Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for <i>Vibrio parahemolyticus</i> Determination

Sun, Yuhan; Duan, Nuo; Ma, Peisheng; Yao, Liang; Zhu, Xiaoyin; Wang, Zhouping

doi:10.1021/acs.jafc.8b06893

Cited by 90 publications

(40 citation statements)

References 38 publications

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“…All these demonstrated that CLICK-T has higher catalytic effects towards Cu(II)AAC than CLICK-17. The reasons maybe due to that CLICK-T has shorter length and could be easier to contact and combine with the analyte [25].…”

Section: The Feasibility Of Copper Detection By the Click-t Modified ...mentioning

confidence: 99%

A Novel Truncated DNAzyme Modified Paper Analytical Device for Point-of-Care Test of Copper Ions in Natural Waters

Wang

Hong

et al. 2022

Chemosensors

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On-site determination of trace copper ions in natural waters is of great significance to environmental monitoring, and how to develop accurate and specific point-of-care test methods is one critical issue. In the study, a paper-based analytical device (PAD) being modified with a new truncated DNAzyme (CLICK-T, which was derived from a reported DNAzyme-CLICK-17) was developed for Cu ions detection. The detection mechanism was based on Cu(II)-catalyzed azide-alkyne cycloaddition (Cu(II)AAC) reaction. It can directly conduct on-site analysis of Cu(II) ions based on fluorescent signals detected using a mobile phone. In the assay, the CLICK-T was firstly modified on the PADs. Then, water samples containing Cu ions mixed with 3-azido-7-hydroxycoumarin and 3-butyn-1-ol were instantly dripped on PADs and incubated for 20 min. Finally, the PADs were excited at 365 nm and emitted fluorescence which could be analyzed on site using smart phones. The Cu(II) concentration could be quantified through RGB analysis with the aid of iPhone APP software. The limit of detection is 0.1 µM by the naked eye due to the fact that CLICK-T exhibited a good catalytic effect on Cu(II)AAC. The Cu(II) concentration could also be directly detected without using reductant, such as ascorbic acid, which is prone to be oxidized in air. This simplifies the PDA detection process improves its efficiency. The PAD is convenient for the on-site analysis of Cu ions in natural waters.

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Section: The Feasibility Of Copper Detection By the Click-t Modified ...mentioning

confidence: 99%

A Novel Truncated DNAzyme Modified Paper Analytical Device for Point-of-Care Test of Copper Ions in Natural Waters

Wang

Hong

et al. 2022

Chemosensors

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“…Under optimal reaction conditions, a considerable linear detection range of 10 2 to 10 7 CFU/mL was observed, in addition to a low detection limit of 10 CFU/mL. Therefore, the synthesized biosensor showed good sensitivity and accuracy, making it a viable alternative for monitoring food quality without the need for more complicated operations [382].…”

Section: Food Applicationsmentioning

confidence: 94%

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.

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“…Sun et al [ 119 ] have reported a colorimetric aptasensor for detecting V. parahaemolyticus comprised of three parts: magnetic NPs (MNPs) modified with streptavidin, a biotinylated V. parahemolyticus aptamer, and a label-free ssDNA that is complementary (cDNA) to the aptamer and has a G-quadruples component. The aptamer was conjugated to MNPs via the biotin−streptavidin interaction, and then the cDNA was partly hybridized with aptamer thus allowing ssDNA to attach to the surface of the MNPs.…”

Section: Food and Water Analysismentioning

confidence: 99%

Recent advances in aptamer applications for analytical biochemistry

Zon

2022

Analytical Biochemistry

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Aptamers are typically defined as relatively short (20–60 nucleotides) single-stranded DNA or RNA molecules that bind with high affinity and specificity to various types of targets. Aptamers are frequently referred to as “synthetic antibodies” but are easier to obtain, less expensive to produce, and in several ways more versatile than antibodies. The beginnings of aptamers date back to 1990, and since then there has been a continual increase in aptamer publications. The intent of the present account was to focus on recent original research publications, i.e., those appearing in 2019 through April 2020, when this account was written. A Google Scholar search of this recent literature was performed for relevance-ranking of articles. New methods for selection of aptamers were not included. Nine categories of applications were organized and representative examples of each are given. Finally, an outlook is offered focusing on “faster, better, cheaper” application performance factors as key drivers for future innovations in aptamer applications.

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Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus Determination

Cited by 90 publications

References 38 publications

A Novel Truncated DNAzyme Modified Paper Analytical Device for Point-of-Care Test of Copper Ions in Natural Waters

A Novel Truncated DNAzyme Modified Paper Analytical Device for Point-of-Care Test of Copper Ions in Natural Waters

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Recent advances in aptamer applications for analytical biochemistry

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