Colloidal gold-based immunochromatographic assay for detection of ricin

Co-detection: Ultra-reliable nanoparticle-based electrical detection of biomolecules in the presence of large background interference

“…1d). Even though, silver-enhancement technique has been used for conductometric DNA arrays and immunoasssays [13][14][15][16][17] , it has not been yet extended to implement logic-functions as is reported in this work. In the case of the soft-OR gate the conductive bridge between the electrodes is formed when either one of the analytes (rabbit IgG or mouse IgG) is present in the sample, assuming that the density of antibody probes is large enough.…”

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

“…An ICA was developed for the detection of anti-CHIKV capsid protein IgM using the method described previously (Chiao et al, 2004;Kim et al, 2007;Shyu et al, 2002). In brief, colloidal gold (40 nm in diameter, British Biocell International, UK) was used for the conjugation of anti-human IgM.…”

Development and evaluation of realistic microbioassays in freely suspended droplets on a chip

“…These advantages make them suitable for analysis of biotoxin agents and forensic samples of microscopic volumes and low concentrations. Assays based on other antibodies for ricin with higher sensitivity have been reported previously [28][29][30] . However, these assays use larger sample volumes in comparison to the droplet based microbioassay.…”

“…The experiment was then conducted by the same protocol as for GOAgg microbioassays. This experiment also allowed us to evaluate the performance of the droplet assays to the one of the standard DOD hand held assays ͑HHA͒ operating on immunochromatographic principles 1,27,28 . The ricin droplet microbioassays showed minimum gold nanoparticles collection for 1.0 g / mL concentration at 30 min incubation time ͑Fig.…”