Colonization of thick-walled plant tissues by anaerobic fungi

Grenet, Élisabeth; Barry, Patrick

doi:10.1016/0377-8401(88)90051-x

Cited by 24 publications

(14 citation statements)

References 11 publications

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“…(Bauchop, 1979). The recalcitrant lignocellulosic structures that are the most difficult to degrade are generally those that are the most abundantly colonized (Akin, 1987;Bauchop, 1989;Grenet and Barry, 1988). In addition, anaerobic fungi have the necessary array of enzymes to degrade all plant cell wall polymers except lignin (Williams and Orpin, 1987;H6braud and F6vre, 1988 Bernalier et al, 1992Bernalier et al, , 1993Stewart et al, 1992;Roger ef al, 1993).…”

Section: Enzyme Assay Proceduresmentioning

confidence: 99%

Effect of anaerobic fungi on glycoside hydrolase and polysaccharide depolymerase activities, in sacco straw degradation and volatile fatty acid concentrations in the rumen of gnotobiotically reared lambs

Fonty

Ag²,

Bonnemoy³

et al. 1995

Reprod. Nutr. Dev.

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Summary ― Four naturally born lambs were placed in sterile isolators 24 h after birth, before the natural establishment of the cellulolytic microorganisms. At the age of 4 weeks, a cellulolytic bacterial population of approximately 10 a cells g-1 of rumen contents was established by inoculation with a 10-6 dilution of ruminal contents taken from an adult sheep. A pure culture of Neocallimastix frontalis MCH 3 and Piromyces communis FL was inoculated into the rumen 5 months after birth and a stable population of 10 3 -10 4 zoospores g-1 developed; the cellulolytic bacteria and fungi established populations in the 4 lambs that were similar to those observed in conventional animals. The presence of fungi led to an increase in the activity of most of the glycoside and polysaccharide hydrolases of the particle-associated microbial populations. However, this effect was not acompanied by an increase in the in sacco degradation of wheat straw or an increase in the volatile fatty acid concentration in the rumen contents.

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Section: Enzyme Assay Proceduresmentioning

confidence: 99%

Effect of anaerobic fungi on glycoside hydrolase and polysaccharide depolymerase activities, in sacco straw degradation and volatile fatty acid concentrations in the rumen of gnotobiotically reared lambs

Fonty

Ag²,

Bonnemoy³

et al. 1995

Reprod. Nutr. Dev.

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show abstract

“…Control samples of both species and samples digested in the rumen (the latter were rinsed with water after digestion) were prepared for SEM using the method described by Grenet and Barry (1988) (fig 5).…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Microscopic investigation of changes in histology and digestibility in the rumen of a forage grass and a forage legume during the first growth stage

Jamot

Grenet²

1991

Reprod. Nutr. Dévelop.

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“…On removal from the rumen after 4,8,24,48 or 72 h, the samples were rinsed in running water and fixed for 3 h at ambient temperature with 1.5 % glutaraldehyde in 0.2 M cacodylate buffer at pH 7.4. They were then post-fixed with 1 % osmic acid for 3 h and dehydrated in increasingly concentrated alcohol solutions (Grenet and Barry 1988a). These samples and three controls were then prepared as previously described (Grenet and Barry 1988a) for scanning electron microscopy (SEM).…”

Section: Microscopic Examinationsmentioning

confidence: 99%

Microbial degradation of normal maize and bm3 maize in the rumen observed by scanning electron microscopy

Grenet¹,

Barry²

1991

J Sci Food Agric

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Samples of internodes and leaf blades from normal and bm3 maize (Zea mays L ) harvested at dough to glazing stage were studied separately to determine their dry matter content, wall composition (NDF, ADF and A D L ) and digestibility in sacco. For examination by light and scanning electron microscope, fragments 0.5cm long were cut halfiay along the internode beneath the female ear and on the corresponding blade. The wall and lignin contents of the bm3 maize were lower than in normal maize. The bm3 maize had a greater extent and faster rate of internode and blade disappearance in the rumen than normal maize samples. The histological structure of the two maizes was the same, but after 24 h in the rumen the parenchyma of the bm3 maize had degraded faster and the secondary walls of the jbres of its vascular bundles were degraded whereas those of normal maize had remained intact. After 72 h in the rumen the sclerenchyma of normal maize had changed little whereas that of the bm3 maize had much thinner walls and was abundantly colonised by rumen bacteria.

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Colonization of thick-walled plant tissues by anaerobic fungi

Cited by 24 publications

References 11 publications

Effect of anaerobic fungi on glycoside hydrolase and polysaccharide depolymerase activities, in sacco straw degradation and volatile fatty acid concentrations in the rumen of gnotobiotically reared lambs

Effect of anaerobic fungi on glycoside hydrolase and polysaccharide depolymerase activities, in sacco straw degradation and volatile fatty acid concentrations in the rumen of gnotobiotically reared lambs

Microscopic investigation of changes in histology and digestibility in the rumen of a forage grass and a forage legume during the first growth stage

Microbial degradation of normal maize and bm3 maize in the rumen observed by scanning electron microscopy

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