Colorimetric analysis of some diuretic drugs: hydrochlorothiazide and spironolactone

Abstract

Two convenient spectrophotometric methods were developed for the determination of hydrochlorothiazide and spironolactone. A specific colour reaction for the determination of hydrochlorothiazide is reported. One method is based on the reaction of hydrochlorothiazide with n-butylamine and cobaltous chloride in anhydrous methanol. A blue-violet colour is produced and is measured at 570 nm. A highly selective colorimetric method was adopted for the analysis of spironolactone by reacting with isoniazid forming a coloured hydrazone derivative and subsequent measurement of the coloured product at 405 nm.

Validated Chromatographic Methods for Determination of Hydrochlorothiazide and Spironolactone in Pharmaceutical Formulation in Presence of Impurities and Degradants

“…Derivative and indirect spectrophotometric methods were also used for their determination (22,23). Colorimetric determination was described (24).…”

Spectrophotometric Determination of Furosemide Drug in Different Formulations using Schiff ’s Bases

“…A single spectrophotometric method [18] for the determination of the drug involving Schiff's bases formation with single aromatic aldehyde viz. p-dimethyl amino cinnamaldehyde in 65% H 2 SO 4 containing FeCl 3 to produce an intensely color measurable at 530nm has been published.…”

“…In spite of the availability of variety of methods for the determination of furosemide which are of higher accuracy and reproducibility, however this work has been devoted to determine the Fu drug in different pharmaceuticals formulation, using a bench apparatus compared to the previously reported methods [18,31]. No need for sophisticated instruments, only a simple spectrophotometer can perform the determination effectively, using different commercially available reagents, based on the formation of different Schiff's bases produced from the reaction of Furosemide with a number of aromatic aldehydes.…”

“…Several methods have been reported for the determination of the components of this important drug (furosemide). Titrimetric methods [3][4][5][6][7], Potentiometric methods [8,9] Ultraviolet methods [10][11][12][13][14][15][16], Colorimetric methods [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35].…”

Simultaneous separation and rapid determination of spironolactone and its metabolite canrenone in different pharmaceutical formulations and urinary matrices by capillary zone electrophoresis

“…Since their development, SL and its metabolite CN have attracted lots of research and have been widely applied to clinical practice [10]. Many analytical methods have been developed for separation and determination of SL and CN, including spectrophotometry [11], colorimetry [12], and polarography [13] in the early nineties. In recent years, the most widely used were HPLC and LC-MS under APCI or ESI source conditions [14][15][16].…”