19], African green monkeys [90], chimpanzees (Pan troglodytes) [31], and gorillas (Gorilla gorilla gorilla) [38,49]. To freeze spermatozoa from cynomolgus monkeys, we prepared a semen-diluting medium, TTE (Tes, Tris, egg yolk base) [84].…”

In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

“…Acrosomal status has also been monitored but the ranking of freezing protocols remained unchanged (Tollner et al, 1990). The sperm penetration assay might provide a more rig- Roussel and Austin, 1967Roussel and Austin, 1967Roussel and Austin, 1967Roussel and Austin, 1967Leverage et al, 1972Cho and Honjo, 1973Cho et al, 1975Mahone and Dukelow, 1978Tollner et al, 1990Denis et al, 1976Cranfield et al, 1988Kraemer and Vera Cruz, 1969Roussel and Austin, 1967Gould and Styperek, 1989 freezer "Glycerol was used as cryoprotectant. Where multiple extenders were compared, only the most successful is included in the table. orous test of fertilizing ability (Gould and Styperek, 1989), but it is cumbersome and technically demanding.…”