Environmental problems are one of the concerns of the world community. Environmental problems can be overcome if people have literacy toward the environment. The environmental literacy can be influenced by a variety of demographic variables. The purpose of this study was to describe the effect of gender on students' environmental literacy. This study is an ex post facto research. The population of this study was the twelfthgrade students majoring in Mathematics and Natural Sciences at Public High School 1 Melaya Jembrana Bali Indonesia. The number of samples in this study was 140 students consisting of 54 male and 86 female students. The instrument used in this study was an environmental literacy inventory consisting of five dimensions, including knowledge, attention, sensitivity, attitudes, and behavior toward the environment. The environmental literacy inventory was created in Google forms and distributed to students online. Students' environmental literacy data were analyzed using the independent sample t-test. The results showed that the environmental literacy of male and female students is still low category and there was no difference in environmental literacy between male and female students.