Colloidal gold probe based rapid immunochromatographic strip assay for cortisol

Nara, Seema; Tripathi, Vineeta; Singh, Harpal; Shrivastav, Tulsidas G.

doi:10.1016/j.aca.2010.09.041

Cited by 51 publications

(26 citation statements)

References 18 publications

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“…The synthesized GNPs displayed zeta potential of −55.6 mV, confirming their stability as particles with zeta potentials more than +30 mV and more negative than −30 mV are normally considered stable (Nara et al . ).…”

Section: Resultsmentioning

confidence: 97%

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lata

Sharma

Naik

et al. 2015

Journal of Food Quality

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A rapid and semiquantitative lateral flow assay has been developed for detection of cephalexin (CFX) in milk. The assay is based on a competitive format using polyclonal antibodies. Antibodies were raised in rabbits against CFX-keyhole limpet hemocyanin. The specificity of antibodies was ascertained by direct enzyme-linked immunosorbent assay. The laboratory prepared gold nanoparticles coupled to anti-CFX antibodies, which competed for free CFX in sample and CFX-bovine serum albumin conjugate on the test line of a lateral flow strip. The control line of the lateral flow strip consisted of species-specific antibody. The assay was validated with spiked milk samples and involved application of skim milk to the adsorbent pad followed by visualization of red color on test and control lines within 8 min. The lower detection limit of CFX was 30 ppb in milk, which is lower than the Codex prescribed maximum residue limit. The storage stability of the prepared strip was also established. PRACTICAL APPLICATIONSA simple method for the detection of cephalexin in milk using lateral flow assay has been described. The applicability of polyclonal antibodies in the development of lateral flow assay has been demonstrated. The method is sensitive to detect 30 ppb cephalexin in milk, which is lower than the Codex prescribed maximum residue limit of 100 ppb. This method can be used for preliminary screening of milk samples for the presence of cephalexin residues in milk.

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Section: Resultsmentioning

confidence: 97%

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lata

Sharma

Naik

et al. 2015

Journal of Food Quality

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“…Other unique properties are the high chemical stability, large specific area, easy synthesis, low cost and easy preparation steps. These properties make the analysis time short and provide reliable analysis on-site [2,[35][36][37][38].…”

Section: Properties Of Aunpsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

503

315

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“…After the addition of NaCl, we measured the optical density (OD) at 520 nm, 580 nm, and 600 nm. We used the ratio of the OD at 520 nm to that at 580 nm to assess stability and the ratio of the OD at 600 nm to that at 520 nm to assess polydispersity (20)(21)(22). We found the highest stability and lowest polydispersity when colloidal gold was conjugated to both anti-human IgG and IgA at pH 8.0 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To assess stability of the conjugate and to define the optimum pH and minimum concentration of antibody required for conjugating the colloidal gold, we used an aggregation assay (20)(21)(22). Specifically, we added 10% NaCl to the gold-protein suspension, incubated it for 10 min, and then assessed stability and polydispersity by measuring the absorbance at 520 nm, 580 nm, and 600 nm (20)(21)(22).…”

Section: Ethics Statementmentioning

confidence: 99%

Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever

Khan¹,

Sayeed

Sultana³

et al. 2016

Clin Vaccine Immunol

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e Enteric fever is a systemic infection caused by typhoidal strains of Salmonella enterica and is a significant cause of mortality and morbidity in many parts of the world, especially in resource-limited areas. Unfortunately, currently available diagnostic tests for enteric fever lack sensitivity and/or specificity. No true clinically practical gold standard for diagnosing patients with enteric fever exists. Unfortunately, microbiologic culturing of blood is only 30 to 70% sensitive although 100% specific. Here, we report the development of a lateral-flow immunochromatographic dipstick assay based on the detection of Salmonella enterica serovar Typhi (S. Typhi) lipopolysaccharide (LPS)-specific IgG in lymphocyte culture secretion. We tested the assay using samples from 142 clinically suspected enteric fever patients, 28 healthy individuals residing in a zone where enteric fever is endemic, and 35 patients with other febrile illnesses. In our analysis, the dipstick detected all blood culture-confirmed S. Typhi cases (48/48) and 5 of 6 Salmonella enterica serovar Paratyphi A blood cultured-confirmed cases. The test was negative in all 35 individuals febrile with other illnesses and all 28 healthy controls from the zone of endemicity. The test was positive in 19 of 88 individuals with suspected enteric fever but with negative blood cultures. Thus, the dipstick had a sensitivity of 98% compared to blood culture results and a specificity that ranged from 78 to 100% (95% confidence interval [CI], 70 to 100%), depending on the definition of a true negative. These results suggest that this dipstick assay can be very useful for the detection of enteric fever patients especially in regions of endemicity.

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Colloidal gold probe based rapid immunochromatographic strip assay for cortisol

Cited by 51 publications

References 18 publications

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lateral flow assays: Principles, designs and labels

Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever

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