Colloid Au-enhanced DNA immobilization for the electrochemical detection of sequence-specific DNA

Cai, Hong; Xu, Chun; He, Pingang; Fang, Yuzhi

doi:10.1016/s0022-0728(01)00548-4

Cited by 237 publications

(112 citation statements)

References 40 publications

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“…An additional type of biomolecules, DNA, can also be immobilized with nanoparticles, and used for the creation of electrochemical DNA sensors. In command to immobilize DNA onto the surfaces of nanoparticles, the DNA strands are frequently modified with meticulous functional groups that can work together powerfully with convinced nanoparticles [84].…”

Section: Nanoparticles In Electrochemical Sensors and Biosensorsmentioning

confidence: 99%

Biogenic Synthesis of Nanoparticles and Potential Applications: An Eco- Friendly Approach

Ingale¹

2013

J Nanomedic Nanotechnol

253

114

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The uprising in materials science has been hosted by previous few decades. There has been a substantial research interest in the area of using particulate systems to accomplish various approaches. The conception or synthesis of material with nanometer-scale precision (nanoparticles), by means of material science, is nanotechnology. Nanoparticles are defined as particulate dispersal or solid particles, with a size in the range of 1-100 nm.This review intends to present biosynthesis and application of nanoparticles in minutiae. Here, we discussed different synthesis methods, i.e. chemical, physical and biogenic synthesis of nanoparticles. The potential come together between nanotechnology and biological science is enormous. Realistic biologics depends on units that have nanoscale dimensions (proteins, viruses, molecular motors, extra cellular matrix). Our major dictum is to focus the various facet of synthesis of nanoparticles, characterization, and its imperative application by giving accent on biogenic synthesis of nanoparticles.

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Section: Nanoparticles In Electrochemical Sensors and Biosensorsmentioning

confidence: 99%

Biogenic Synthesis of Nanoparticles and Potential Applications: An Eco- Friendly Approach

Ingale¹

2013

J Nanomedic Nanotechnol

253

114

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“…Some other functional groups are also investigated. Fang and co-workers [96] immobilized an oligonucleotide with a mercaptohexyl-group at the 5'-phosphate end onto the 16 nm diameter GNPs, which were self-assembled on a cystamine-modified gold electrode. The saturated immobilization quantities of single-strand DNA on the modified electrode were about 10 times larger than that of a bare gold electrode.…”

Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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“… Mercapto-saline coating of a platinum electrode were prepared by addition of Mercapto-saline to a mixture of acetate buffer and acetone which were later post baked, incubated with iodoacetic acid and activated with water-soluble carbodiimidies for the immobilization of DNA 40 .  The clean gold electrode soaked in cysteamine (monolayer of cysteamine/gold electrode), later dipped in gold colloid solution was able to immobilize ss-DNA on the colloidal Au-modified electrode forming self assembled (-S-(CH 2 ) 2 -NH-Au-ss-DNA) pattern 41 . Hollow gold nanospheres have also been implemented for the modification of electrode surface via a 1,6-hexane dithiol liking agent to fabricate electrochemical DNA biosensor with the hybridization detection limit up to 1pM 42 .…”

Section: Strategies Used For the Immobilization Of Dna Over Working/mmentioning

confidence: 99%

Development of Electrochemical DNA Biosensors-A Review

2015

Chem Sci Trans

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Electrochemical DNA biosensors are used widely due to its sensitivity, accessibility and accuracy. This electrochemical era in DNA biosensors took almost three decades for its development and application. Purine bases in the DNA namely guanine and adenine oxidizes at the working electrode during application of a positive potential. This property of DNA has been utilized in electrochemical DNA biosensors in various fields from the clinical diagnostics, biomedical, forensic applications till environmental studies. With the use of nanotechnology, various nanomaterials have been utilized over the past years in order to increase the sensitivity of the biosensor. A clear understanding on the DNA immobilization strategy is required for sensor preparation for its various applications. A detailed account on the initial developments till current scenario in electrochemical DNA biosensors have been provided in order to understand the basics of electrochemical DNA biosensor and its application in developing a DNA biosensor.

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Colloid Au-enhanced DNA immobilization for the electrochemical detection of sequence-specific DNA

Cited by 237 publications

References 40 publications

Biogenic Synthesis of Nanoparticles and Potential Applications: An Eco- Friendly Approach

Biogenic Synthesis of Nanoparticles and Potential Applications: An Eco- Friendly Approach

Gold nanoparticle-based biosensors

Development of Electrochemical DNA Biosensors-A Review

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