Color cathodoluminescence display in the scanning electron microscope of deep relief surfaces

Obyden, S. K.; Ivannikov, P. V.; Saparin, G. V.

doi:10.1002/sca.4950190802

Cited by 10 publications

(5 citation statements)

References 7 publications

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“…Applications of these tools are discussed in papers by Obyden et al (1997), , and . The reference data about the chemical agents employed are given in Loginov et al (1998).…”

Section: Methodsmentioning

confidence: 99%

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The morphology and composition of cholesterol, protein, and bilirubin deposits in dried human bile: Cathodoluminescence and backscattered electron imaging

et al. 1998

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Summary: This work is the first to deal with the application of color cathodoluminescence scanning electron microscopy (CCL SEM) and a novel version of combined imaging with backscattered electrons (CCL+BSE SEM) for the study of the composition of bile and its precipitation mechanisms. The present study demonstrates cholesterol, protein, and bilirubin distribution in deposits of normal and abnormal human bile after solution evaporation to full dryness. Qualitative CCL SEM analysis showed that dried bile remnants include different proportions of the above components. Three types of deposits were observed: Arborescent crystals, typical cholesterol crystals, and amorphous bilirubin particles. The selection of crystalline or amorphous precipitate phases is determined by the dehydration/concentration process. The findings may explain key features in lithogenesis.

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“…Applications of these tools are discussed in papers by Obyden et al (1997), , and . The reference data about the chemical agents employed are given in Loginov et al (1998).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we present CCL SEM data and those from a joint cathodoluminescence and backscattered electron detector (CCL+BSE SEM). Applications of these tools are discussed in papers by Obyden et al (1997), , and . The reference data about the chemical agents employed are given in Loginov et al (1998).…”

Section: Methodsmentioning

confidence: 99%

The morphology and composition of cholesterol, protein, and bilirubin deposits in dried human bile: Cathodoluminescence and backscattered electron imaging

et al. 1998

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“…Combination of different SEMmodes data into one color image of composite contrast (Obyden et al 1997) allows one to reveal the spatial correlation between the specimen topography, location of electrical potential barriers and CL-centers distribution.…”

Section: Application Of Composite Contrast Sem-mode To the Study Of Dmentioning

confidence: 99%

Proceedings of SCANNING 2000 May 9–12, 2000 San Antonio, Texas, USA

2000

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The layer sequence of multilayered white and off-white paint chips is sometimes difficult to discern when utilizing commonly employed microscopical methods. This layer sequence information becomes vitally important when comparing a questioned paint sample to a paint sample of known origin. Techniques such as reflected light microscopy, fluorescence microscopy, and scanning electron microscopy (SEM) coupled with energy dispersive x-ray spectrometry (EDS) may not provide the needed discrimination. The elemental information obtained by EDS is of limited value since elemental composition alone is not sufficient to identify pigments/extenders used in white and offwhite paints or to distinguish between different phases. Also, binder information may not be available for comparison since the thinness of the layers and the abundance of extender pigments may preclude analysis by Fourier transform infrared (FTIR) microspectrophotometry.Cathodoluminescence microscopy (CLM) may provide the needed layer sequence information and discrimination when analyzing and comparing multilayered white and off-white paint samples. Cathodoluminescence is the emission of radiation from the sample in the visible light region and neighboring wavelengths following excitation by electrons generated from a cathode electron gun. CLM provides further discrimination of the layer sequence of multilayered white and off-white paint samples since cathodoluminescence is sensitive to phase differences, trace amounts of foreign atoms, and other lattice imperfections.Analysis of polished cross-sections by CLM was accomplished utilizing a light microscope coupled with a vacuum specimen chamber and a cold-cathode electron gun. A comparison was made of the layer information obtained by CLM, darkfield reflected light microscopy, fluorescence microscopy, and SEM-EDS of several architectural paint cross-sections.

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“…"Adobe Photoshop" software was used to separate colors and to have intensity histograms. The method and its possible fields of application were characterized in detail in our earlier publications [10][11][12]. Ten gallstones obtained from 4 SLE patients on 10-to 20-year prednisolone treatment at cholecystectomy were selected for CCL-SEM evaluation of their composition, microarchitectonics, and microstructure.…”

Section: Composition and Structure Of Gallstonesmentioning

confidence: 99%

Corticosteroids and Cholelithiasis in Systemic Lupus Erythematosus

Reshetnyak

Saparin

Ivannikov

et al. 2009

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The aim. To evaluate the frequency of gallstone formation and alteration of plasma lipid profiles in SLE patients long treated with prednisolone. Material and methods. Sixty patients with SLE were divided into 2 groups: (1) 38 SLE patients without gallstones; (2) 22 SLE patients with gallstones. Gallbladder ultrasonography was performed in all the patients, and the serum lipid profile was determined. To identify the composition and structure of gallstones obtained during cholecystectomy, color cathodoluminescence scanning electromicroscopy was used. Results. Gallstone disease was detected in 22 (36.7%) patients of the 60 examinees and in 22 (68.8%) of the 32 SLE patients receiving prednisolone therapy; whereas none of the 28 prednisolone-untreated patients was found to have the disease (P = .001). There were the most significant differences between the SLE patients with and without gallstones in the duration of administration of prednisolone and in its mean daily and mean monthly doses, and cumulative ones. Conclusion. Age at the onset of the disease, the mean daily dose of corticosteroids, and the duration of therapy with these agents are the most likely factors predisposing to gallstone disease in SLE patients. The CCL-SEM study identified predominantly the protein-cholesterol structure of gallstones.

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Color cathodoluminescence display in the scanning electron microscope of deep relief surfaces

Cited by 10 publications

References 7 publications

The morphology and composition of cholesterol, protein, and bilirubin deposits in dried human bile: Cathodoluminescence and backscattered electron imaging

The morphology and composition of cholesterol, protein, and bilirubin deposits in dried human bile: Cathodoluminescence and backscattered electron imaging

Proceedings of SCANNING 2000 May 9–12, 2000 San Antonio, Texas, USA

Corticosteroids and Cholelithiasis in Systemic Lupus Erythematosus

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