Colonization Factor Antigens of Human Pathogens

Dj, Evans; Dg, Evans

doi:10.1007/978-3-642-74703-8_7

Cited by 12 publications

(9 citation statements)

References 55 publications

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“…Binding by mucin retards the access of microorganisms to the surface of the epithelial cells of the mucosa and favors their removal (21). On the other hand, some motile microorganisms such as H. pylori may use their temporary attachment to mucin as a means of "tracking" toward the epithelium, where they colonize and secrete virulence factors such as cytotoxins (10).…”

Section: Discussionmentioning

confidence: 99%

Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin

Namavar¹,

Sparrius²,

Veerman

et al. 1998

Infect Immun

119

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The in vitro binding of surface-exposed material and outer membrane proteins of Helicobacter pylori to high-molecular-weight salivary mucin was studied. We identified a 16-kDa surface protein which adhered to high-molecular-weight salivary mucin. This protein binds specifically to sulfated oligosaccharide structures such as sulfo-Lewis a, sulfogalactose and sulfo-N-acetyl-glucosamine on mucin. Sequence analysis of the protein proved that it was identical to the N-terminal amino acid sequence of neutrophil-activating protein. Moreover, this adhesin was able to bind to Lewis x blood group antigen.

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Section: Discussionmentioning

confidence: 99%

Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin

Namavar¹,

Sparrius²,

Veerman

et al. 1998

Infect Immun

119

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“…Virulence by ETEC is also in part contributed by the acquisition of the plasmid for the pili or fimbriae colonization factor Ags, which enhance the colonization of E. coli in the gastrointestinal tract. The ETEC pili are a heterogenous group of fimbrial adhesins and are responsible for adherence to small intestinal epithelium via their fimbriae or long, hair-like projections extending from the bacterial cell surface to epithelial mannose-containing glycoproteins (5). Previous studies show that oral delivery of purified colonization factor Ags fails to induce significant serum IgG or secretory IgA (S-IgA) Abs (6), and these anti-fimbriae Abs (7,8) fail to protect human volunteers from pathogenic ETEC challenge (7).…”

Section: E Nterotoxigenic Escherichia Coli (Etec)mentioning

confidence: 99%

A Live Diarrheal Vaccine Imprints a Th2 Cell Bias and Acts as an Anti-Inflammatory Vaccine

Jun

Gilmore

Callis

et al. 2005

The Journal of Immunology

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An experimental vaccine for enterotoxigenic Escherichia coli (ETEC) composed of a live, attenuated Salmonella vector-expressing enterotoxigenic E. coli fimbriae, colonization factor Ag I (CFA/I), stimulated a biphasic Th cell response when given orally and suppressed the normally produced proinflammatory response. Such suppression was also evident upon the Salmonella-CFA/I infection of macrophages resulting in diminished TNF-α, IL-1, and IL-6 production and suggesting that the CFA/I fimbrial expression by Salmonella may protect against a proinflammatory disease. To test this hypothesis, SJL/J mice were vaccinated with Salmonella-CFA/I construct 1 or 4 wk before induction of experimental autoimmune encephalomyelitis using an encephalitogenic proteolipid protein peptide, PLP139–151. Mice receiving Salmonella-CFA/I vaccine recovered completely from mild acute clinical disease and showed only mild inflammatory infiltrates in the spinal cord white and gray matter. This protective effect was accompanied by a loss of encephalitogenic IFN-γ-secreting Th cells and was replaced with an increase in IL-4, IL-10, and IL-13 secretion. Collectively, these data suggested that Salmonella-CFA/I is an anti-inflammatory vaccine that down-regulates proinflammatory cells and confers protection against a proinflammatory disease, experimental autoimmune encephalomyelitis, via immune deviation.

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“…However, the conclusion drawn from the ELISA data is that feeding facilitates Flavobacterium branchiophilum colonization of the gill. Bacterial colonization is defined as the attachment of bacteria to a specific site and subsequent multiplication at that site (Evans & Evans 1990). Since the amount of antigen on the gill was similar in both the fed and the unfed fish immediately post-exposure, it is most likely that the influence of feeding was on F. branchiophilum multiplication following attachment.…”

Section: Influence Of Water Qualitymentioning

confidence: 99%

Influence of feeding on the development of bacterial gill disease in rainbow trout Oncorhynchus mykiss

MacPhee¹,

Ostland²,

Lumsden³

et al. 1995

Dis. Aquat. Org.

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Three trials were conducted to determine if feeding influenced the development of bacterial gill disease (BGD) in fingerling rainbow trout Oncorhynchus mykiss following bath exposure to Flavobacterium branchiophilum (LAB 4a). In each trial (Trial 1. n = 70 X 3; Trial 2, n = 140 X 3 ; Trial 3, n = 250 X 3), the comparison groups (fed, unfed, negative controls) were held in similar conditions and (where applicable) fed prior to exposure to the pathogen. Severe clinical signs and high rates of mortality developed within 24 h in fish which were provided feed following the bath exposure. Cumulative mortalities ranged from 43 to 63% by 12 d post-exposure Unfed fish demonstrated only mild and transient clinical signs of BGD, and cumulative mortalities of 0 to 2 % 12 d post-exposure. In one trial, resumption of feeding 6 d post-exposure resulted in 26% cumulative mortality within 7 d. Estimation of the quantity of F branchiophilum antigen by enzyme-linked immunosorbent assay (ELISA) indicated that feeding facilitated colonization of the gill. The evaluation of water quality and direct observations suggest that the influence of feeding on BGD is linked to the consumption of feed by the fish, rather than to environmental changes arising from feeding. Alterations in the unstirred layer on the gill, secondary to feed consumption and waste excretion, may aid bacterial colonization.

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Colonization Factor Antigens of Human Pathogens

Cited by 12 publications

References 55 publications

Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin

Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin

A Live Diarrheal Vaccine Imprints a Th2 Cell Bias and Acts as an Anti-Inflammatory Vaccine

Influence of feeding on the development of bacterial gill disease in rainbow trout Oncorhynchus mykiss

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