Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay

Advances in nanomaterials and their applications in point of care (POC) devices for the diagnosis of infectious diseases

“…• achieved a lower LOD (for tap water samples) than other reported methods such as AuNP-based immunosensors (Hejazi et al, 2008) or fluorescence-based assay using CdSe quantum dots (Yang and Lai, 2011) Optical (Chen et al, 2014) Analyte: detection of HEV RNA Sample pretreatment:…”

“…Among them, gold and silver NPs are the better options, since they are less susceptible to oxidation than their copper counterparts (Jain et al, 2008). Reportedly, nanorods (Bi et al, 2015) and AuNPs were employed in a colorimetric assay for the diagnosis of Hepatitis E virus (HEV) (Chen et al, 2014). To ensure that the concentration of the biomarker fell within detectable range, real-time loopmediated isothermal amplification (RT-LAMP) was employed as part of sample preparation process.…”

“…(a) Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay(Chen et al, 2014); (b)(Tang et al, 2010) (Figure 2was under DNA section, but this system detects whole pathogens); (c) Nano metal-organic framework (NMOF)-based strategies for multiplexed microRNA detection in solution and living cancer cells(Lou et al, 2011).…”

Rapid and simple detection of Japanese encephalitis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick

“…This inhibits their application in some rural or remote areas. The LAMP method, which was first described in 2000, is an effective method for detecting many different pathogens, including mink enteritis virus (MEV; Wang et al, 2013), hepatitis E virus (HEV; Chen et al, 2014), and Listeria monocytogenes . Detection of JEV using the RT-LAMP method has been reported by Toriniwa and Komiya (2006), and it was improved by Chen et al (2011) by adding SYBR Green I after reaction to allow the results to be observed by naked eye.…”

Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

“…Since a first microfluidic disc for detection of hepatitis B virus reached an analytical sensitivity of about 50 copies per reaction within 60 min measured by turbidity [38], a significant number of POC devices based on LAMP have been developed: that is, a multichannel microfluidic device based on LAMP turbidity with a need of only 0.4 ml for detection of the swine virus in less than 1 h [39] or a multiplex microfluidic RT-LAMP device able to distinguish three types of influenza A substrains with a detection limit of 20 copies per reaction in 30 min [40]. In a similar way, a colorimetric device based on a RT-LAMP assay has been reported to detect hepatitis E virus by visual detection with a limit of detection of 10 copies and low handling and equipment requirement [41]. Last year a completely automated POC device termed Microfluidic Biomolecular Amplification Reader was reported to process raw samples through combination of molecular, microfluidic, optical and electronic technologies.…”