Colorimetric and Fluorometric Detection of Nucleic Acids Using Cationic Polythiophene Derivatives

Abstract

Different electrostatic interactions and conformational structures between single‐ or double‐stranded negatively charged nucleic acids bound to a surface (see picture) and water‐soluble cationic poly(3‐alkoxy‐4‐methylthiophene) derivatives (in yellow) provide the basis for a sensitive optical detection method (colorimetric or fluorometric) for oligonucleotide hybridization.

De novo generation of a transmissible spongiform encephalopathy by mouse transgenesis

“…However, by the third (n ϭ 3/4) and fourth (n ϭ 5/5) passage derived from mouse ''a'' (Fig. 2 A), RL 3 tga20 brain homogenates displayed abundant PK-resistant PrP [27][28][29][30] (Fig. 3A).…”

“…This property can differentiate strains even when propagated in congenic hosts. The pathological deposits of tg1020 mice, RL 3 tga20 mice (all four passages), and RL 3 WT mice were consistently negative for thioflavin T and Congo red, yet were stained by the amyloid-binding LCPs polythiophene acetic acid (PTAA; anionic) and polythiophene methyl imidazole (PTMI; cationic) (30,31) (Fig. 4A).…”

Conjugated Polyelectrolyte (CPE) Poly{3-[6-(N-methylimidazolium)hexyl]-2,5-thiophene} Complexed with DNA: Relation between Colloidal Level Solution Structure and Chromic Effects

“…They are also consistent with Leclerc's idea about DNA being wrapped over P1. [38][39][40] The SAXS curves of the P3ImiHT(DNA) x dilution series normalized to the overall concentration are shown in the Supporting Information and the curves of dilution series are qualitatively similar for each and for P3ImiHT(DNA) 0.5 and P3ImiHT(DNA) 1.3 . The distribution of P3ImiHT and P3ImiHT(DNA) 0.5 are similar, pointing to a 25 Å diameter, while there is a tendency towards thinner 15 Å diameter structures for P3ImiHT(DNA) 1.3 .…”

“…37 It is also possible to prepare imidazolium based block copolymers that provide a platform for microphase separated structures. 30 In a particular example, Leclerc and coworkers [38][39][40] employed the imidazolium substituted CPE, P1)), for probing anionic DNA and proteins. They introduced a sensing method based on the molecular recognition driven backbone planarization.…”

Functionalized Ni@SiO2Core/Shell Magnetic Nanoparticles as a Chemosensor for Fluoride Ion

“…[8][9][10]18,19 Heterogeneous anion sensors, based upon the immobilization of molecular anion receptors to solid supports, are technologically attractive due to their potential easiness of use and reusability.…”