Colorimetric detection of apoptosis based on caspase-3 activity assay using unmodified gold nanoparticles

Abstract

A new label-free method for the detection of apoptosis was proposed based on colorimetric assay of caspase-3 activity using an unlabeled Asp-Glu-Val-Asp (DEVD)-containing peptide substrate and unmodified gold nanoparticles (AuNPs).

MicroRNA let-7c inhibits Bcl-xl expression and regulates ox-LDL-induced endothelial apoptosis

“…The Caspases, especially Caspase-3, are known to act downstream of Bcl-xl:Bax and play a key role in the execution of apoptosis (12). The activity of Caspase-3 would give an index to the process of apoptosis (13). MicroRNAs (miRNAs) are a family of highly conserved, small noncoding RNAs that posttranscriptionally repress gene expression via degradation or translational inhibition of their target mRNAs.…”

Sensitive cell apoptosis assay based on caspase-3 activity detection with graphene oxide-assisted electrochemical signal amplification

“…Gold nanoparticles and peptide (Pan et al, 2012) 10000 Electrochemistry DEVD-pNA (Takano et al, 2014) 1090.9 (0.01 U/mL) This study 0.06…”

“…Therefore, caspase-3 is the most used target to detect apoptosis (Du et al, 2004;Porter and Janicke, 1999). Based on the fact that the tetra-peptide substrate DEVD (Asp-Glu-Val-Asp) can be specifically recognized and cleaved at the N-terminal of the motif by caspase-3, several fluorometric and colorimetric assaying methods have been established (Boeneman et al, 2009;Jun et al, 2009;Pan et al, 2012;H. Wang et al, 2011;Zhou et al, 2014), and commercially kits are now available for caspase-3 based apoptosis assays.…”

Nanoparticle-based Caspase Sensors

“…Caspase-3 GNP Colorimetry [12] Park et al (2012) Caspase-3, -8 and -9 GNP Fluorescence (restored) [13] Sun et al (2010) Caspase-3 GNP Fluorescence (restored) [14] Oishi et al amplification. Under optimal conditions, the detection limit of caspase-3 was 0.89 nM.…”