Colonization of methicillin-resistant Staphylococcus aureus among healthcare students: an integrative review

Oliveira, Erika Morganna Neves de; Carvalho, Ana Raquel Batista de; Ferreira, Adriano Menis; Moura, Luana Kelle Batista; Valle, Andréia Rodrigues Moura da Costa; Freitas, Daniela Reis Joaquim de; Moura, Maria Eliete Batista

doi:10.1590/1516-3180.2020.0564.r2.22042021

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…The transmission of commensal Staphylococcus spp., including those resistant to methicillin and other antibiotics, has been recognized from animals to humans and vice versa [35], particularly among domestic animals and their owners [32,[36][37][38][39][40][41][42][43][44]. Importantly, the transmission also occurs among staff working in veterinary hospitals and their patients, as well as among patients who are in the same hospital [5,[45][46][47][48][49][50]. The objective of this study was to isolate and identify Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Staphylococcus Species Obtained from Healthy Companion Animals and Humans

Thomson

García

Miles

et al. 2022

Veterinary Sciences

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The close contact between people and their pets has generated the exchange of skin microbiota, accompanied by bacteria that present resistance to antibiotics. Staphylococcus spp., opportunistic pathogens present in the skin and mucosa of mammals, have had their importance recognized in human and veterinary medicine. The objectives of this study were to identify Staphylococcus spp. present in isolates from the nostrils of healthy humans, dogs and cats as well as to determine their phenotype of resistance to methicillin. Strain identification was performed by MALDI-TOF mass spectrometry and antimicrobial susceptibility was determined using a disk diffusion assay for 12 antibiotics. Sixty humans (veterinary and technicians), sixty dogs and sixty cats were sampled; of them, 61.6%, 56.6% and 46.6%, respectively, carried Staphylococcus spp. in their nostrils, and only two people carried two different species of Staphylococcus in the only anatomical site sampled. A methicillin-resistant phenotype was present in 48.7% of the humans, 26.5% of the dogs and 57.1% of the cats, and sampled. These results demonstrate the presence of Staphylococcus spp. strains resistant to methicillin in personnel who work in contact with animals, as well as in dogs and cats that entered the same hospital or veterinary clinic, which alerts us to the potential transfer of these strains to or between people, dogs and/or cats.

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Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Staphylococcus Species Obtained from Healthy Companion Animals and Humans

Thomson

García

Miles

et al. 2022

Veterinary Sciences

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Drug Resistance and Epigenetic Modulatory Potential of Epigallocatechin-3-Gallate Against Staphylococcus aureus

et al. 2022

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Background: Antimicrobial resistance of human pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), is described by the World Health Organization as a health global challenge and efforts must be made for the discovery of new effective and safe compounds. This work aims to evaluate Epigallocatechin-3-gallate (EGCG) epigenetic and drug modulatory potential against S. aureus in vitro and in vivo.Methods: S. aureus strains were isolated from commensal ora of healthy volunteers. Antibiotic susceptibility and synergistic assay were assessed trough disk diffusion accordingly to EUCAST guidelines with and without coexposure to EGCG at nal concentrations of 250, 100, 50 and 25 µg/ml. Transcriptional expression of orfx, spdC and WalKR was performed trough qRT-PCR. A 90 days interventional study was performed with a daily consumption of 225 mg of EGCG.Results: Obtained data revealed a high prevalence of S. aureus colonization in health care workers and clearly demonstrate the antimicrobial and synergistic potential of EGCG as well as divergent resistant phenotypes associated with altered transcriptional expression of epigenetic and drug response modulators genes.Conclusions: Here we demonstrate the potential of EGCG for antimicrobial treatment and/or therapeutic adjuvant against antibiotic resistant microorganisms and report divergent patterns of epigenetic modulators expression associated with phenotypic resistance pro les.

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Distribution of IcaA and IcaB Genes in Biofilm-Producing Methicillin-Resistance Staphylococcus aureus

Rawat

Malik

Bisht

et al. 2022

Asian Journal of Pharmaceutical Research and Health Care

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Background: Biofilm-producing methicillin-resistance Staphylococcus aureus (MRSA) strains may reduce the penetration rate of antibiotics which leads to treatment failure; therefore, the aim of our study was to assess the presence of icaA and icaB biofilm-producing genes in clinically isolated MRSA strains. Materials and Methods: All pus samples received in the microbiology laboratory were processed as per the standard microbiological procedure for the isolation of S. aureus. Detection of MRSA is done by the cefoxitin disc diffusion method. Phenotypic detection of biofilm in all MRSA strains was accessed by tissue culture plate (TCP) method and polymerase chain reaction as genotypic method was used for detection of icaA and icaB genes. Results: A total of 222 S. aureus were isolated from pus specimens. Out of these, 127 isolates were MRSA (57%). TCP method demonstrated that 68% of MRSA were biofilm producers, on the other hand, 52 (41%) strains showed the presence of icaA gene, and 39 (31%) strains showed the presence of icaB genes in 127 MRSA strains. Conclusion: The detection of biofilm in S. aureus should be considered as essential for better management of diseases caused by biofilm-producing S. aureus strains.

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Colonization of methicillin-resistant Staphylococcus aureus among healthcare students: an integrative review

Cited by 5 publications

References 34 publications

Isolation and Identification of Staphylococcus Species Obtained from Healthy Companion Animals and Humans

Isolation and Identification of Staphylococcus Species Obtained from Healthy Companion Animals and Humans

Drug Resistance and Epigenetic Modulatory Potential of Epigallocatechin-3-Gallate Against Staphylococcus aureus

Distribution of IcaA and IcaB Genes in Biofilm-Producing Methicillin-Resistance Staphylococcus aureus

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