Collection of hyperconcentrated platelets with Trima Accel

Abstract

Although TA is a suitable device for PLT collection at very high concentrations, improvements are desirable to further increase the productivity above its currently validated upper collect concentration limit.

Effects of immediate or delayed addition of platelet additive solution on the in vitro quality of apheresis platelets

“…The very low values for PLT P‐selectin expression for both kinds of products throughout storage are the most surprising finding in our study. In two former studies using exactly the same method for determination of P‐selectin expression, we had obtained also low values for P‐selectin expression for PLTs collected as HPSs using TA and stored in PAS‐IIIM 6,9 . The values in these studies, however, ranged notably higher with means of 6% up to 17% during 7‐day storage.…”

“…Among these, the increasing need for more plasma especially for fractionation purposes, and the reduction of the number of side effects associated with the transfusion of plasma, for example, transfusion‐associated lung injury, are of major importance 3‐5 . This growing interest in manufacturing plasma‐reduced PCs to be stored in PLT additive solution (PAS) motivates transfusion specialists to develop new PASs, but it also has impact on apheresis techniques to collect hyperconcentrated PLT suspensions (HPSs) of high quality with no signs of PLT activation 6,7 . Furthermore, to simplify and accelerate the manufacturing procedure of single‐donor PLT concentrates (SDPs), the automated addition of PAS by the apheresis device immediately after the collection has recently become available.…”

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

“…The hyperconcentration may have resulted in platelet damage, and therefore we evaluated the Trima system, which uses a different collection system and hyperconcentration method. 8,12,13 Unfortunately, there were no differences in poststorage platelet viability regardless of the Terumo BCT system that was used (Tables 1 and 3; Figure 1A-B).…”

“…12,13 For the Haemonetics studies, the platelets were elutriated with PAS instead of plasma during collection by sterile docking a Haemonetics bag (Effluent #692) containing PAS to the collection set. The operator would unclamp and, as appropriate, clamp the tubing to the #692 bag to permit surging with PAS.…”

Do diluents interfere with the determination of platelet count in platelet concentrates?

“…The PLT concentration in standard PLT concentrates (PCs) varies usually between 750 and 2000 × 10 9 PLTs/L 1 . For several reasons the collection of PLT components with concentrations above 3000 × 10 9 PLTs/L, so‐called hyperconcentrated PLT concentrates (HCPs), is becoming more and more popular 2 . Therefore, dilution of the sample of PCs may be necessary to reach the range of linearity of the particular analyzer.…”