Colloidal Textile Dye-Based Dipstick Immunoassay for the Detection of Nuclear Polyhedrosis Virus (BmNPV) of Silkworm, Bombyx mori L.

Nataraju, B.; Sivaprasad, V.; Datta, R. Κ.; Gupta, Seema; Shamim, M

doi:10.1006/jipa.1994.1026

Cited by 9 publications

(3 citation statements)

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“…The present most common methodologies for BmNPV detection include microscopy, in vivo assays and nucleic acid based analysis (Joshi and Raja, 2016). Other techniques that have been developed to detect BmNPV include Enzyme-Link Immunosorbent Assay (ELISA) (Vanapruk et al, 1992), DNA hybridization (Attathom et al, 1994), colloidal textile dye-based dipstick immunoassay (Nataraju et al, 1994), protein-A linked monoclonal antibody latex agglutination test (PALMAL) (Shamim et al, 1995) and viral DNA transfection (Martinex-Zubiaur et al, 2016). The implementation of nucleic acid based analyses by Polymerase-Chain Reaction (PCR) and sequencing for BmNPV detection can be complicated by external factors.…”

Section: Bmnpv Detection Techniquesmentioning

confidence: 99%

Bombyx mori nucleopolyhedrovirus (BmBPV): Its impact on silkworm rearing and management strategies

Gani

Chouhan

Lal

et al. 2018

Journal of Biological Control

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The mulberry silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae) is infected with a baculovirus, Bombyx mori nucleopolyhedrovirus (BmNPV) that causes grasserie disease in silkworm and major economic losses to the silk industry. In India, >50 % of silk cocoon crop losses are attributed to BmNPV infection. Presently, there are no specific preventive measures for the occurrence and spread of BmNPV infection other than sanitized rearing methods, the only commercial practice today is to discard large stocks of worms in case of infection. Although diagnostic kits for detection of BmNPV have been developed, they are not extensively used on a commercial scale and subsequently, they fail to provide the indispensable and timely advantages desired for early disease intervention. The best emerging technology is the use of antibody-based biosensors and lateral flow assays, which have high specificity, sensitivity and the option of "onsite" pathogen detection. The use of disease resistant silkworm breeds or the utilization of inherent resistance in silkworm would be the most economical and effective way to prevent the occurrence of grasserie disease. Further, there is a great need to comprehensively analyze the host genes response to BmNPV infection and its functional analysis to prevent virus replication and its horizontal transmission.

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Section: Bmnpv Detection Techniquesmentioning

confidence: 99%

Bombyx mori nucleopolyhedrovirus (BmBPV): Its impact on silkworm rearing and management strategies

Gani

Chouhan

Lal

et al. 2018

Journal of Biological Control

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“…Dissection of diseased specimens was done to study the anatomical changes: size, shape and colour of organs after disease attack. From the diseased samples the viruses vizBmNPV, BmIFV and BmDNV1 were purified and isolated as per the standard methods (Nataraju et al, 1994;Sivaprasad et al, 2003;O'Reilly et al, 1992;Palhan and Gopinathan, 1996;Luo et al, 2010;Vootla et al, 2013). Preliminary BmNPV identification was done by light microscopy by spreading smears from infected larvae thinly across a microscope slide followed by Giemsa staining and then examined through a phase-contrast microscope at 1000X magnification under oil immersion (Gupta et al, 2013).…”

Section: Sampling and Virus Analysismentioning

confidence: 99%

Prevalence and molecular characterization of viruses causing diseases in Bombyx mori L. (Lepidoptera: Bombycidae) from different climatic regions of India

Gani

Josepha

Satish

et al. 2018

Journal of Biological Control

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A number of viruses are known to cause the disease in mulberry silkworm, Bombyx mori L. and cause significant cocoon crop loss to the farmers. The study has been undertaken to assess the prevalence of silkworm viral diseases caused by B. Mori Nucleopolyhedrovirus (BmNPV), B. mori Infectious Flacherie Virus (BmIFV) and B. mori Densovirus1 (BmDNV1) through external symptoms and molecular characterization from different climatic regions of India. During the intensive exploratory surveys in 2017, silkworm larvae with typical symptoms of BmNPV, BmIFV and BmDNV1 were collected across seven provinces from North India [Kashmir (temperate), Jammu, Ghumarwin in Himachal Pradesh, Dehradun in Uttarakhand (subtropical) and South India [Karnataka, Andhra Pradesh and Tamil Nadu (tropical)]. Dissection of diseased specimens confirmed the presence of virus through anatomical changes viz, size, shape and colour of organs after disease attack. The viruses were isolated and identified through PCR amplification of highly conserved genes. The results reveal that BmNPV, BmIFV and BmDNV1 are evenly prevalent across India. The infection percentage of BmNPV, BmIFV and BmDNV1 in North India (11.41 ± 1.21, 7.81 ± 0.67 and 7.40 ± 0.61) was significantly higher than South India (1.61 ± 0.17, 1.52 ± 0.14 and 1.62 ± 0.14), respectively. The highest prevalence of these viruses was observed from subtropical followed by temperate and tropical climate. The knowledge of the prevalence of these viruses in India and their synergism with bacteria and influence of other possible factors is important for preventing cocoon crop losses caused by viral diseases in India.

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“…Earlier, techniques have been developed to detect this viral disease, such as the enzyme-link immunosorbent assay (ELISA) (Vanapruk et al, 1992), DNA hybridization (Attathom et al, 1994), colloidal textile dye-based dipstick immunoassay (Nataraju et al, 1994), and western blot analysis. PCR is an extremely sensitive technique that amplifies target DNA sequences, and PCR amplification of conserved fragments enables the detection of low levels of viral DNA (Mallika, 2006).…”

Section: Introductionmentioning

confidence: 99%

Studies on Natural and Experimental Nucleopolyhedrovirus Infection of the Silkworm, Bombyx mori (Race: PMCSR2(SL)) by PCR

Gnanavel¹,

Velu²,

Mohan³

et al. 2022

IJZI

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The silkworm, Bombyx mori L. (Bombicidae: Lepidoptera), basically a nocturnal moth, is a native of China but has long been domesticated throughout the world. The life cycle of silkworms includes various stages, viz. eggs, larval instars, pupa within silky cocoons, and adult moths. Bombyx mori nucleopolyhedrovirus (BmNPV), one of the best characterized baculoviruses, is an important pathogen in silkworms. The viral diseases are difficult to manage due to the very short life cycle of silkworms. One of the most effective solutions is the timely detection of such infections so as to stop the spread of the viral disease. In the present study, we determined the specific nucleopolyhedrovirus ~738 base pair amplified from isolated viral DNA by the polymerase chain reaction (PCR) technique.

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Colloidal Textile Dye-Based Dipstick Immunoassay for the Detection of Nuclear Polyhedrosis Virus (BmNPV) of Silkworm, Bombyx mori L.

Cited by 9 publications

References 0 publications

Bombyx mori nucleopolyhedrovirus (BmBPV): Its impact on silkworm rearing and management strategies

Bombyx mori nucleopolyhedrovirus (BmBPV): Its impact on silkworm rearing and management strategies

Prevalence and molecular characterization of viruses causing diseases in Bombyx mori L. (Lepidoptera: Bombycidae) from different climatic regions of India

Studies on Natural and Experimental Nucleopolyhedrovirus Infection of the Silkworm, Bombyx mori (Race: PMCSR2(SL)) by PCR

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