Colorimetric biosensing of organophosphate pesticides using enzymatic nanoreactor built on zeolitic imdiazolate-8

Kukkar, Preeti; Kukkar, Deepak; Younis, Sherif A.; Singh, G.; Singh, Pritpal; Basu, Soumen; Kim, Ki‐Hyun

doi:10.1016/j.microc.2021.106242

Cited by 17 publications

(10 citation statements)

References 49 publications

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“…Nonmonotonic toxicity responses generally occur at low concentrations close to the tolerance threshold . The aggregation (size increase) of highly concentrated particles also contributes to nonmonotonic responses. , The decrease in ROS levels was not consistent with the expected toxicity. , As nanoenzymes, ZIFs exhibit antibacterial, anti-inflammatory, and antioxidative damage activities due to the quenching of ROS contents in cells or in vivo . − The quenching of • OH is presented in Figure S5. Compared with the control, ZIF-8 and ZIF-67 greatly quenched • OH.…”

Section: Resultsmentioning

confidence: 99%

Persistence and Recovery of ZIF-8 and ZIF-67 Phytotoxicity

Zhang

2021

Environ. Sci. Technol.

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Zeolitic imidazolate frameworks (ZIFs) have been developed quickly and have attracted considerable attention for use in the detection and removal of various pollutants. Understanding the environmental risks of ZIFs is a prerequisite to their safe application by industry and new chemical registration by governments; however, the persistence and recovery of toxicity induced by ZIFs remain largely unclear. This study finds that typical ZIFs (e.g., ZIF-8 and ZIF-67) at a concentration of 0.01−1 mg/L induce significant algal growth inhibition, plasmolysis, membrane permeability, chloroplast damage, and chlorophyll biosynthesis, and the above alterations are recoverable. Unexpectedly, a persistent decrease in reactive oxygen species (ROS) is observed due to the quenching of hydroxyl free radicals. The adverse effects of ZIF-8 are weak and easily alleviated compared with those of ZIF-67. ZIF-8 is internalized mainly by caveolae-mediated endocytosis, while ZIF-67 is internalized mainly by clathrin-mediated endocytosis. Omics studies reveal that the downregulation of mRNA associated with oxidative phosphorylation and the inhibition of chlorophyll and adenosine triphosphate (ATP) synthesis in mitochondria are related to the persistence of phytotoxicity. These findings highlight the phenomena and mechanisms of the persistence and recovery of phytotoxicity, indicating the need to reconsider the environmental risk assessments of ZIFs.

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Section: Resultsmentioning

confidence: 99%

Persistence and Recovery of ZIF-8 and ZIF-67 Phytotoxicity

Zhang

2021

Environ. Sci. Technol.

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“…rOPH was expressed and purified according to methods described by Luo et al (refer to Supporting Information). rOPH/ZIF-8 was prepared as previously reported with a slight modification . Briefly, 140 mg of 2-methylimidazole and 10 mg of rOPH were solubilized in 18 mL of deionized water (DI water) (solution A).…”

Section: Methodsmentioning

confidence: 99%

“…Enzyme immobilization is an effective method to improve the stability of bioscavengers. Specifically, multiple strategies, such as adsorption, covalent bonding, encapsulation, and copolymerization, can be used to physically attach enzymes to a solid support (carrier) to isolate enzymes from aggressive environments. − Mesoporous metal–organic frameworks (MOFs) have emerged as advanced materials for enzyme immobilization due to their tunable size, high surface area, and possible biofunctionalization. − These special properties endow MOFs with high enzyme loading, stable catalytic activity, and excellent diffusion of both substrate and product . Zeolitic imidazole framework-8 (ZIF-8), formed by coordination between 2-methylimidazole and Zn 2+ ions, is a typical and excellent MOF material for one-pot enzyme encapsulation with mild synthesis conditions and good biocompatibility and stability. , However, clearance by the immune system is still one of the major issues of enzyme/MOF nanoparticles, which may lead to attenuation of enzyme activity, necessitating further functionalization of these nanoparticles. , …”

Section: Introductionmentioning

confidence: 99%

“…22 Zeolitic imidazole framework-8 (ZIF-8), formed by coordination between 2-methylimidazole and Zn 2+ ions, is a typical and excellent MOF material for one-pot enzyme encapsulation with mild synthesis conditions and good biocompatibility and stability. 18,23 However, clearance by the immune system is still one of the major issues of enzyme/ MOF nanoparticles, which may lead to attenuation of enzyme activity, necessitating further functionalization of these nanoparticles. 24,25 The use of biological membranes to camouflage nanoparticles is a powerful "top-down" functionalization strategy.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dual-Modal Nanoscavenger for Detoxification of Organophosphorus Compounds

Zou¹,

Wang²,

Wang³

et al. 2022

ACS Appl. Mater. Interfaces

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Organophosphorus compounds (OPs) pose great military and civilian hazards. However, therapeutic and prophylactic antidotes against OP poisoning remain challenging. In this study, we first developed a novel nanoscavenger (rOPH/ZIF-8@E-Lipo) against methyl paraoxon (MP) poisoning using enzyme immobilization and erythrocyte–liposome hybrid membrane camouflage techniques. Then, we evaluated the physicochemical characterization, stability, and biocompatibility of the nanoscavengers. Afterward, we examined acetylcholinesterase (AChE) activity, cell viability, and intracellular reactive oxygen species (ROS) to indicate the protective effects of the nanoscavengers in vitro. Following the pharmacokinetic and biodistribution studies, we further evaluated the therapeutic and prophylactic detoxification efficacy of the nanoscavengers against MP in various poisoning settings. Finally, we explored the penetration capacity of the nanoscavengers across the blood–brain barrier (BBB). The present study validated the successful construction of a novel nanoscavenger with excellent stability and biocompatibility. In vitro, the resulting nanoscavenger exhibited a significant protection against MP-induced AChE inactivation, oxidative stress, and cytotoxicity. In vivo, apart from the positive therapeutic effects, the nanoscavengers also exerted significant prophylactic detoxification efficacy against single lethal MP exposure, repeated lethal MP challenges, and sublethal MP poisoning. These excellent detoxification effects of the nanoscavengers against OPs may originate from a dual-mode mechanism of inner recombinant organophosphorus hydrolase (rOPH) and outer erythrocyte membrane-anchored AChE. Finally, in vitro and in vivo studies jointly demonstrated that monosialoganglioside (GM1)-modified rOPH/ZIF-8@E-Lipo could penetrate the BBB with high efficiency. In conclusion, a stable and safe dual-modal nanoscavenger was developed with BBB penetration capability, providing a promising strategy for the treatment and prevention of OP poisoning.

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“…3 Parathion-methyl is also classi-ed as a toxicity category I (extremely toxic) insecticide by the WHO and U.S. EPA with a median lethal dose (LD 50 ) value of <5 mg kg À1 . 4,5 The excessive and inappropriate use of parathionmethyl causes the accumulation of pesticides in food products. Therefore, it is challenging and urgent to control parathionmethyl residues.…”

Section: Introductionmentioning

confidence: 99%