Colonization of the cementum surface of teeth by oral Gram-negative bacteria

Celesk, Roger A.; McCabe, R.M.; London, Jack

doi:10.1128/iai.26.1.15-18.1979

Cited by 21 publications

(13 citation statements)

References 13 publications

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“…(b) Non-igG serum added first, foiiowed by '"i-igG (bioci^ing experiment), (c) ""i-igG added first, foiiowed by '"I-igG (reversai experiment). (Celesk, McCabe & London 1979) and, in addition, by the difference in the absorptive capacities of cementum and enamel (Fine et al 1983). The experiments described in this report attempted to develop an in vitro model for the study of interactions between cementum and gingival fluid proteins in periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

In vitro sorption of IgG to cementum

Fine

Wilton

Caravana

1984

J of Periodontal Research

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The nature of the interaction between root surfaces and gingival fluid proteins has not been studied. Therefore, a series of in vitro investigations were undertaken to develop an assay to determine sorption of prominent gingival fluid proteins to the cementum. In initial studies extracted teeth were immersed in various concentrations of whole human serum and incubated with rabbit anti‐IgG, IgA, IgM, and C3 followed by anti‐peroxidaseperoxidase bridging reagents. Of the proteins analysed IgG sorbed to the highest degree in gross and microscopic evaluations. Remaining studies focused on quantifying IgG sorption, and thus root segments of known surface area were prepared and immersed in 125I‐IgG. Sorption of IgG was calculated as 21.5 ± 0.27 ng/mm2 when IgG was added at concentrations found in gingival fluid. Root fragments immersed in non‐IgG human serum reconstituted with specific amounts of 125I‐IgG, sorped only 3.4 ± 0.9 ng/mm2 of IgG, and direct measurement of IgG sorption in whole serum by the ELISA method gave comparable results. Moreover, pre‐coating of root segments with non‐IgG serum followed by incubation with 125I‐IgG resulted in 74% inhibition of IgG binding. Results suggest that serum protein sorption to root surfaces can be quantitated and may be non‐specific and concentration dependent with respect to IgG.

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Section: Discussionmentioning

confidence: 99%

In vitro sorption of IgG to cementum

Fine

Wilton

Caravana

1984

J of Periodontal Research

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“…A Capnocytophaga-hkc strain, NS UX)1, was isolated from subgingival plaque of a patient with periodontal di.sease (2). The bacteria were grown for 48 h in Schaedler's broth (BBL -Microbiological Associates) with 10 uCI…”

Section: Bacterial Culture Conditionsmentioning

confidence: 99%

“…Capnocytophaga is tine tif Ihc bacteria istilatetl frtim peritidontal ptickets and has been shtiwn lti selectively ctilonize lhc rtitil surface in vitro (2,13). Therefore, it is not surprising to find this preferential attachment of bacteria to Ihe cemental extract.…”

Section: 140"mentioning

confidence: 99%

“…Ihc maior organic protein of cetnentum (1), or a protein extract of cetnentum obtained from healthy bovine teeth. A Capttocytophaga-\\kc strain, NS ltK)l, which attaches selectively to eemental surfaces and is asstieiated with peridontal disease was utilized (2). Recently, there have been considerable advances in the general understanding of cell attachment to their ground matrix (3), including studies of bacterial adherence to specific matrices (4,5).…”

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in vitro attachment of bacteria to extracts of cementum

Somerman

Kagermeir

Bowers

et al. 1985

J Oral Pathology Medicine

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Cementum is a specialized mineralized tissue providing for the attachment of perio‐dontal fibers to the root surface of a tooth. In periodontal disease this connective tissue attachment to the cemental surface is lost. The ability of bacteria to adhere to the root surface, an intial event in the disease process, may be influenced by the organic matrix of cementum. Therefore, an in vitro assay of cell attachment was modified to study bacterial adherence to protein extracts of cementum. Petri dishes coated with the extracts were pre‐incubated in culture media and then bacteria were added. Using this assay, Capnocytophaga‐like species, a gram negative bacterium implicated in periodontal disease, attached preferentially to dishes coated with cemental extracts when compared with Type I collagen or uncoated dishes. This assay system should prove beneficial for studying the attachment of various microorganisms to protein extracts of both normal and diseased cementum. as well as providing insight into the unique attachment properties of cementum.

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“…In this investigation an extracellular polysaccharide (EP) was purified from culture supernatants of Capnocytophaga ochracea 25, a gramnegative bacterial species found in the human oral cavity and associated with certain forms of periodontal disease (3,7,15). The modulatory effect of EP on murine splenic lymphocyte responses to the mitogens concanavalin A (ConA) and lipopolysaccharide (LPS) was evaluated in vitro.…”

Section: Modulation Of Immune Responses By Bacterial Components Has Rmentioning

confidence: 99%

Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from Capnocytophaga ochracea

Bolton

Dyer

1983

Infect Immun

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An extracellular polysaccharide was purified from culture supernatants of Capnocytophaga ochracea 25, a gram-negative bacillus associated with human periodontal disease. The extracellular polysaccharide suppressed in vitro mitogenic responses of murine splenic lymphocytes to concanavalin A and lipopolysaccharide. This suppression wad dose dependent, persisted up to 120 h, and was not caused by direct toxicity of the extracellular polysaccharide.

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Colonization of the cementum surface of teeth by oral Gram-negative bacteria

Cited by 21 publications

References 13 publications

In vitro sorption of IgG to cementum

In vitro sorption of IgG to cementum

in vitro attachment of bacteria to extracts of cementum

Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from Capnocytophaga ochracea

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