Colorimetric Detection of Some Highly Hydrophobic Flavonoids Using Polydiacetylene Liposomes Containing Pentacosa-10,12-diynoyl Succinoglycan Monomers

Lee

et al. 2018

Macro Materials & Eng

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The rapid detection of foot‐and‐mouth disease virus (FMDV) is vital for the prevention of foot‐and‐mouth disease outbreaks. In this study, a polyvinylidene difluoride (PVDF)‐supported polydiacetylene (PDA) immunosensor is developed to detect FMDV, in which a polyclonal antibody against the FMDV VP1 antigen is conjugated as a specific virus‐binding module without a linker. First, a liposome‐based immunosensor is generated for the FMDV VP1 protein in the form of photopolymerized PDA colloids. Then, the VP1‐specific PDA immunosensors are modified onto PVDF strip to enable the rapid and portable detection of FMDV. Detailed analyses are performed using ultraviolet‐visible spectroscopy, dynamic light scattering, transmission electron microscopy, and scanning electron microscopy. A blue‐to‐red color transition is observed in the presence of FMDV particles, indicating the potential applications of FMDV‐specific PDA immunosensors for use in solid‐phase detection as well as via liquid‐phase liposome platforms. Thus, this work provides a rapid and simple detection for FMDV.

“…The synthetic procedure of NHS‐PCDA was described in the following reference . First, 1 g of PCDA (2.7 mmol) was dissolved in 10 mL of dichloromethane.…”

Section: Methodsmentioning

confidence: 99%

Detection of Foot‐and‐Mouth Disease Virus Using a Polydiacetylene Immunosensor on Solid‐Liquid Phase

Jeong

Lee

et al. 2018

Macro Materials & Eng

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“…8F ). Although the guest is captured by the β-CD cavity, the simple binding event cannot affect the pi orbital twist on the PDA array and thereby cannot generate any response of the vesicle 38 . Accordingly, we have designed the channel on the PDA array, and the incorporated cationic amino acids induce the triggering of the ene-yne backbone via a strong electrostatic interactions and hydrogen bonds.…”

Section: Resultsmentioning

confidence: 99%

Polydiacetylenyl β-cyclodextrin based smart vesicles for colorimetric assay of arginine and lysine

Kim

Choi

et al. 2016

Sci Rep

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Selective visualization of arginine and lysine has been explored among 20 amino acids using the hybrid conjugate of β-cyclodextrin (β-CD) and polydiacetylene (PDA). The mono pentacosa-10,12-diynyl aminomethyl group was successfully coupled to either the primary or the secondary face of β-CD, where mono-6-amino-6-deoxy-β-CD or mono-3-amino-3-deoxy-β-CD reacted with the N-hydroxysuccinimide ester of 10,12-pentacosadiynoic acid. In this combinatorial system, the cylindrical β-cyclodextrin functions as a channel for the introduction of the cationic amino acids to the artificial membrane. The membrane perturbation and aggregation by the target amino acids could be exclusively visualized as a blue to red color change based on the responsive polydiacetylene domain. These interesting findings demonstrated that the developed β-CD conjugated PDA system may offer a new method of cell-penetrating mechanism, a promising vector system, as well as impact the production industry of arginine or lysine.

“…NH 2 ‐PCDA (26 mg, 60 μmoL) was dissolved in 700 μL of dimethyl sulfoxide at 65 °C for 10 min . Acetic acid at a volume of 300 μL and sodium cyanoborohydride (31.5 mg, 500 μmoL) were added to the solution.…”

Section: Methodsmentioning

confidence: 99%

Succinoglycan Octasaccharide Conjugated Polydiacetylene‐Doped Alginate Beads for Barium (II) Detection

Yun

Dindulkar

et al. 2016

Macro Materials & Eng

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chain, the external stimuli (temperature, pH, solvent, and ligand receptor interactions) causes the accumulation of structural disorder in the conjugated ene-yne array. [1] By the conformational changes, PDA undergoes blue to red color transition and the red phase PDA also emits red fluorescence. [2,3] Consequently, various PDA vesicles have been developed as attractive sensor matrices for the detection of influenza virus, [4] cholera toxin, [5] and receptor proteins. [6] However, the vesicle sensor system has a few limitations such as dilution problems when target compounds with low solubility are applied to the solution system, long term storage stability, and sensitivity problems due to vesicular aggregation. [7] Therefore, the immobilization of PDA-based sensor systems using microfibers, [8] microbeads, [9,10] and film [11] has been studied. In particular, alginate beads can be prepared in mild gelation conditions forming egg box junction with calcium ion based on the alginate structure, and have been widely used as a nontoxic and biocompatible matrix platform. [12] We then Detection methods for heavy metals are important and highly required due to their toxicity to the health of humans and the environment. Hence, using a polydiacetylene (PDA)-based sensoring bead, the presence of barium ions is exclusively detected for the first time. The sensoring platform has been designed as a PDA vesicle functionalized with a succinoglycan octasaccharide subunit serving as a metal coordination ligand. First, the succinoglycan octasaccahride subunit is isolated from Sinorhizobium meliloti, successfully conjugated to pentacosa-10,12-diynoic acid via reductive amination, and the functionalized vesicle system is investigated for color and fluorescence changes targeting nine different metal ions. To further improve the long-term storage stability and convenient handling of the vesicle detection system, the sensory vesicle is immobilized on millimeter-sized alginate beads through the ionotropic gelation method. This study provides an opportunity to design and develop various carbohydrate-based sensor materials.