Colocalization of aldolase and FBPase in cytoplasm and nucleus of cardiomyocytes

Mamczur, Piotr; Duś, Danuta; Dżugaj, Andrzej

doi:10.1016/j.cellbi.2007.03.029

Cited by 14 publications

(11 citation statements)

References 44 publications

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“…We have found muscle FBPase in the nuclei of cardiomyocytes , smooth muscle cells (Gizak et al, 2005) and satellite cells (Gizak et al, 2006). We postulated that in the nuclei of satelite cells muscle FBPase may be involved in cell differentiation, but the physiological role of this isozyme in the nuclei of cardiomyocytes is unknown, although its participation in glyconeogenesis cannot be excluded (Mamczur et al, 2007). No effect of calcium ions on the nuclear localization of FBPase in cardiomyocytes were observed here.…”

Section: Discussioncontrasting

confidence: 44%

Regulation of subcellular localization of muscle FBPase in cardiomyocytes. The decisive role of calcium ions.

Majkowski

Wypych²,

Pomorski³

et al. 2010

Acta Biochim Pol

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Glyconeogenesis, the synthesis of glycogen from carbohydrate precursors like lactate, seems to be an important pathway participating in replenishing glycogen in cardiomyocytes. Fructose-1,6-bisphosphatase (FBPase), an indispensible enzyme of glyconeogenesis, has been found in cardiomyocytes on the Z-line, in the nuclei and in the intercalated discs. Glyconeogenesis may proceed only when FBPase accumulates on the Z-line. Searching for the mechanism of a FBPase regulation we investigated the effects of the calcium ionophore A23187, a muscle relaxant dantrolene, glucagon, insulin and medium without glucose on the subcellular localization of this enzyme in primary culture of neonatal rat cardiomyocytes. Immunofluorescence was used for protein localization and the intracellular calcium concentration was measured with Fura. We found that the concentration of calcium ions was the decisive factor determining the localization of muscle FBPase on the Z-line. Calcium ions had no effect on the localization of the enzyme in the intercalated discs or in the nuclei, but accumulation of FBPase in the nuclei was induced by insulin.

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Section: Discussioncontrasting

confidence: 44%

Regulation of subcellular localization of muscle FBPase in cardiomyocytes. The decisive role of calcium ions.

Majkowski

Wypych²,

Pomorski³

et al. 2010

Acta Biochim Pol

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“…Chung et al [ 24 ] nicely demonstrated the intercalation of the mitochondria with the myofibrils using line scan analysis. We have now taken this one step further and quantified the level of intercalation using Pearson correlation coefficient, which is frequently used in biometrics to provide a measure of colocalization of two fluorescent signals [ 51 ] but in this case was used to indicate the amount of separation (or exclusion) of two signals. The scatter graphs shown in Figure 4 illustrate the level of intercalation along line scans from 4 distinct regions in each of 20 cells for each of the untreated controls and the various isoproterenol and oleic acid treatments.…”

Section: Discussionmentioning

confidence: 99%

Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells

Chan

Keung

Tse

et al. 2015

Stem Cells International

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Quantitative methods were established to determine the level of maturation of human embryonic stem cell-derived ventricular cardiomyocytes (hESC-vCMs) that were treated with different metabolic stimulants (i.e., isoproterenol and oleic acid) during early differentiation. Cells were double-immunolabeled with α-actinin and COX IV antibodies, to label the myofibrils and mitochondria, respectively, after which images were acquired via confocal microscopy. In order to determine the extent of differentiation, image analysis protocols were then used to quantify cell shape and area, as well as the degree of myofibrillar organization and intercalation of mitochondria between the myofibrils within the cells. We demonstrated that oleic acid or isoproterenol alone, or a combination of the two, induced a more elongated hESC-vCM phenotype than the untreated controls. In addition, cells treated with isoproterenol alone exhibited a similar level of myofibrillar organization as the controls, but those treated with oleic acid with/without isoproterenol exhibited a more organized (parallel) orientation of myofibrils. The combined isoproterenol/oleic acid treatment also resulted in enhanced intercalation of mitochondria between the myofibrils. We suggest that these quantitative morphometric methods might serve as simple and effective tools that can be utilized in the determination of the level of structural maturation of hESC-vCMs.

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“…Previously, aldolase has been found in the nuclei of several cell types in which mitosis or karyokinesis occurs: in smooth muscle cells (Mamczur and Dzugaj 2008), cardiomyocytes (Mamczur et al 2007), and hepatocytes (Sáez and Slebe 2000). Hence, we are tempted to assume that the retinal aldolase is a part of the cell division machinery.…”

Section: Discussionmentioning

confidence: 99%

Ubiquitous presence of gluconeogenic regulatory enzyme, fructose-1,6-bisphosphatase, within layers of rat retina

2010

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To shed some light on gluconeogenesis in mammalian retina, we have focused on fructose-1,6-bisphosphatase (FBPase), a regulatory enzyme of the process. The abundance of the enzyme within the layers of the rat retina suggests that, in mammals in contrast to amphibia, gluconeogenesis is not restricted to one specific cell of the retina. We propose that FBPase, in addition to its gluconeogenic role, participates in the protection of the retina against reactive oxygen species. Additionally, the nuclear localization of FBPase and of its binding partner, aldolase, in the retinal cells expressing the proliferation marker Ki-67 indicates that these two gluconeogenic enzymes are involved in non-enzymatic nuclear processes.Electronic supplementary materialThe online version of this article (doi:10.1007/s00441-010-1008-2) contains supplementary material, which is available to authorized users.

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Colocalization of aldolase and FBPase in cytoplasm and nucleus of cardiomyocytes

Cited by 14 publications

References 44 publications

Regulation of subcellular localization of muscle FBPase in cardiomyocytes. The decisive role of calcium ions.

Regulation of subcellular localization of muscle FBPase in cardiomyocytes. The decisive role of calcium ions.

Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells

Ubiquitous presence of gluconeogenic regulatory enzyme, fructose-1,6-bisphosphatase, within layers of rat retina

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