Colocalization of arginine-vasotocin and chicken luteinizing hormone-releasing hormone-I (cLHRH-I) in the preoptic–hypothalamic region of the chicken

Dhondt, Elisabeth; Eelen, Marianne; Berghman, Luc; Vandesande, Frans

doi:10.1016/s0006-8993(99)02271-4

Cited by 15 publications

(2 citation statements)

References 58 publications

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“…The concentrations of AVP‐ and TRH‐like immunoreactivity (‐LI) were measured in duplicate by means of double‐antibody RIA methods. The production and characterization of antibodies against AVP (generously provided by Dr F. Vandesande, Katholieke Universiteit, Leuven, Belgium) and TRH (#4B17 generously provided by Dr D. Grouselle, INSERM U 519, Centre Paul Broca, Paris, France) have been described previously (21, 22).…”

Section: Methodsmentioning

confidence: 99%

Effect of Prolyl Endopeptidase Inhibition on Arginine‐Vasopressin and Thyrotrophin‐Releasing Hormone Catabolism in the Rat Brain

Bellemère

Vaudry

Morain

et al. 2005

J Neuroendocrinology

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Compound S 17092 is a potent and selective inhibitor of prolyl endopeptidase (EC 3.4.21.26, PEP) that may be of therapeutic value for the treatment of memory impairment associated with neurodegenerative diseases. In the present study, we investigated the effects of S 17092 on the catabolism of the promnesic neuropeptides thyrotrophin-releasing hormone (TRH) and arginine-vasopressin (AVP) in the rat brain. In vitro, bacterial PEP hydrolysed both TRH and AVP, and the breakdown of the two peptides was almost completely prevented by 10(-5) M S 17092. In vivo, a single oral administration of S 17092 provoked a significant increase in TRH-like immunoreactivity (TRH-LI) in the cerebral cortex (+63% for a 10 mg/kg dose and +72% for a 30 mg/kg dose), as well as AVP-LI in the hippocampus (+54% for a 30 mg/kg dose), but did not affect TRH-LI in the amygdala nor AVP-LI in the cerebral cortex. Chronic administration of S 17092 (10 or 30 mg/kg daily) lead to a significant increase in THR-LI in the cerebral cortex (+55% and +56%, respectively), but did not modify AVP-LI in the hippocampus, nor in the cerebral cortex. These results show that the selective PEP inhibitor S 17092 increases TRH and AVP content in discrete regions of the rat brain. The present data suggest that the promnesic and antiamnesic effects of S 17092 can be accounted for, at least in part, by blockage of AVP and TRH degradation by PEP.

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Section: Methodsmentioning

confidence: 99%

Effect of Prolyl Endopeptidase Inhibition on Arginine‐Vasopressin and Thyrotrophin‐Releasing Hormone Catabolism in the Rat Brain

Bellemère

Vaudry

Morain

et al. 2005

J Neuroendocrinology

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“…Sections were thaw-mounted onto SuperFrost Plus slides (Fisher Scientific), dried at 37°C, and stored at −30°C until use. The anatomy of the chick brain was determined according to Puelles et al (2007) [43] and the nomenclature adopted in this study was based on this atlas and previous studies [44], [45], [46].…”

Section: Methodsmentioning

confidence: 99%

A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds

et al. 2012

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A mammalian type opsin 5 (neuropsin) is a recently identified ultraviolet (UV)-sensitive pigment of the retina and other photosensitive organs in birds. Two other opsin 5-related molecules have been found in the genomes of non-mammalian vertebrates. However, their functions have not been examined as yet. Here, we identify the molecular properties of a second avian opsin 5, cOpn5L2 (chicken opsin 5-like 2), and its localization in the post-hatch chicken. Spectrophotometric analysis and radionucleotide-binding assay have revealed that cOpn5L2 is a UV-sensitive bistable pigment that couples with the Gi subtype of guanine nucleotide-binding protein (G protein). As a bistable pigment, it also shows the direct binding ability to agonist all-trans-retinal to activate G protein. The absorption maxima of UV-light-absorbing and visible light-absorbing forms were 350 and 521 nm, respectively. Expression analysis showed relatively high expression of cOpn5L2 mRNA in the adrenal gland, which is not photoreceptive but an endocrine organ, while lower expression was found in the brain and retina. At the protein level, cOpn5L2 immunoreactive cells were present in the chromaffin cells of the adrenal gland. In the brain, cOpn5L2 immunoreactive cells were found in the paraventricular and supraoptic nuclei of the anterior hypothalamus, known for photoreceptive deep brain areas. In the retina, cOpn5L2 protein was localized to subsets of cells in the ganglion cell layer and the inner nuclear layer. These results suggest that the non-mammalian type opsin 5 (Opn5L2) functions as a second UV sensor in the photoreceptive organs, while it might function as chemosensor using its direct binding ability to agonist all-trans-retinal in non-photoreceptive organs such as the adrenal gland of birds.

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Sex and seasonal co-variation of arginine vasotocin (AVT) and gonadotropin-releasing hormone (GnRH) neurons in the brain of the halfspotted goby

Maruska

Mizobe

Tricas

2007

Comparative Biochemistry and Physiology Part A: Molecular &

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Gonadotropin-releasing hormone (GnRH) and arginine vasotocin (AVT) are critical regulators of reproductive behaviors that exhibit tremendous plasticity, but co-variation in discrete GnRH and AVT neuron populations among sex and season are only partially described in fishes. We used immunocytochemistry to examine sexual and temporal variations in neuron number and size in three GnRH and AVT cell groups in relation to reproductive activities in the halfspotted goby (Asterropteryx semipunctata). GnRH-immunoreactive (-ir) somata occur in the terminal nerve, preoptic area, and midbrain tegmentum, and AVT-ir somata within parvocellular, magnocellular, and gigantocellular regions of the preoptic area. Sex differences were found among all GnRH and AVT cell groups, but were time-period dependent. Seasonal variations also occurred in all GnRH and AVT cell groups, with coincident elevations most prominent in females during the peak- and non-spawning periods. Sex and temporal variability in neuropeptide-containing neurons are correlated with the goby's seasonally-transient reproductive physiology, social interactions, territoriality and parental care. Morphological examination of GnRH and AVT neuron subgroups within a single time period provides detailed information on their activities among sexes, whereas seasonal comparisons provide a fine temporal sequence to interpret the proximate control of reproduction and the evolution of social behavior.

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Colocalization of arginine-vasotocin and chicken luteinizing hormone-releasing hormone-I (cLHRH-I) in the preoptic–hypothalamic region of the chicken

Cited by 15 publications

References 58 publications

Effect of Prolyl Endopeptidase Inhibition on Arginine‐Vasopressin and Thyrotrophin‐Releasing Hormone Catabolism in the Rat Brain

Effect of Prolyl Endopeptidase Inhibition on Arginine‐Vasopressin and Thyrotrophin‐Releasing Hormone Catabolism in the Rat Brain

A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds

Sex and seasonal co-variation of arginine vasotocin (AVT) and gonadotropin-releasing hormone (GnRH) neurons in the brain of the halfspotted goby

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