Colocalization of dopamine and serotonin in the rat pituitary gland and in the nuclei innervating it

Vanhatalo, Sampsa; Soinila, Seppo; Kaartinen, Kimmo; Back, Nathan

doi:10.1016/0006-8993(94)01276-n

Cited by 31 publications

(11 citation statements)

References 34 publications

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“…5-HT immunoreactivity has been reported to be transiently present in the developing rat hypothalamus (Gaspar et al, 2003;Hansson et al, 1998;Ugrumov et al, 1989) and in the adult hypothalamus under experimental conditions (Beaudet and Descarries, 1979;Chan-Palay, 1977;Kent and Sladek, 1978;Vanhatalo et al, 1995). However, at least some of these neurons most likely take up 5-HT from the surroundings rather than being bona fide 5-HT synthesizing neurons since the acquisition of 5-HT immunoreactivity was prevented by co-injections of fluoxetine, a selective 5-HT reuptake inhibitor (Vanhatalo and Soinila, 1998).…”

Section: Posterior Tuberculum/hypothalamic Populationsmentioning

confidence: 95%

The serotonergic system in fish

Lillesaar

2011

Journal of Chemical Neuroanatomy

267

246

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Section: Posterior Tuberculum/hypothalamic Populationsmentioning

confidence: 95%

The serotonergic system in fish

Lillesaar

2011

Journal of Chemical Neuroanatomy

267

246

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“…The sections were rinsed in phosphate‐buffered saline (PBS, pH 7,4), incubated with 5% normal swine serum in PBS‐Triton X‐100 (0.25%) for 20 min, with primary antiserum in PBS‐Triton overnight at 4 °C, rinsed in PBS and incubated with the secondary antiserum for 1 h at 20 °C. Antibody dilutions were 1 : 500 for the polyclonal rabbit antiserotonin (Vanhatalo et al . 1994), 1 : 400–500 for the polyclonal sheep anti‐TpOH (Chemicon Inc., Temecula, CA, USA, Lot nos AB148 and AB 1541) (Cash et al .…”

Section: Methodsmentioning

confidence: 99%

“…As to the specificity of the immunohistochemistry, we have previously shown that our serotonin antibody is sensitive to serotonin–protein conjugates (recognizes 1 ng of conjugate) and it does not recognize even as high as a 1000‐fold amount of catecholamines (Soinila & Mpitsos 1991; Vanhatalo et al . 1994).…”

Section: Methodsmentioning

confidence: 99%

“…A number of studies have been conducted to reveal the details in the anatomy of the serotonergic nerves in the hypothalamus (Steinbusch & Nieuwenhuys 1981; Steinbusch 1984; Ugrumov et al . 1986; Vanhatalo et al . 1994; Vanhatalo & Soinila 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The expression of other transmitters or the identity of these neurons has not yet been revealed in adult animals, while we and others have shown that they are not catecholamine‐synthesizing neurons (Arezki et al . 1987; Vanhatalo et al . 1994).…”

Section: Introductionmentioning

confidence: 99%

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Serotonin is not synthesized, but specifically transported, in the neurons of the hypothalamic dorsomedial nucleus

Vanhatalo

Soinila

1998

Eur J of Neuroscience

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A small group of neurons in the hypothalamic dorsomedial nucleus (DMN) have been reported to contain serotonin after pharmacological treatments enhancing brain serotonin levels. This study aimed at elucidating whether these neurons are able to synthesize serotonin de novo, and whether they possess a specific serotonin transport mechanism. Serotonin content in these neurons was raised by administration of L-tryptophan and pargyline. Double immunostaining for serotonin and tryptophan hydroxylase (TpOH), the serotonin synthesizing enzyme, revealed that none of the serotonin-containing neuronal somata expressed TpOH. Intracerebroventricular colchicine treatment did not result in TpOH-IR in these neurons. Fluoxetine, a specific serotonin transport inhibitor, prevented the accumulation of serotonin in these neurons. The present results thus indicate that the serotonin-containing DMN neurons are not able to synthesize serotonin. Instead, they take up exogenous serotonin via a specific serotonin transport mechanism. As serotonin and DMN are associated with various physiological functions, such as regulation of food intake and modulation of fear and anxiety, the mechanisms revealed in the present study may participate in these clinically important brain functions.

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Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish,Danio rerio, using transgenics to visualize raphe‐specificpet1expression

Lillesaar

Stigloher

Tannhäuser

et al. 2008

J of Comparative Neurology

141

115

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Serotonin is a major central nervous modulator of physiology and behavior and plays fundamental roles during development and plasticity of the vertebrate central nervous system (CNS). Understanding the developmental control and functions of serotonergic neurons is therefore an important task. In all vertebrates, prominent serotonergic neurons are found in the superior and inferior raphe nuclei in the hindbrain innervating most CNS regions. In addition, all vertebrates except for mammals harbor other serotonergic centers, including several populations in the diencephalon. This, in combination with the intricate and wide distribution of serotonergic fibers, makes it difficult to sort out serotonergic innervation originating from the raphe from that of other serotonergic cell populations. To resolve this issue, we isolated the regulatory elements of the zebrafish raphe-specific gene pet1 and used them to drive expression of an eGFP transgene in the raphe population of serotonergic neurons. With this approach together with retrograde tracing we 1) describe in detail the development, anatomical organization, and projection pattern of zebrafish pet1-positive neurons compared with their mammalian counterparts, 2) identify a new serotonergic population in the ventrolateral zebrafish hindbrain, and 3) reveal some extent of functional subdivisions within the zebrafish superior raphe complex. Together, our results reveal for the first time the specific innervation pattern of the zebrafish raphe and, thus, provide a new model and various tools to investigate further the role of raphe serotonergic neurons in vertebrates.

show abstract

Colocalization of dopamine and serotonin in the rat pituitary gland and in the nuclei innervating it

Cited by 31 publications

References 34 publications

The serotonergic system in fish

The serotonergic system in fish

Serotonin is not synthesized, but specifically transported, in the neurons of the hypothalamic dorsomedial nucleus

Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish,Danio rerio, using transgenics to visualize raphe‐specificpet1expression

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